1,5-Dicaffeoylquinic acid-mediated glutathione synthesis through activation of Nrf2 protects against OGD/reperfusion-induced oxidative stress in astrocytes
Abstract
Oxidative stress plays an important role in pathological processes of cerebral ischemia followed by reperfusion. The effect of 1, 5-dicaffeoylquinic acid (1, 5-diCQA) on primary culture rat cortical astrocytes induced by oxygen and glucose deprivation (OGD)/reperfusion was evaluated in this study. Appropriate concentration of 1, 5-diCQA pretreatment significantly suppressed cell death, reduced the production of reactive oxygen species, prevented glutathione (GSH) depletion, increased the activity of glutamate-cysteine ligase (GCL), and triggered Nrf2 nuclear translocation in astrocytes induced by 4h of OGD and 20 h of reperfusion. Interestingly, these protective effects were greatly attenuated in Nrf2 siRNA-transfected cells. We conclude that 1, 5-diCQA has antioxidant signaling properties that upregulate GSH synthesis by stimulating the Nrf2 pathway in astrocytes and protects them from cell death in an in vitro model of ischemia/reperfusion.
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Caffeoylquinic acids found in CA induce Nrf2 and Ho-1 signaling and positively impact glutathione levels.
“Caffeoylquinic acids found in CA [28,96,97], are also known to induce Nrf2 and Ho-1 signaling, and positively impact glutathione levels [98,99].”
From Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice (2019) · cites this paper as [98]
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- Authors
- Cao, X., Xiao, H., Zhang, Y., Zou, L., Chu, Y., & Chu, X.
- Affiliations
- Jinan University (CN)
- Wuhan Union Hospital (CN)
- Corresponding author
- Chu, Yinghao
- Chu, Xiaofan
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2010-08
- Volume
- 1347
- Pages
- 142-148
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2153259281
- Cited by
- 48
- References
- 38
- Citations by year
- 2024: 5 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 5
- FWCI
- 1.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30971024, 30870845)
- Guangdong Provincial Natural Science Foundation (91510515000009)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- 1,5-dicaffeoylquinic acid, glutathione synthesis, Nrf2, astrocytes, oxygen-glucose deprivation/reoxygenation, cerebral ischemia-reperfusion injury, oxidative stress, gamma-glutamylcysteine synthetase, reactive oxygen species, neuroprotection, antioxidant signaling
- MeSH terms
- Analysis of Variance, Animals, Animals, Newborn, Hypoxia, Astrocytes, Cell Survival, Cells, Cultured, Cerebral Cortex, Glutamate-Cysteine Ligase, Glutathione, Quinic Acid, Transfection, Reperfusion, Reactive Oxygen Species, Oxidative Stress, RNA, Small Interfering, NF-E2-Related Factor 2, Rats
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