Cited by 3Memory and Neural MechanismsMemory Processes and InfluencesNeuroscience and Neuropharmacology Research

Intra-amygdala microinjections of chlorpheniramine impair memory formation or memory retrieval in anxiety- and fear-mediated models

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Serafim, K.R., Russo, P.S.T., Fernandes, C.E.M., Gianlorenço, A.C.L., Mattioli, R.·2016·Brain Research Bulletin

Abstract

H1 receptor histaminergic antagonist, chlorpheniramine (CPA) participates in cognitive performance in various animal models. However, little is known regarding the effects of CPA microinjection into the amygdala on emotional behavior. The purpose of this study was to investigate whether CPA microinjection into the amygdala has the same effect on two models, one anxiety- and the other fear-mediated, in various memory stages using the elevated plus maze (EPM) and the inhibitory avoidance task (IAT) tests. Two experiments were performed with seventy-two adult male Swiss mice. Behavioral testing was performed on two consecutive days, and in both experiments, before each trial, the animals received bilateral microinjections of saline (SAL) or CPA (0.16 nmol). The animals were re-exposed to the EPM or IAT 24h after the first trial. Four experimental groups were tested: SAL-SAL, SAL-CPA, CPA-SAL and CPA-CPA. In experiment 1, a decreased open arm exploration (% open arm entries, %OAE and% open arms time, %OAT) for SAL-SAL and SAL-CPA was showed, while these measures did not decrease for the CPA-SAL and CPA-CPA groups in Trial 2. In experiment 2, an increase of retention latency in relation to training 2 for the groups SAL-SAL and CPA-SAL and a significant decrease in latency for the group SAL-CPA was revealed. These results indicate that chlorpheniramine microinjection into the amygdala impairs emotional memory acquisition and/or consolidation in the EPM and retrieval of IAT.

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Authors
Serafim, K. R., Russo, P. S. T., Fernandes, C. E. M., Gianlorenço, A. C. L., & Mattioli, R.
Affiliations
  • Universidade Federal de São Carlos (BR)
  • Universidade de São Paulo (BR)
Corresponding author
Serafim, K.R.
Venue
Brain Research Bulletin
Publisher
Elsevier BV
Published
2016-07
Volume
125
Pages
127-133
ISSN
0361-9230 (ISSN-L)
OpenAlex
W2469701845
Cited by
3
References
45
Citations by year
2026: 1 · 2022: 1 · 2017: 1
FWCI
0.21
Type
journal article
Language
en
Open access
Locations
  • Brain Research Bulletin (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (2012/10913-0)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (303088/2014-1)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
chlorpheniramine, amygdala, histamine H1 receptor antagonists, anxiety, fear, memory consolidation, memory retrieval, emotional memory, elevated plus maze, passive avoidance, memory impairment
MeSH terms
Amygdala, Animals, Anxiety, Avoidance Learning, Chlorpheniramine, Disease Models, Animal, Fear, Histamine H1 Antagonists, Inhibition, Psychological, Male, Memory Disorders, Microinjections, Mental Recall, Maze Learning, Mice

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