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Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells

Wen, Chuangyu, Chen, Junxiong, Zhang, Di, Wang, Huihui, Che, Jia, Qin, Qiyuan, He, Lu, Cai, Zerong, Lin, Mengmeng, Lou, Qiong, Huang, Lanlan, Chen, Daici, Iwamoto, Aikichi, Ren, Donglin, Wang, Lei, Lan, Ping, Wang, Jianping, Liu, Huanliang, Yang, Xiangling·2016·Cancer Letters
DOI 10.1016/j.canlet.2016.09.007
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Image 1
CC-BY-NC-ND · From the PDF
Fig. 1 PAB inhibits proliferation and induces mitotic arrest in RKO and HCT-15 cells. (A) The proliferation of RKO and HCT-15 cells was measured by real-time cell analysis. Mean ± SD (n ¼ 3). ** P < 0.01, *** P < 0.001, versus control group. (B) The colony formation of RKO and HCT-15 cells was counted after cells were treated with indicated concentrations of PAB for 9 days and then were washed, fi xed, and stained with crystal violet. Mean ± SD (n ¼ 3). * P < 0.05, *** P < 0.001. (C) RKO and HCT-15 cells were stained with PI for DNA contents, followed by fl ow cytometric analysis. Mean ± SD (n ¼ 3). *** P < 0.001. (D) Expression levels of cyclin B1 and p-histone h3 in RKO and HCT-15 were detected by western blot analysis. (For interpretation of the references to color in this fi gure legend, the reader is referred to the web version of this article.)
Fig. 1 PAB inhibits proliferation and induces mitotic arrest in RKO and HCT-15 cells. (A) The proliferation of RKO and HCT-15 cells was measured by real-time cell analysis. Mean ± SD (n ¼ 3). ** P < 0.01, *** P < 0.001, versus control group. (B) The colony formation of RKO and HCT-15 cells was counted after cells were treated with indicated concentrations of PAB for 9 days and then were washed, fi xed, and stained with crystal violet. Mean ± SD (n ¼ 3). * P < 0.05, *** P < 0.001. (C) RKO and HCT-15 cells were stained with PI for DNA contents, followed by fl ow cytometric analysis. Mean ± SD (n ¼ 3). *** P < 0.001. (D) Expression levels of cyclin B1 and p-histone h3 in RKO and HCT-15 were detected by western blot analysis. (For interpretation of the references to color in this fi gure legend, the reader is referred to the web version of this article.)CC-BY-NC-ND · From the PDF
Image 3
CC-BY-NC-ND · From the PDF
Fig. 3 Caspase activation is required for the apoptosis but has no effect in mitotic arrest induced by PAB in CRC cells. (A) RKO and HCT-15 cells were treated with PAB for 48 h and 72 h, respectively, in the absence and presence of 50 m Mpan-caspase inhibitor z-VAD-fmk and then apoptotic cell populations were analyzed by fl ow cytometry. Mean ± SD (n ¼ 3). *** P < 0.001. (B) RKO and HCT-15 cells were treated with PAB for 24 h in the absence and presence of 50 m Mpan-caspase inhibitor z-VAD-fmk and stained with PI for DNA contents, followed by fl ow cytometric analysis.
Fig. 3 Caspase activation is required for the apoptosis but has no effect in mitotic arrest induced by PAB in CRC cells. (A) RKO and HCT-15 cells were treated with PAB for 48 h and 72 h, respectively, in the absence and presence of 50 m Mpan-caspase inhibitor z-VAD-fmk and then apoptotic cell populations were analyzed by fl ow cytometry. Mean ± SD (n ¼ 3). *** P < 0.001. (B) RKO and HCT-15 cells were treated with PAB for 24 h in the absence and presence of 50 m Mpan-caspase inhibitor z-VAD-fmk and stained with PI for DNA contents, followed by fl ow cytometric analysis.CC-BY-NC-ND · From the PDF
Image 5
CC-BY-NC-ND · From the PDF
Fig. 5 CDK1 is involved in the effect of PAB to CRC cells. After 12 h PAB treatment, cells were treated with PAB in the absence and presence of RO-3306 or purvalanol A for 12 h and 36 h. (A) Expressions of p-histone h3 and (B) Expressions of cleaved-PARP and caspase3 were detected by western blot.
Fig. 5 CDK1 is involved in the effect of PAB to CRC cells. After 12 h PAB treatment, cells were treated with PAB in the absence and presence of RO-3306 or purvalanol A for 12 h and 36 h. (A) Expressions of p-histone h3 and (B) Expressions of cleaved-PARP and caspase3 were detected by western blot.CC-BY-NC-ND · From the PDF
Fig. 6 PAB induces proliferation inhibition and mitotic arrest in RKO/5FU-R and HCT-15/5FU-R cells. (A) The viability of RKO/5FU-R and HCT-15/5FU-R cells was measured by MTS assay. (B) The colony formation of cells was counted after PAB-treated RKO/5FU-R and HCT-15/5FU-R cells were washed, fi xed and stained with crystal violet. Mean ± SD (n ¼ 3). *** P < 0.001. (C) RKO/5FU-R and HCT-15/5FU-R cells were stained with PI for DNA contents, followed by fl ow cytometric analysis. Mean ± SD (n ¼ 3). * P < 0.05, ** P < 0.01, *** P < 0.001. (D) Expression levels of cyclin B1 and p-histone h3 in RKO/5FU-R and HCT-15/5FU-R cells were detected by western blot analysis. (For interpretation of the references to color in this fi gure legend, the reader is referred to the web version of this article.)
Fig. 6 PAB induces proliferation inhibition and mitotic arrest in RKO/5FU-R and HCT-15/5FU-R cells. (A) The viability of RKO/5FU-R and HCT-15/5FU-R cells was measured by MTS assay. (B) The colony formation of cells was counted after PAB-treated RKO/5FU-R and HCT-15/5FU-R cells were washed, fi xed and stained with crystal violet. Mean ± SD (n ¼ 3). *** P < 0.001. (C) RKO/5FU-R and HCT-15/5FU-R cells were stained with PI for DNA contents, followed by fl ow cytometric analysis. Mean ± SD (n ¼ 3). * P < 0.05, ** P < 0.01, *** P < 0.001. (D) Expression levels of cyclin B1 and p-histone h3 in RKO/5FU-R and HCT-15/5FU-R cells were detected by western blot analysis. (For interpretation of the references to color in this fi gure legend, the reader is referred to the web version of this article.)CC-BY-NC-ND · From the PDF
Image 8
CC-BY-NC-ND · From the PDF
Image 9
CC-BY-NC-ND · From the PDF
Fig. 9 The schematic diagram of possible molecular mechanism of PAB-induced cytotoxicity in CRC cells.
Fig. 9 The schematic diagram of possible molecular mechanism of PAB-induced cytotoxicity in CRC cells.CC-BY-NC-ND · From the PDF

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Metadata

Authors
Wen, C., Chen, J., Zhang, D., Wang, H., Che, J., Qin, Q., He, L., Cai, Z., Lin, M., Lou, Q., Huang, L., Chen, D., Iwamoto, A., Ren, D., Wang, L., Lan, P., Wang, J., Liu, H., & Yang, X.
Venue
Cancer Letters
Publisher
Elsevier BV
Published
2016-12
Volume
383
Issue
2
Pages
295-308
ISSN
0304-3835
DOI
10.1016/j.canlet.2016.09.007
Cited by
35
References
0
Language
en
Licence
Funders
  • Guangdong Innovative Research Team Program (2009010058)
  • Guangdong Provincial Department of Science and Technology (2012B050500004, 2014B020212016)
  • National Key Clinical Discipline
  • National Natural Science Foundation of China (81301978)
  • Overseas Excellent Professor Project, Ministry of Education, China
  • Japan Ministry of Education, Culture, Sports, Science and Technology

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