Chitin oligosaccharides inhibit oxidative stress in live cells
Abstract
The aim of this research is to identify the cellular antioxidant effects of chitin oligosaccharides (NA-COS; M(w) 229.21-593.12 Da) produced by acidic hydrolysis of crab chitin. The inhibitory effect of NA-COS on myeloperoxidase (MPO) activity in human myeloid cells (HL-60) and oxidation of DNA and protein in mouse macrophages (Raw 264.7) were identified. Furthermore, their direct radical scavenging effect by 2',7'-dichlorofluorescein (DCF) intensity and intracellular glutathione (GSH) level were significantly increased in a time dependent manner, respectively. These results suggest that NA-COS act as a potent antioxidant in live cells.
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ROS and NOS attack biomolecules like DNA, proteins, and membrane lipids, leading to many severe diseases including cancer, coronary arteries disease, obesity, diabetes, ischemic stroke, and Alzheimer disease.
“ROS (reactive oxygen species) and NOS (nitrogen reactive species) attack biomolecules like DNA, proteins, and membrane lipids, leading to many severe diseases including cancer, coronary arteries disease, obesity, diabetes, ischemic stroke, and Alzheimer disease [216].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [216]
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- Authors
- Ngo, D.-N., Kim, M.-M., & Kim, S.-K.
- Affiliations
- Pukyong National University (KR)
- Dong-Eui University (KR)
- Corresponding author
- Kim, Moon-Moo
- Kim, Se-Kwon
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2008-10
- Volume
- 74
- Issue
- 2
- Pages
- 228-234
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2037747961
- Cited by
- 163
- References
- 29
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 3 · 2023: 9 · 2022: 8 · 2021: 17 · 2020: 17 · 2019: 10 · 2018: 8 · 2017: 8 · 2016: 7 · 2015: 11 · 2014: 8 · 2013: 8 · 2012: 15
- FWCI
- 3.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Studies on Chitinases and Chitosanases
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- chitin oligosaccharides, antioxidant activity, oxidative stress, myeloperoxidase inhibition, DNA oxidation, protein oxidation, free radical scavenging, glutathione, acid hydrolysis, HL-60 cells, RAW 264.7 macrophages
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