Cited by 169Polysaccharides Composition and ApplicationsPolysaccharides and Plant Cell WallsAgricultural and Biological Research

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Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

Wu, Hao, Zhu, Junxiang, Diao, Wenchao, Wang, Chengrong·2014·Carbohydrate Polymers

DOI 10.1016/j.carbpol.2014.07.025

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Abstract

An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.

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“…one hand, may not have been conducive to the movement of plant cells to the active site of the enzyme, and on the other hand, it was too excessive compared with the fixed liquid–material ratio, and it accordingly resulted in a reduction in enzymatic hydrolysis and lowered the extraction yield [21].”
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“[14] xperimentally concluded that compared with polysaccharides obtained using the hydrother al meth d (Mw = 1.”
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“This result is not surprising, knowing that the enzymes used here are dedicated to the hydrolysis of polysaccharides (glycosidases) and ultrasound have already been shown to intensify the enzymatic hydrolysis of various biomass for the release of carbohydrates and polysaccharides [40,47,48,50,51,54,59].”
Background
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“This indicates that there is a high degree of correlation between the real and predicted data in the created regression model (Wu et al. 2014).”
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“Weibell’s model is widely applied in the extraction process of active substance from plant materials widely because it is simplicity and applicability [33].”
Background
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“This result may be due to low enzyme activities at higher pH values [22].”
Background
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Background
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“However, the data obtained at 24 h of permeation study was considered and displayed as Q24.”
BackgroundMethodInfluential
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Extraction and characterization of bioactive compounds from eggplant peel using ultrasound – assisted extraction
Horincar, G., de Jos", D., Enachi, E., Stănciuc, N., & Gabriela Râpeanu, B. (2019) · The Annals of the University Dunarea de Jos of Galati Fascicle VI – Food Technology · doi:10.35219/foodtechnology.2019.1.03 · Cited by 22
“…compounds using an ultrasound-assisted process was applied to several types of compounds such as polysaccharides from pumpkin and black currant (Wu et al., 2014, Xu et al., 2015), grape amino acids (Carrera et al., 2015), black tea polyphenols (Both et al., 2014) and papaya seed oil (Samaram…”
Method
RSM-Based Design and Optimization of Transdermal Film of Ondansetron HCl
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Recent advances in the extraction, purification, and antioxidant activities of fruit polysaccharides: A review
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Authors: Wu, H., Zhu, J., Diao, W., & Wang, C.
Affiliations: Qingdao Agricultural University (CN)
Corresponding author: Wang, Chengrong
ORCID: Wu, Hao
Zhu, Junxiang
Wang, Chengrong
Venue: Carbohydrate Polymers
Publisher: Elsevier BV
Published: 2014-11
Volume: 113
Pages: 314-324
ISSN: 0144-8617 (ISSN-L)
DOI: 10.1016/j.carbpol.2014.07.025
OpenAlex: W2072936136
Cited by: 169
References: 60
Citations by year: 2026: 8 · 2025: 6 · 2024: 22 · 2023: 12 · 2022: 21 · 2021: 22 · 2020: 13 · 2019: 15 · 2018: 13 · 2017: 9 · 2016: 15 · 2015: 11 · 2014: 2
FWCI: 17.67
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Carbohydrate Polymers (publishedVersion)
PubMed (publishedVersion)
Funders: Modern Vegetable Industry Technology System of Shandong Province (SDAIT-02-022-12)
Shandong province Higher Educational Science and Technology Program (J14LE11)
Talents of Highlevel Scientific Research Foundation of Qingdao Agriculture University (631207)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords: Cucurbita moschata, pumpkin polysaccharide, ultrasound-assisted enzymatic extraction, antioxidant activity, extraction optimization, mass transfer kinetics, enzymatic extraction, DPPH radical scavenging, reducing power, natural antioxidants
MeSH terms: Antioxidants, Polysaccharides, Sound, Free Radical Scavengers, Cucurbita

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Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

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