Cited by 169Polysaccharides Composition and ApplicationsPolysaccharides and Plant Cell WallsAgricultural and Biological Research

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Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

Wu, Hao, Zhu, Junxiang, Diao, Wenchao, Wang, Chengrong·2014·Carbohydrate Polymers

DOI 10.1016/j.carbpol.2014.07.025

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Abstract

An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.

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Authors: Wu, H., Zhu, J., Diao, W., & Wang, C.
Affiliations: Qingdao Agricultural University (CN)
Corresponding author: Wang, Chengrong
ORCID: Wu, Hao
Zhu, Junxiang
Wang, Chengrong
Venue: Carbohydrate Polymers
Publisher: Elsevier BV
Published: 2014-11
Volume: 113
Pages: 314-324
ISSN: 0144-8617 (ISSN-L)
DOI: 10.1016/j.carbpol.2014.07.025
OpenAlex: W2072936136
Cited by: 169
References: 60
Citations by year: 2026: 8 · 2025: 6 · 2024: 22 · 2023: 12 · 2022: 21 · 2021: 22 · 2020: 13 · 2019: 15 · 2018: 13 · 2017: 9 · 2016: 15 · 2015: 11 · 2014: 2
FWCI: 17.67
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Carbohydrate Polymers (publishedVersion)
PubMed (publishedVersion)
Funders: Modern Vegetable Industry Technology System of Shandong Province (SDAIT-02-022-12)
Shandong province Higher Educational Science and Technology Program (J14LE11)
Talents of Highlevel Scientific Research Foundation of Qingdao Agriculture University (631207)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords: Cucurbita moschata, pumpkin polysaccharide, ultrasound-assisted enzymatic extraction, antioxidant activity, extraction optimization, mass transfer kinetics, enzymatic extraction, DPPH radical scavenging, reducing power, natural antioxidants
MeSH terms: Antioxidants, Polysaccharides, Sound, Free Radical Scavengers, Cucurbita

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Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

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