The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Exopolysaccharides of Lactobacillus reuteri: Their Influence on Adherence of E. Coli to Epithelial Cells and Inflammatory Response
Abstract
The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).
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“High-performance, anion-exchange chromatography coupled with pulsed amperometric detection [8,27,35,53] or gas chromatography (GC) [32,43] are commonly utilized to analyze the monosaccharide composition of EPS.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [32]
Citations
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Role of Lactobacillus reuteri in Human Health and Diseases
Mu, Q., Tavella, V. J., & Luo, X. M. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00757 · Cited by 744
“In addition, EPS synthesized by L. reuteri is able to inhibit E. coli adhesion to porcine epithelial cells in vitro (Ksonzekova et al., 2016).”
BackgroundExopolysaccharides of lactic acid bacteria: Structure, bioactivity and associations: A review
Zhou, Y., Cui, Y., & Qu, X. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.11.093 · Cited by 428
Jurášková, D., Ribeiro, S. C., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11020156 · Cited by 342
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194
“In certain cases, heat treatment is used to improve the EPS recovery from the culture medium as the first step in EPS isolation [27,32,35,58,97,98].”
BackgroundMethodInfluentialAmiri, S., Mokarram, R. R., Khiabani, M. S., Bari, M. R., & Alizadeh, M. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.11.084 · Cited by 160
Metadata
- Authors
- Kšonžeková, P., Bystrický, P., Vlčková, S., Pätoprstý, V., Pulzová, L., Mudroňová, D., Kubašková, T., Csank, T., & Tkáčiková, Ľ.
- Affiliations
- University of Veterinary Medicine in Košice (SK)
- Institute of Chemistry of the Slovak Academy of Sciences (SK)
- Corresponding author
- Tkáčiková, Ľudmila
- ORCID
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 141
- Pages
- 10-19
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2207148956
- Cited by
- 122
- References
- 38
- Citations by year
- 2026: 9 · 2025: 16 · 2024: 22 · 2023: 14 · 2022: 18 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 2
- FWCI
- 10.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- VEGA (1/0834/12)
- Probiotech (26220220152)
- European Fund (OAE010101)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Lactobacillus reuteri, exopolysaccharides, enterotoxigenic Escherichia coli, bacterial adhesion, IPEC-1 cells, intestinal epithelial cells, inflammatory cytokines, IL-1β, interleukin-6, TNF-α, NF-κB, glycosidic linkage, polysaccharide molecular weight, prebiotics
- MeSH terms
- Animals, Bacterial Adhesion, Cell Line, Epithelial Cells, Glucans, Polysaccharides, Bacterial, RNA, Messenger, Swine, Tumor Necrosis Factor-alpha, Interleukin-6, Up-Regulation, NF-kappa B, Limosilactobacillus reuteri, Interleukin-1beta, Enterotoxigenic Escherichia coli
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