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Exopolysaccharides of Lactobacillus reuteri: Their Influence on Adherence of E. Coli to Epithelial Cells and Inflammatory Response
Abstract
The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).
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“High-performance, anion-exchange chromatography coupled with pulsed amperometric detection [8,27,35,53] or gas chromatography (GC) [32,43] are commonly utilized to analyze the monosaccharide composition of EPS.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [32]
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
A simple and rapid method for the permethylation of carbohydrates
Ciucanu, I., & Kerek, F. (1984) · Carbohydrate Research · doi:10.1016/0008-6215(84)85242-8 · Cited by 3,581
Englyst, H. N., & Cummings, J. H. (1984) · The Analyst · doi:10.1039/an9840900937 · Cited by 1,095
Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells
Duhen, T., Geiger, R., Jarrossay, D., Lanzavecchia, A., & Sallusto, F. (2009) · Nature Immunology · doi:10.1038/ni.1767 · Cited by 1,079
Beg, A. A., Finco, T. S., Nantermet, P. V., & Baldwin, A. S. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.6.3301 · Cited by 1,007
New Perspectives for Lactobacilli Exopolysaccharides
Badel, S., Bernardi, T., & Michaud, P. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.011 · Cited by 497
PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples
Wang, R. F., Cao, W. W., & Cerniglia, C. E. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.4.1242-1247.1996 · Cited by 493
Impaired expression of peroxisome proliferator-activated receptor γ in ulcerative colitis
Dubuquoy, L., Jansson, E. Å., Deeb, S. S., Rakotobé, S., Karoui, M., Colombel, J., Auwerx, J. H., Pettersson, S., & Desreumaux, P. (2003) · Gastroenterology · doi:10.1016/s0016-5085(03)00271-3 · Cited by 448
Detection of Mycoplasma in cell cultures
Young, L., Sung, J., Stacey, G., & Masters, J. R. (2010) · Nature Protocols · doi:10.1038/nprot.2010.43 · Cited by 437
Cell Wall Structure and Function in Lactic Acid Bacteria
Chapot-Chartier, M.-P., & Kulakauskas, S. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-s1-s9 · Cited by 410
A PRACTICAL GUIDE TO THE METHYLATION ANALYSIS OF CARBOHYDRATES.
Jansson, P., Kenne, L., Liedgren, H., Lindberg, B., & Lōnngren, J. (1976) · Cited by 295
Ruas‐Madiedo, P., Gueimonde, M., Margollés, A., Reyes‐Gavilán, C. G. D. L., & Salminen, S. (2006) · Journal of Food Protection · doi:10.4315/0362-028x-69.8.2011 · Cited by 244
Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides
Liu, C., Tseng, K., Chiang, S., Lee, B., Hsu, W., & Pan, T. (2011) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4456 · Cited by 230
Capsule Shields the Function of Short Bacterial Adhesins
Schembri, M. A., Dalsgaard, D., & Klemm, P. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.5.1249-1257.2004 · Cited by 217
Pham, P. L., Dupont, I., Roy, D., Lapointe, G., & Cerning, J. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2302-2310.2000 · Cited by 212
Antioxidant Activity of an Exopolysaccharide Purified from Lactococcus lactis subsp. lactis 12
Pan, D., & Mei, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.01.005 · Cited by 211
Arena, A., Maugeri, T. L., Pavone, B., Iannello, D., Gugliandolo, C., & Bisignano, G. (2005) · International Immunopharmacology · doi:10.1016/j.intimp.2005.07.004 · Cited by 207
Biosorption of heavy metals by the exopolysaccharide produced by Paenibacillus jamilae
Morillo, J. A., Ribera, R. G., Quesada, T., Aguilera, J., Alberto Ramos-cormenzana, A., & Monteoliva‐Sánchez, M. (2008) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-008-9800-9 · Cited by 200
van den Berg, D. J. C., Robijn, G. W., Janssen, A., Giuseppin, M. L. F., Vreeker, R., Kamerling, J. P., Vliegenthart, J. F. G., Ledeboer, A. M., & Verrips, C. T. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.8.2840-2844.1995 · Cited by 173
Cholesterol Lowering Activity of Ropy Fermented Milk
NAKAJIMA, H., SUZUKI, Y., & HIROTA, T. (1992) · Journal of Food Science · doi:10.1111/j.1365-2621.1992.tb06848.x · Cited by 170
Roselli, M., Finamore, A., Britti, M. S., Konstantinov, S. R., Smidt, H., de Vos, W. M., & Mengheri, E. (2007) · Journal of Nutrition · doi:10.1093/jn/137.12.2709 · Cited by 169
Hsu, H., Hua, K., Lin, C., Lin, C., Hsu, J., & Wong, C. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.10.5989 · Cited by 159
Microbial Exopolysaccharides - their Role in Microbial Adhesion in Aqueous Systems
Sutherland, I. W. (1982) · CRC Critical Reviews in Microbiology · doi:10.3109/10408418209113562 · Cited by 157
Surayot, U., Wang, J., Seesuriyachan, P., Kuntiya, A., Tabarsa, M., Lee, Y., Kim, J., Park, W., & You, S. (2014) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.05.005 · Cited by 141
Moue, M., Tohno, M., Shimazu, T., Kido, T., Aso, H., Saito, T., & Kitazawa, H. (2007) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2007.11.006 · Cited by 134
Structure and functions of exopolysaccharide produced by gut commensal Lactobacillus reuteri 100-23
Sims, I. M., Frese, S. A., Walter, J., Loach, D. M., Wilson, M., Appleyard, K., Eason, J. R., Livingston, M. K., Baird, M. A., Cook, G. M., et al. (2011) · The ISME Journal · doi:10.1038/ismej.2010.201 · Cited by 126
van Leeuwen, S. S., Kralj, S., van Geel-Schutten, I. H., Gerwig, G. J., Dijkhuizen, L., & Kamerling, J. P. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.01.042 · Cited by 121
Moineau, S., Borkaev, M., Holler, B. J., Walker, S. A., Kondo, J. K., Vedamuthu, E. R., & Vandenbergh, P. A. (1996) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(96)76584-0 · Cited by 118
López, P., Monteserín, D. C., Gueimonde, M., Reyes‐Gavilán, C. G. D. L., Margollés, A., Suárez, A., & Ruas‐Madiedo, P. (2011) · Food Research International · doi:10.1016/j.foodres.2011.11.020 · Cited by 115
Bleau, C., Monges, A., Rashidan, K. K., Laverdure, J., Lacroix, M., Calsteren, M. V., Millette, M., Savard, R., & Lamontagne, L. (2009) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2009.04450.x · Cited by 108
Candela, M., Seibold, G. M., Vitali, B., Lachenmaier, S., Eikmanns, B. J., & Brigidi, P. (2005) · Research in Microbiology · doi:10.1016/j.resmic.2005.04.006 · Cited by 86
Guzmán-Murillo, M. A., & Ascencio, F. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2000.00751.x · Cited by 81
van Leeuwen, S. S., Kralj, S., van Geel-Schutten, I. H., Gerwig, G. J., Dijkhuizen, L., & Kamerling, J. P. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.01.044 · Cited by 69
New method for exopolysaccharide determination in culture broth using stirred ultrafiltration cells
Bergmaier, D., Lacroix, C., Macedo, M. G., & Champagne, C. P. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s002530100764 · Cited by 53
Functional Alteration of Macrophages by a Slime-Forming Lactococcus lactis ssp. cremoris
Kitazawa, H., Nomura, M., Itoh, T., & Yamaguchi, T. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78380-x · Cited by 27
Cytokine mRNA expression in porcine cell lines stimulated by enterotoxigenic Escherichia coli
Pavlová, B., Volf, J., Alexa, P., Rychlı́k, I., Matiašovic, J., & Faldyna, M. (2008) · Veterinary Microbiology · doi:10.1016/j.vetmic.2008.04.024 · Cited by 25
Chytilová, M., Nemcová, R., Gancarčíková, S., Mudroňová, D., & Tkáčiková, Ľ. (2014) · Acta Veterinaria Hungarica · doi:10.1556/avet.2014.024 · Cited by 12
Cited by
Sorted by citations
Role of Lactobacillus reuteri in Human Health and Diseases
Mu, Q., Tavella, V. J., & Luo, X. M. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00757 · Cited by 744
“In addition, EPS synthesized by L. reuteri is able to inhibit E. coli adhesion to porcine epithelial cells in vitro (Ksonzekova et al., 2016).”
BackgroundExopolysaccharides of lactic acid bacteria: Structure, bioactivity and associations: A review
Zhou, Y., Cui, Y., & Qu, X. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.11.093 · Cited by 428
Jurášková, D., Ribeiro, S. C., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11020156 · Cited by 342
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194
“In certain cases, heat treatment is used to improve the EPS recovery from the culture medium as the first step in EPS isolation [27,32,35,58,97,98].”
BackgroundMethodInfluentialAmiri, S., Mokarram, R. R., Khiabani, M. S., Bari, M. R., & Alizadeh, M. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.11.084 · Cited by 160
Metadata
- Authors
- Kšonžeková, P., Bystrický, P., Vlčková, S., Pätoprstý, V., Pulzová, L., Mudroňová, D., Kubašková, T., Csank, T., & Tkáčiková, Ľ.
- Affiliations
- University of Veterinary Medicine in Košice (SK)
- Institute of Chemistry of the Slovak Academy of Sciences (SK)
- Corresponding author
- Tkáčiková, Ľudmila
- ORCID
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 141
- Pages
- 10-19
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2207148956
- Cited by
- 122
- References
- 38
- Citations by year
- 2026: 9 · 2025: 16 · 2024: 22 · 2023: 14 · 2022: 18 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 2
- FWCI
- 10.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- VEGA (1/0834/12)
- Probiotech (26220220152)
- European Fund (OAE010101)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Lactobacillus reuteri, exopolysaccharides, enterotoxigenic Escherichia coli, bacterial adhesion, IPEC-1 cells, intestinal epithelial cells, inflammatory cytokines, IL-1β, interleukin-6, TNF-α, NF-κB, glycosidic linkage, polysaccharide molecular weight, prebiotics
- MeSH terms
- Animals, Bacterial Adhesion, Cell Line, Epithelial Cells, Glucans, Polysaccharides, Bacterial, RNA, Messenger, Swine, Tumor Necrosis Factor-alpha, Interleukin-6, Up-Regulation, NF-kappa B, Limosilactobacillus reuteri, Interleukin-1beta, Enterotoxigenic Escherichia coli
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