Effects of Exposure to Multiple Trace Metals on Biochemical, Histological and Ultrastructural Features of Gills of a Freshwater Fish, Channa punctata Bloch
Abstract
The trace metals are frequently encountered as mixtures of essential and non-essential elements. Therefore, evaluation of their toxic effects individually does not offer a realistic estimate of their impact on biological processes. We studied effects of a mixture of four essential and toxic metals (Cu, Cd, Fe and Ni) on biochemical and morphological characteristics of the gills of a biomarker freshwater fish Channa punctata (Bloch) using environmentally relevant concentrations. Fish were exposed to metal mixture through tank water for 7, 15 and 30 days. Biochemical studies as well as light microscopy (LM) and scanning electron microscopy (SEM) revealed significant metal exposure-induced alterations in gills. Besides ultastructural changes, activities of antioxidant enzymes such catalase (CAT), glutathione S-transferase (GST) and superoxide dismutase (SOD) were significantly altered in the gills of exposed fish. The reduced glutathione (GSH) was significantly (p<0.001) decreased, while lipid peroxidation (LPO) was significantly (p<0.001) increased. The main alterations in general morphology of fish gills included spiking and fusion of secondary lamellae, formation of club-shaped filaments, and vacuolization and necrosis of filament epithelium in the interlamellar regions. SEM studies showed gradual increase of the density and apical surface area of the chloride cells and transformation of the surface structure of the pavement cells. The results of this study indicate adaptive as well a toxic responses in fish gills exposed to mixture of trace metals. Low concentrations of trace metal appear to compromise the antioxidant defense of gills. Lesions in the gill morphology caused by the effect of low concentrations of trace metals could lead to functional alterations and interference with fundamental processes such as maintenance of osmoregulation, gas exchange and xenobiotic metabolism in the exposed fish populations.
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“Lowering the intracellular GSH level and decreasing the GSH-related antioxidant enzyme activities at the same time leads to an oxidative imbalance and induces oxidative processes, resulting in increased cell death (Pandey et al., 2008).”
BackgroundHistopathology biomarker responses in Asian sea bass, Lates calcarifer (Bloch) exposed to copper
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“These results are in accordance with earlier reports.([3,77]) Similarly, a relationship among MT and LPO was shown in the freshwater bivalve, Pyganodon grandis, in response to Cd and Zn exposure.”
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Kidney activity increases in copper exposed goldfish ( Carassius auratus auratus )
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“Our results thus corroborate results of previous studies (Pandey et al. 2008, Monteiro et al. 2010).”
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“Research by Pandey et al. (2008) indicated that lipid oxidation processes occurring in gills could result in impaired respiratory efficiency, severely diminishing the organism’s ability to biotransform xenobiotic substances, and thereby increasing susceptibility to toxic compounds.”
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“…ultrastructural lesions in the gill morphology as observed in the present study could lead to functional alterations and interfere with the fundamental process such as maintenance of osmoregulation and antioxidant defence of gills (Arellano et al., 2001; Pandey et al., 2008; Biagini et al., 2009).”
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- Authors
- Pandey, S., Parvez, S., Ansari, R. A., Ali, M., Kaur, M., Hayat, F., Ahmad, F., & Raisuddin, S.
- Affiliations
- Jamia Hamdard (IN)
- Otto-von-Guericke-Universität Magdeburg (DE)
- Corresponding author
- Raisuddin, Sheikh
- Venue
- Chemico-Biological Interactions
- Publisher
- Elsevier BV
- Published
- 2008-08
- Volume
- 174
- Issue
- 3
- Pages
- 183-192
- ISSN
- 0009-2797 (ISSN-L)
- OpenAlex
- W1990995931
- Cited by
- 281
- References
- 57
- Citations by year
- 2026: 5 · 2025: 13 · 2024: 6 · 2023: 10 · 2022: 12 · 2021: 16 · 2020: 14 · 2019: 16 · 2018: 13 · 2017: 32 · 2016: 37 · 2015: 17 · 2014: 19 · 2013: 20 · 2012: 15
- FWCI
- 8.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemico-Biological Interactions (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University Grants Commission
- Ministry of Environment
- All-India Institute of Medical Sciences
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- copper, cadmium, iron, nickel, Channa punctatus, gill histology, gill ultrastructure, antioxidant enzymes, lipid peroxidation, reduced glutathione, chloride cells, pavement cells, metal toxicity, osmoregulation, gas exchange, biomarkers
- MeSH terms
- Animals, Copper, Epithelial Cells, Ferrous Compounds, Fresh Water, Gills, Glutathione, Microscopy, Electron, Scanning, Necrosis, Nickel, Oxidoreductases, Perciformes, Trace Elements, Water Pollutants, Chemical, Lipid Peroxidation, Toxicity Tests, Cadmium Chloride
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