Cited by 281Environmental Toxicology and EcotoxicologyHeavy Metal Exposure and ToxicityAquaculture disease management and microbiota

Effects of Exposure to Multiple Trace Metals on Biochemical, Histological and Ultrastructural Features of Gills of a Freshwater Fish, Channa punctata Bloch

Abstract

The trace metals are frequently encountered as mixtures of essential and non-essential elements. Therefore, evaluation of their toxic effects individually does not offer a realistic estimate of their impact on biological processes. We studied effects of a mixture of four essential and toxic metals (Cu, Cd, Fe and Ni) on biochemical and morphological characteristics of the gills of a biomarker freshwater fish Channa punctata (Bloch) using environmentally relevant concentrations. Fish were exposed to metal mixture through tank water for 7, 15 and 30 days. Biochemical studies as well as light microscopy (LM) and scanning electron microscopy (SEM) revealed significant metal exposure-induced alterations in gills. Besides ultastructural changes, activities of antioxidant enzymes such catalase (CAT), glutathione S-transferase (GST) and superoxide dismutase (SOD) were significantly altered in the gills of exposed fish. The reduced glutathione (GSH) was significantly (p<0.001) decreased, while lipid peroxidation (LPO) was significantly (p<0.001) increased. The main alterations in general morphology of fish gills included spiking and fusion of secondary lamellae, formation of club-shaped filaments, and vacuolization and necrosis of filament epithelium in the interlamellar regions. SEM studies showed gradual increase of the density and apical surface area of the chloride cells and transformation of the surface structure of the pavement cells. The results of this study indicate adaptive as well a toxic responses in fish gills exposed to mixture of trace metals. Low concentrations of trace metal appear to compromise the antioxidant defense of gills. Lesions in the gill morphology caused by the effect of low concentrations of trace metals could lead to functional alterations and interference with fundamental processes such as maintenance of osmoregulation, gas exchange and xenobiotic metabolism in the exposed fish populations.

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Crossref · 10.1016/j.cbi.2008.05.014

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  • MPs exposure causes metabolic disorders in experimental conditions.

    “Some authors demonstrated that MPs’ exposure causes metabolic disorders in experimental conditions [68,77].”

    From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [68]

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Authors
Pandey, S., Parvez, S., Ansari, R. A., Ali, M., Kaur, M., Hayat, F., Ahmad, F., & Raisuddin, S.
Affiliations
  • Jamia Hamdard (IN)
  • Otto-von-Guericke-Universität Magdeburg (DE)
Corresponding author
Raisuddin, Sheikh
Venue
Chemico-Biological Interactions
Publisher
Elsevier BV
Published
2008-08
Volume
174
Issue
3
Pages
183-192
ISSN
0009-2797 (ISSN-L)
OpenAlex
W1990995931
Cited by
281
References
57
Citations by year
2026: 5 · 2025: 13 · 2024: 6 · 2023: 10 · 2022: 12 · 2021: 16 · 2020: 14 · 2019: 16 · 2018: 13 · 2017: 32 · 2016: 37 · 2015: 17 · 2014: 19 · 2013: 20 · 2012: 15
FWCI
8.04
Type
journal article
Language
en
Open access
closed
Locations
  • Chemico-Biological Interactions (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University Grants Commission
  • Ministry of Environment
  • All-India Institute of Medical Sciences
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
Keywords
copper, cadmium, iron, nickel, Channa punctatus, gill histology, gill ultrastructure, antioxidant enzymes, lipid peroxidation, reduced glutathione, chloride cells, pavement cells, metal toxicity, osmoregulation, gas exchange, biomarkers
MeSH terms
Animals, Copper, Epithelial Cells, Ferrous Compounds, Fresh Water, Gills, Glutathione, Microscopy, Electron, Scanning, Necrosis, Nickel, Oxidoreductases, Perciformes, Trace Elements, Water Pollutants, Chemical, Lipid Peroxidation, Toxicity Tests, Cadmium Chloride

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