High-throughput analysis of signals regulating stem cell fate and function
Open accessAbstract
Stem cells exhibit promise in numerous areas of regenerative medicine. Their fate and function are governed by a combination of intrinsic determinants and signals from the local microenvironment, or niche. An understanding of the mechanisms underlying both embryonic and adult stem cell functions has been greatly enhanced by the recent development of several high-throughput technologies: microfabricated platforms, including cellular microarrays, to investigate the combinatorial effects of microenvironmental stimuli and large-scale screens utilizing small molecules and short interfering RNAs to identify crucial genetic and signaling elements. Furthermore, the integration of these systems with other versatile platforms, such as microfluidics and lentiviral microarrays, will continue to enable the detailed elucidation of stem cell processes, and thus, greatly contribute to the development of stem cell based therapies.
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Results from 2D and 3D spheroid culture indicated spheroids were more resistant to 5-fluorouracil, whereas 2D cultures were more resistant to tirapazamine.
“Their results from 2D and 3D spheroid culture indicated spheroids were more resistant to one anti-cancer drug, 5-fluorouracil, which inhibits proliferation, whereas 2D cultures were more resistant to a different anticancer drug, tirapazamine, which is cytotoxic in hypoxic conditions (111).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [111]
The Introduction discusses stem-cell culture platforms and small-molecule screens, but does not report a 2D-versus-spheroid drug-response comparison. Searches for spheroids, 5-fluorouracil, and tirapazamine found no passage addressing the claimed resistance results; the indexed passages provide no page numbers. (LLM, conf=0.76)
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- Authors
- Underhill, G. H., & Bhatia, S. N.
- Affiliations
- Harvard–MIT Division of Health Sciences and Technology (US)
- Brigham and Women's Hospital (US)
- Corresponding author
- Bhatia, Sangeeta N
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 2007-08
- Volume
- 11
- Issue
- 4
- Pages
- 357-366
- ISSN
- 1367-5931 (ISSN-L)
- PMC
- PMC2824500
- OpenAlex
- W1978637719
- Cited by
- 93
- References
- 71
- Citations by year
- 2025: 2 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 7 · 2012: 14
- FWCI
- 0.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Current Opinion in Chemical Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Foundation
- David and Lucile Packard Foundation
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK056966, R01DK065152)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- stem cell fate, stem cell function, stem cell niche, high-throughput screening, microfluidics, small molecule screening, embryonic stem cells, adult stem cells, stem cell therapy
- MeSH terms
- Animals, Cell Differentiation, Humans, Stem Cells, Signal Transduction, Cell Culture Techniques, Protein Array Analysis
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