Artificial niche microarrays for probing single stem cell fate in high throughput
Open accessAbstract
To understand the regulatory role of niches in maintaining stem-cell fate, multifactorial in vitro models are required. These systems should enable analysis of biochemical and biophysical niche effectors in a combinatorial fashion and in the context of a physiologically relevant cell-culture substrate. We report a microengineered platform comprised of soft hydrogel microwell arrays with modular stiffness (shear moduli of 1-50 kPa) in which individual microwells can be functionalized with combinations of proteins spotted by robotic technology. To validate the platform, we tested the effect of cell-cell interactions on adipogenic differentiation of adherent human mesenchymal stem cells (MSCs) and the effect of substrate stiffness on osteogenic MSC differentiation. We also identified artificial niches supporting extensive self-renewal of nonadherent mouse neural stem cells (NSCs). Using this method, it is possible to probe the effect of key microenvironmental perturbations on the fate of any stem cell type in single cells and in high throughput.
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Synthetic polymer-based culture allows organoid formation conditions to be evaluated using high-throughput methods.
“Synthetic polymer-based culture allows organoid formation conditions to be evaluated using high-throughput methods143–145.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [144]
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Synthetic 3D PEG-Anisogel Tailored with Fibronectin Fragments Induce Aligned Nerve Extension
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Self-citationHigh-Throughput Routes to Biomaterials Discovery
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Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures
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Self-citation
- Authors
- Gobaa, S., Hoehnel, S., Roccio, M., Negro, A., Kobel, S., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Venue
- Nature Methods
- Published
- 2011-10-09
- Volume
- 8
- Issue
- 11
- Pages
- 949-955
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2061218357
- Cited by
- 390
- References
- 29
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 5 · 2023: 5 · 2022: 11 · 2021: 28 · 2020: 22 · 2019: 19 · 2018: 35 · 2017: 46 · 2016: 40 · 2015: 27 · 2014: 51 · 2013: 56 · 2012: 35
- FWCI
- 22.39
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- stem cell niche, substrate stiffness, mesenchymal stem cells, adipogenesis, osteogenic differentiation, neural stem cells, self-renewal, cell-cell interactions, high-throughput single-cell analysis
- MeSH terms
- Animals, Cell Differentiation, Cell Lineage, Hydrogels, Mice, Mesenchymal Stem Cells
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