Cited by 390Cellular Mechanics and Interactions3D Printing in Biomedical ResearchHydrogels: synthesis, properties, applications

Artificial niche microarrays for probing single stem cell fate in high throughput

Open access
Gobaa, Samy, Hoehnel, Sylke, Roccio, Marta, Negro, Andrea, Kobel, Stefan, Lutolf, Matthias P·2011·Nature Methods

Abstract

To understand the regulatory role of niches in maintaining stem-cell fate, multifactorial in vitro models are required. These systems should enable analysis of biochemical and biophysical niche effectors in a combinatorial fashion and in the context of a physiologically relevant cell-culture substrate. We report a microengineered platform comprised of soft hydrogel microwell arrays with modular stiffness (shear moduli of 1-50 kPa) in which individual microwells can be functionalized with combinations of proteins spotted by robotic technology. To validate the platform, we tested the effect of cell-cell interactions on adipogenic differentiation of adherent human mesenchymal stem cells (MSCs) and the effect of substrate stiffness on osteogenic MSC differentiation. We also identified artificial niches supporting extensive self-renewal of nonadherent mouse neural stem cells (NSCs). Using this method, it is possible to probe the effect of key microenvironmental perturbations on the fate of any stem cell type in single cells and in high throughput.

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Crossref · 10.1038/nmeth.1732

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  • Synthetic polymer-based culture allows organoid formation conditions to be evaluated using high-throughput methods.

    “Synthetic polymer-based culture allows organoid formation conditions to be evaluated using high-throughput methods143–145.”

    From Towards organoid culture without Matrigel (2021) · cites this paper as [144]

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Authors
Gobaa, S., Hoehnel, S., Roccio, M., Negro, A., Kobel, S., & Lutolf, M. P.
Affiliations
École Polytechnique Fédérale de Lausanne (CH)
Venue
Nature Methods
Published
2011-10-09
Volume
8
Issue
11
Pages
949-955
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2061218357
Cited by
390
References
29
Citations by year
2026: 1 · 2025: 5 · 2024: 5 · 2023: 5 · 2022: 11 · 2021: 28 · 2020: 22 · 2019: 19 · 2018: 35 · 2017: 46 · 2016: 40 · 2015: 27 · 2014: 51 · 2013: 56 · 2012: 35
FWCI
22.39
Type
journal article
Language
en
Open access
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
stem cell niche, substrate stiffness, mesenchymal stem cells, adipogenesis, osteogenic differentiation, neural stem cells, self-renewal, cell-cell interactions, high-throughput single-cell analysis
MeSH terms
Animals, Cell Differentiation, Cell Lineage, Hydrogels, Mice, Mesenchymal Stem Cells

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