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Cited by 217Molecular Sensors and Ion DetectionAdvanced Fluorescence Microscopy TechniquesTrace Elements in Health

Imaging mobile zinc in biology

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Tomat, Elisa, Lippard, Stephen J·2010·Current Opinion in Chemical Biology
DOI 10.1016/j.cbpa.2009.12.010PMC PMC2847655
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Abstract

Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence emission properties have provided sensitive tools for mobile zinc imaging, and fluorescence microscopy experiments have afforded depictions of zinc distribution within live cells and tissues. Both small-molecule and protein-based fluorescent probes can address complex imaging challenges, such as analyte quantification, site-specific sensor localization, and real-time detection.

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  • The presence of Zn2+ causes a significant enhancement of the emission intensity in 2 and 4, similar to reported Zn fluorescent sensors containing N-(2-pyridylmethyl) arms.

    “However, the presence of Zn2+ causes a significant enhancement of the emission intensity in 2 and 4, (Figures S17 and S18), similar to the reported Zn fluorescent sensors containing N-(2-pyridylmethyl) arms.73,74”

    From Bifunctional Compounds for Controlling Metal-Mediated Aggregation of the Aβ<sub>42</sub>Peptide (2012) · cites this paper as [74]

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Authors
Tomat, E., & Lippard, S. J.
Affiliations
Massachusetts Institute of Technology (US)
Corresponding author
Lippard, Stephen J
ORCID
Venue
Current Opinion in Chemical Biology
Publisher
Elsevier BV
Published
2010-04
Volume
14
Issue
2
Pages
225-230
ISSN
1367-5931 (ISSN-L)
DOI
10.1016/j.cbpa.2009.12.010
PMC
PMC2847655
OpenAlex
W1567645807
Cited by
217
References
0
Citations by year
2026: 3 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 10 · 2018: 12 · 2017: 11 · 2016: 14 · 2015: 14 · 2014: 31 · 2013: 32 · 2012: 34
FWCI
2.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Opinion in Chemical Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
Funders
  • National Institutes of Health (5r01gm065519-16)
  • National Institute of General Medical Sciences (R01‐GM065519, GM065519)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
Keywords
zinc imaging, zinc regulation, fluorescent probes, live-cell imaging, chemical probes, real-time detection
MeSH terms
Animals, Fluorescent Dyes, Humans, Microscopy, Fluorescence, Spectrometry, Fluorescence, Zinc, Biosensing Techniques

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