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Cited by 39Hormonal and reproductive studiesAdrenal Hormones and DisordersClinical Laboratory Practices and Quality Control

Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC–MS/MS and associations to health markers in 304 men

Damgaard–Olesen, A., Johannsen, T.H., Holmboe, S.A., Søeborg, T., Petersen, J.H., Andersson, AM., Aadahl, M., Linneberg, A. +1 more·2016·Clinica Chimica Acta
DOI 10.1016/j.cca.2015.12.042
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Abstract

We report reference ranges based on LC-MS/MS for testosterone (T), free testosterone (FT) and its precursors, i.e. 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEAS) and androstenedione (Adione), in relation to different health markers and lifestyle factors. The study was based on 304 healthy men aged 30-61 years participating in a population-based cross-sectional study (Health2008). Examination program consisted of a clinical examination, completion of a self-administered questionnaire and blood sampling. Steroid metabolites were measured by a validated and sensitive LC-MS/MS method. Older age-groups were significantly associated with decreased concentrations of DHEA, DHEAS, Adione, and FT, while no significant associations with age were shown for 17-OHP or T. Participants with BMI≥30 kg/m(2) had lower age-related steroid metabolite z-scores compared to participants with BMI<30 kg/m(2), i.e. 17-OHP: -0.51 vs. 0.08 (p<0.001); DHEA: -0.27 vs. 0.09 (p=0.014); Adione: -0.29 vs. 0.09 (p=0.012); T: -0.99 vs. 0.14 (p<0.001); and FT -0.55 vs. 0.05 (p<0.001), respectively. In conclusion, this large study on serum steroid metabolites and concomitant assessment of health markers in healthy men provides age-related reference ranges, and furthermore evaluates the impact of lifestyle factors and metabolic syndrome on androgen metabolite levels.

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  • Free T concentrations usually range around 1-2% of total T and are also age-dependent in women and men.

    “Free T concentrations usually range around 1–2% of total T and are also age-dependent in women (Haring et al., 2012) and men (Camacho et al., 2013, Damgaard-Olesen et al., 2016).”

    From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Damgaard-Olesen et al., 2016

  • In both men and women, ageing leads to decreases in C19 steroid production.

    “In both men and women, ageing leads to decreases in C19 steroid production (Damgaard-Olesen et al., 2016, Haring et al., 2012, Trabert et al., 2016).”

    From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Damgaard-Olesen et al., 2016

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Authors
Damgaard–Olesen, A., Johannsen, T. H., Holmboe, S. A., Søeborg, T., Petersen, J. H., Andersson, A., Aadahl, M., Linneberg, A., & Juul, A.
Affiliations
University of Copenhagen (DK)
Corresponding author
Juul, A.
ORCID
Venue
Clinica Chimica Acta
Publisher
Elsevier BV
Published
2016-02
Volume
454
Pages
82-88
ISSN
0009-8981 (ISSN-L)
DOI
10.1016/j.cca.2015.12.042
OpenAlex
W2227011085
Cited by
39
References
40
Citations by year
2024: 3 · 2023: 2 · 2022: 8 · 2021: 4 · 2020: 5 · 2019: 3 · 2018: 5 · 2017: 7 · 2016: 2
FWCI
5.69
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Clinica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
  • Anita and Tage Therkelsen Foundation (F-22717-16)
  • Kirsten and Freddy Johansens Foundation (F-22181-03)
  • ReproHigh (F-22717-07)
  • ReproUnion (F-22717-26)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Keywords
reference values, 17-hydroxyprogesterone, dehydroepiandrosterone, DHEA sulfate, androstenedione, free testosterone, total testosterone, androgen precursors, body mass index, metabolic syndrome, lifestyle factors, healthy men
MeSH terms
Adult, Androstenedione, Blood Chemical Analysis, Chromatography, Liquid, Cross-Sectional Studies, Dehydroepiandrosterone, Humans, Male, Middle Aged, Sulfates, Testosterone, Biomarkers, 17-alpha-Hydroxyprogesterone, Tandem Mass Spectrometry, Healthy Volunteers

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