The DOI matches its Crossref record; 45 of the 47 references it cites were found in a registry or an index; 1 of the 7 sources its claims cite were checked for support.
Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-α prolyl hydroxylase
Open accessAbstract
Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-α prolyl hydroxylase
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TL;DR
In HEK293 cells, knocking down the SDHD subunit of succinate dehydrogenase caused succinate to accumulate and inhibited HIF-α prolyl hydroxylases, stabilizing HIF-1α under normoxic conditions. This provides a mechanism linking TCA cycle dysfunction to vascular tumor phenotypes without VHL mutation.
Key findings
- SDHD-targeting siRNA reduced SDH activity and raised cellular succinate from about 121 µM in controls to about 447 µM (Di3) and 441 µM (Di4); no significant change was seen in DHE oxidation or glutathione redox state, arguing against ROS as the mediator.
- SDH inhibition induced HIF-1α protein under normoxia, produced about a 3-fold increase in HRE-luciferase reporter activity, induced VEGF mRNA to levels comparable with hypoxia or CoCl₂, and increased BNIP3; HIF-2α induction was less pronounced.
- Succinate inhibited PHD activity in vitro in a dose-dependent manner, with an IC50 reported as 0.5 mM, and the membrane-permeable succinate derivative DMS (20 mM, 48 hr) elevated HIF-1α and caused nuclear HIF-1α accumulation under normoxia.
- In SDH-inhibited cells, pVHL binding to the ODD domain was reduced, consistent with decreased ODD hydroxylation; the proposed model is that mitochondrial succinate exits to the cytosol, inhibits PHD, stabilizes HIF-1α, and drives angiogenesis, metastasis, and glycolytic gene expression.
Methods
- HEK293 cells were transfected with scrambled siRNA or SDHD-targeting siRNA (Di3 or Di4); SDH activity was measured by succinate-DCIP oxidoreductase, succinate by GC-MS with deuterated succinic acid as reference, and ROS/redox state by DHE staining and glutathione assays.
- HIF readouts included Western blot for HIF-1α, HIF-2α, and BNIP3, RT-PCR for VEGF, and an HRE-luciferase reporter; PHD activity was assayed in vitro using HA-tagged ODD and cell extracts, and pVHL binding was assessed by immunoprecipitation and far Western blot.
- The study is cell-culture based, with no patient tumor samples or in vivo tumor models described.
Limitations
- Stated in the paper: DMS uptake, conversion to succinate, and metabolism are unknown; different cell types may have different thresholds and mechanisms of HIF activation; succinate may also modulate FIH; and the ROS findings conflict with C. elegans mev-1 observations, possibly due to species or mutation differences.
- Evident from the text: conclusions rely on HEK293 cells and siRNA knockdown rather than SDH-mutant tumors, transfection efficiency was about 50% so populations were mixed, and no in vivo tumor growth or patient tumor validation was performed; elevated succinate in human SDH-mutant tumors is cited as personal communication.
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In the paper
“Since pVHL binding to the ODD domain is dependent on PHD activity (Kaelin, 2002;Safran and Kaelin, 2003)”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Elevation of succinate level following downregulation of SDH leads to PHD inhibition and HIF-1␣ stabilization in cells
What the sources say
- Not checked — no full text of the sourcecites Kaelin, 2002 source
- Supportedcites Safran and Kaelin, 2003 source
The section “Prolyl hydroxylation earmarks HIF for destruction” (p. 2) says pVHL binds HIF1α only after prolyl residues in its ODD are enzymatically hydroxylated, and identifies PHD proteins as the hydroxylases. The Figure 1 discussion (p. 3) adds that when PHD cannot hydroxylate HIF, pVHL does not recognize it. (LLM, conf=0.94)
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesThe paper states this without a source or data behind it here, so there is nothing to check it against. The quote shows it is in the paper.
In the paper
“succinate, which accumulates as a result of SDH inhibition, inhibits HIF-␣ prolyl hydroxylases in the cytosol”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Abstract
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“cells incubated with 20 mM DMS for 48 hr showed elevated levels of HIF-1␣ under normoxic conditions (Figure 5B).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Elevation of succinate level following downregulation of SDH leads to PHD inhibition and HIF-1␣ stabilization in cells
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition”
- Also stated in: § Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition · plain
“there was no detectable difference in DHE oxidation in Di3 and Di4-transfected cells relative to the control (compare Figures 3C and 3D to 3A).”
- Also stated in: § Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition · plain
“no significant difference was observed in the redox state of SDH-inhibited cells as compared to the control scRNAi-transfected cells (Table 1).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passes- Also stated in: § Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition · plain
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In Di3-transfected cells, even though the total GFP-ODD level was higher than in scRNAitransfected cells (Figures 6Aiv and 6Av), the levels of pVHLassociated fluorescence were significantly lower (Figure 6C).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Elevation of succinate level following downregulation of SDH leads to PHD inhibition and HIF-1␣ stabilization in cells
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In this paper, we show that downregulation of SDH activity results in the accumulation of succinate in cells”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Introduction
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“a clear accumulation of HIF-1␣ in the nuclei of cells treated with DMS was observed (Figure 5C)”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Elevation of succinate level following downregulation of SDH leads to PHD inhibition and HIF-1␣ stabilization in cells
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“no significant difference was observed in the redox state of SDH-inhibited cells as compared to the control scRNAi-transfected cells (Table 1).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“there was no detectable difference in DHE oxidation in Di3 and Di4-transfected cells relative to the control (compare Figures 3C and 3D to 3A).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Reactive oxygen species do not play a role in HIF-1␣ induction after SDH inhibition
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“succinate, which accumulates as a result of SDH inhibition, inhibits HIF-␣ prolyl hydroxylases in the cytosol”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Abstract
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passes- Rests on an assumption: Succinate moves freely between the mitochondria and the cytosol via the dicarboxylic acid translocator in the mitochondrial inner membrane and the voltage-dependent anion channel (VDAC/porin) in the mitochondrial outer membrane.
- Rests on an assumption: Succinate is the substrate for SDH in mitochondria and a product of PHD in the cytosol.
- It may state two separate claims as one. 3
- The quote only partly states this claim. 1
- Probably not a substantial claim. 2
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The IC 50 was 5.0ف mM for succinate under these reaction conditions, a level that is attainable in SDH-inhibited cells (Table 1).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Succinate is accumulated in SDH-inhibited cells
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes? Based: unclearIt may state two separate claims as one.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“leading to stabilization and activation of HIF-1␣.”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Abstract
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesThe quote only partly states this claim.
- Rests on an assumption: Succinate moves freely between the mitochondria and the cytosol via the dicarboxylic acid translocator in the mitochondrial inner membrane and the voltage-dependent anion channel (VDAC/porin) in the mitochondrial outer membrane.
A claim is checked only against the cited paper’s full text, never its abstract, and none is available for its sources.
In the paper
“succinate has been shown to inhibit some ␣-ketoglutarate-dependent oxygenases, including procollagen prolyl hydroxylase and thymine-7-hydroxylase (Holme, 1975;Myllyla et al., 1977).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Introduction
What the sources say
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesProbably not a substantial claim.
What the paper did — it cites no source for this, so there is none to check. The quote shows it is in the paper.
In the paper
“Actin was used as loading control.”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Experimental procedures Succinic acid quantification by GCMS Plasmids
Strength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✗ Load-bearing: fails✓ Based: passesProbably not a substantial claim. The paper’s conclusions may not depend on it.
In the paper
“The PHD isozymes belong to the Fe 2ϩ and ␣-ketoglutarate-dependent family of dioxygenases that split molecular oxygen to hydroxylate their substrates and, in parallel, oxidize and decarboxylate ␣-ketoglutarate to succinate (Schofield and Zhang, 1999).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Introduction
What the sources say
- Not checked — no full text of the sourcecites Schofield and Zhang, 1999 source
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesIt may state two separate claims as one.
A claim is checked only against the cited paper’s full text, never its abstract, and none is available for its sources.
In the paper
“the PHDs utilize ␣-ketoglutarate as a cosubstrate and require ferrous iron (Fe 2ϩ ) and ascorbate as cofactors (Kaelin, 2002;Schofield and Zhang, 1999).”
§ Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-␣ prolyl hydroxylase > Introduction
What the sources say
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesIt may state two separate claims as one.
The paper relies on this without support.
Succinate moves freely between the mitochondria and the cytosol via the dicarboxylic acid translocator in the mitochondrial inner membrane and the voltage-dependent anion channel (VDAC/porin) in the mitochondrial outer membrane.
“Succinate moves freely between the mitochondria and the cytosol via the dicarboxylic acid translocator in the mitochondrial inner membrane and the voltage-dependent anion channel (VDAC/porin) in the mitochondrial outer membrane.”
The paper relies on this without support.
Succinate is the substrate for SDH in mitochondria and a product of PHD in the cytosol.
“succinate, the substrate for SDH in mitochondria and a product of PHD in the cytosol.”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In intact cells, this introduces an additional level of complexity, because the lack of enzymatically active CII will result in the accumulation of its substrate succinate due to the poor membrane permeability of this metabolite.27”
From Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation (2015) · cites this paper as [27]
Experimental — accuracy not yet measuredIn the citing paper
“Our data provide in vivo evidence to support the hypothesis that increased succinate and/or fumarate causes stabilization of HIF1a”
From Accumulation of Krebs cycle intermediates and over-expression of HIF1α in tumours which result from germline FH and SDH mutations (2005) · cites this paper as [27]
In the citing paper
“Selak et al. (27) tested a model of tumorigenesis in which raised levels of succinate caused increased HIF1a expression in vitro.”
From Accumulation of Krebs cycle intermediates and over-expression of HIF1α in tumours which result from germline FH and SDH mutations (2005) · cites this paper as [27]
In the citing paper
“neither our data nor those of Selak et al. (27) have provided evidence for an alternative model of tumorigenesis in HLRCC or HPGL based on increased levels of ROSs.”
From Accumulation of Krebs cycle intermediates and over-expression of HIF1α in tumours which result from germline FH and SDH mutations (2005) · cites this paper as [27]
In the citing paper
“Selak et al. (27) showed that the mechanism for these findings may be inhibition by succinate of HIF PHDs, which rely for their activity on conversion of a-ketoglutarate to succinate.”
From Accumulation of Krebs cycle intermediates and over-expression of HIF1α in tumours which result from germline FH and SDH mutations (2005) · cites this paper as [27]
In the citing paper
“Additionally, succinate and fumarate inhibit the prolyl hydroxylase domain-containing (PHD) enzymes that regulate stability of hypoxia-inducible factors (HIFs), such that FH and SDH deficient cancers activate the hypoxic gene expression program even under normoxic conditions (Hewitson et al., 2007; Pollard et al., 2005; Selak et al., 2005).”
From Targeting Metabolism for Cancer Therapy (2017) · cites this paper as Selak et al., 2005
In the citing paper
“Metabolite accumulation in the setting of dysfunctional mitochondria can inhibit VHL-dependent degradation of hypoxia-inducible factor-α (HIF-α) subunits, creating a pseudohypoxic state that mimics VHL loss (14).”
From Metabolic reprogramming and cancer progression (2020) · cites this paper as [14]
In the citing paper
“The other mechanism by which succinate supports tumorigenesis acts through the inhibition of hypoxia-inducible factor prolyl hydroxylase (PHD). PHD activates the pseudohypoxic response by stabilizing HIF-1α, which is a well-known tumorigenesis enhancer”
From Glutamine reliance in cell metabolism (2020) · cites this paper as [127]
In the citing paper
“TCA cycle dysfunction leads to oncogenesis by regulating signaling pathway and stabilizing HIF1-α (Selak et al., 2005).”
From Tumor microenvironment derived exosomes pleiotropically modulate cancer cell metabolism (2016) · cites this paper as Selak et al., 2005
In the citing paper
“Dysregulated SDH activity in the setting of these mutations promotes the accumulation of succinate and mitochondrial reactive oxygen species, activating HIF1α and inducing angiogenesis (Selak et al., 2005).”
From SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways (2013) · cites this paper as Selak et al., 2005
In the citing paper
“H2O2 also can participate in the one-electron reaction with transition metal ions (e.g., Fe2+ bound to organic molecules) to generate various intermediate ferryl species (274, 275), such as Fe(IV) = O•, a powerful oxidizing agent that can lead to lipid oxidation and DNA damage, as discussed in section VI.”
From Mitochondrial reactive oxygen species production in excitable cells: Modulators of mitochondrial and cell function (2009) · cites this paper as [275]
In the citing paper
“High levels of succinate resulting from loss of complex II function can in turn block HIF1α degradation through inhibition of prolyl hydroxylases.”
From A HIF1α Regulatory Loop Links Hypoxia and Mitochondrial Signals in Pheochromocytomas (2005) · cites this paper as [19]
In the citing paper
“Selak et al. showed that disruption of the mitochondrial complex II results in increased HIF1α activity and that this upregulation is channeled through inhibition of prolyl hydroxylase function [19].”
From A HIF1α Regulatory Loop Links Hypoxia and Mitochondrial Signals in Pheochromocytomas (2005) · cites this paper as [19]
In the citing paper
“Succinate activates HIF-1α in tumours10,26, indicating an important similarity between inflammation and cancer.”
From Succinate is an inflammatory signal that induces IL-1β through HIF-1α (2013) · cites this paper as [10]
In the citing paper
“Succinate can be transported from the mitochondria via the dicarboxylic acid transporter to the cytosol where in excess it impairs PHD activity (by product inhibition) leading to HIF-1α stabilization and activation.”
From Succinate is an inflammatory signal that induces IL-1β through HIF-1α (2013) · cites this paper as [10]
In the citing paper
“Selak et al. reported that complex II deficiency achieved by the suppression of SdhD induces normoxic HIF-1 𝛂 stabilization via the accumulation of succinate without inducing a detectable increase in ROS production (43, 44).”
From Loss of the SdhB, but Not the SdhA, Subunit of Complex II Triggers Reactive Oxygen Species-Dependent Hypoxia-Inducible Factor Activation and Tumorigenesis (2008) · cites this paper as [43]
In the citing paper
“Decreases in Sdh activity should cause an accumulation of succinate, which can transit to the cytosol through the dicarboxylate carrier. There, it potentially can inhibit PHD and cause HIFα stabilization through a pseudohypoxia pathway that does not require ROS (43, 44).”
From Loss of the SdhB, but Not the SdhA, Subunit of Complex II Triggers Reactive Oxygen Species-Dependent Hypoxia-Inducible Factor Activation and Tumorigenesis (2008) · cites this paper as [43]
In the citing paper
“We agree that increases in succinate represent an important contribution to the stabilization of HIFα in cells with Sdh inhibition (43).”
From Loss of the SdhB, but Not the SdhA, Subunit of Complex II Triggers Reactive Oxygen Species-Dependent Hypoxia-Inducible Factor Activation and Tumorigenesis (2008) · cites this paper as [43]
In the citing paper
“PHDs are inactive at low O2 and can be inactivated by the accumulation of TCA metabolites fumarate and succinate, which may occur from mutations in the tumor suppressor genes succinate dehydrogenase subunit B or fumarate hydratase (187, 334, 384).”
From Redox regulation of mitochondrial function (2012) · cites this paper as [384]
In the citing paper
“Loss-of-function mutations in SDH and FH cause a build-up of succinate and fumarate, respectively87. Accordingly, increased levels of succinate and fumarate caused by mutations in these genes can compete with αKG and inhibit activity of HIF hydroxylases and thus induce normoxic HIF1α activity in vitro88,89.”
From Targeting cancer vulnerabilities with high-dose vitamin C (2019) · cites this paper as [88]
In the citing paper
“In addition to affecting the activity of chromatin-associated αKG-dependent dioxygenases, succinate and fumarate accumulation can also inhibit αKG-dependent prolyl hydroxylases, leading to stabilization of HIF-1 and concomitant activation of the HIF/hypoxia transcriptional program (184, 185).”
From Regulation and function of the mammalian tricarboxylic acid cycle (2023) · cites this paper as [185]
In the citing paper
“However, succinate is also an inhibitor of HIF-1α(213), which has been implicated in the exaggerated production of sFlt-1 and in the pathophysiology of preeclampsia(214, 215)”
From Metformin, the Aspirin of the 21st Century: Its Role in Gestational Diabetes Mellitus, Prevention of Preeclampsia and Cancer, and the Promotion of Longevity (2017) · cites this paper as [213]
In the citing paper
“High levels of succinate and fumarate can inhibit PHD2 activity through product-mediated inhibition of PHD function, leading to HIF-1α protein stabilization under normoxic (20% O2) conditions (100, 101).”
From Fueling Immunity: Insights into Metabolism and Lymphocyte Function (2013) · cites this paper as [101]
In the citing paper
“Succinate was initially found to impair PHD2, the α-ketoglutarate-dependent enzyme regulating HIF stability, through product inhibition (Selak et al., 2005).”
From Metabolic Reprogramming: A Cancer Hallmark Even Warburg Did Not Anticipate (2012) · cites this paper as Selak et al., 2005
In the citing paper
“elevated levels of succinate or fumarate can inhibit PHD activity [65, 66]”
From Hypoxia and hypoxia-inducible factors as regulators of T cell development, differentiation, and function (2013) · cites this paper as [66]
In the citing paper
“Both succinate and fumarate are competitive PHD inhibitors, and the massive accumulation of these metabolites in cells lacking SDH or FH activity elicit a hypoxic response even under oxygen-replete condition (Isaacs et al., 2005; Selak et al., 2005).”
From Cellular Metabolism and Disease: What Do Metabolic Outliers Teach Us? (2012) · cites this paper as Selak et al., 2005
In the citing paper
“Furthermore, both succinate and fumarate aberrantly increase the function of hypoxia inducible factors (HIFs).”
From Cellular Metabolism and Disease: What Do Metabolic Outliers Teach Us? (2012) · cites this paper as Selak et al., 2005
In the citing paper
“It should be noted that hypoxia and ischemia, as well as decreased oxidative phosphorylation, drive succinate accumulation in the mitochondrial matrix26,27.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [27]
In the citing paper
“Succinate inhibits the hydroxylation of HIF-1␣ by EGLN1, resulting in pseudohypoxic HIF-1␣ stabilization under normoxic conditions (10 -12).”
From Immunoresponsive Gene 1 and Itaconate Inhibit Succinate Dehydrogenase to Modulate Intracellular Succinate Levels (2016) · cites this paper as [10]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
Each contradiction between two passages listed here was confirmed by an independent check.
Di3 microscopy panel is described both with and without pVHL
MinorThe Figure 6A legend identifies panel v as the Di3 condition without pVHL. The results text instead describes the Di3 and Di4 comparisons in panels iv-vi as experiments cotransfected with HA-tagged pVHL, so the Di3 condition is inconsistent between the two descriptions.
“without pVHL (i, iii, v) or with pVHL (ii, iv, vi) together with one of the indicated siRNA constructs: scRNAi (i-iv), Di3 (v), or Di4 (vi).”
“When cells were cotransfected with GFP-ODD, HA-tagged pVHL, and Therefore, it is likely that in SDH-inhibited cells, the HIF-1␣ ODD domain is hydroxylated less efficiently, and consequently the either of the siRNA constructs, higher GFP levels were observed in Di3-and Di4-transfected cells compared to scRNAi-trans-binding of pVHL to GFP-ODD is reduced.”
Citations
Cites
Jaakkola, P., Mole, D. R., Tian, Y.-M., Wilson, M. I., Gielbert, J., Gaskell, S. J., Kriegsheim, A. V., Hebestreit, H. F., Mukherji, M., Schofield, C. J., et al. (2001) · Science · doi:10.1126/science.1059796 · Cited by 5,535 · cited 1×
“In particular, elevated levels al., 2001; Jaakkola et al., 2001; Yu et al., 2001).”
BackgroundHIFα Targeted for VHL-Mediated Destruction by Proline Hydroxylation: Implications for O 2 Sensing
Ivan, M., Kondo, K., Yang, H., Kim, W., Valiando, J., Ohh, M., Salic, A., Asara, J. M., Lane, W. S., & Kaelin Jr., W. G. (2001) · Science · doi:10.1126/science.1059817 · Cited by 4,735 · cited 1×
“, facilitate HIF-2 (cid:1) degradation in these cells, despite the fact that PHD activity is inhibited. and ascorbate, it undergoes hydroxylation and migrates faster CANCER CELL : JANUARY 2005 on SDS-PAGE (Huang et al., 2002; Ivan et al., 2001).”
BackgroundEpstein, A. C. R., Gleadle, J. M., McNeill, L. A., Hewitson, K. S., O'Rourke, J., Mole, D. R., Mukherji, M., Metzen, E., Wilson, M. I., Dhanda, A., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00507-4 · Cited by 3,333 · cited 1×
“The hydroxylases (HPH1-3 or PHD1-3) (Bruick and McKnight, 2001; Epstein et al., 2001; Ivan et al., 2002).”
A conserved family of prolyl-4-hydroxylases that modify HIF
Bruick, R. K., & McKnight, S. L. (2001) · Science · doi:10.1126/science.1066373 · Cited by 2,549 · cited 1×
“The hydroxylases (HPH1-3 or PHD1-3) (Bruick and McKnight, 2001; Epstein et al., 2001; Ivan et al., 2002).”
Regulation of angiogenesis by hypoxia: role of the HIF system
Pugh, C. W., & Ratcliffe, P. J. (2003) · Nature Medicine · doi:10.1038/nm0603-677 · Cited by 2,520 · cited 11×
“) and ascorbate as cofactors (Kaelin, 2002; Scho-field and Zhang, 1999).”
BackgroundMethodInfluentialA model for p53-induced apoptosis
Polyak, K., Xia, Y., Zweier, J. L., Kinzler, K. W., & Vogelstein, B. (1997) · Nature · doi:10.1038/38525 · Cited by 2,500 · cited 1×
“The tumor suppressor p53, which is known to elevate ROS production (Polyak et al., 1997), was used as a positive control.”
MethodOxygen sensing by HIF hydroxylases
Schofield, C. J., & Ratcliffe, P. J. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1366 · Cited by 2,068
Chandel, N. S., McClintock, D. S., Feliciano, C. E., Wood, T. M., Melendez, J. A., Rodriguez, A. M., & Schumacker, P. T. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m001914200 · Cited by 2,000 · cited 1×
“…c oxidase we found that hydrogen peroxide can induce HIF-1 (cid:1) levels as (complex IV), hence allocating more oxygen for PHD activity in was previously described (Chandel et al., 2000) (data not shown), we observed neither ROS generation nor a shift to a the cytosol (Hagen et al., 2003).”
BackgroundAsparagine Hydroxylation of the HIF Transactivation Domain: A Hypoxic Switch
Lando, D., Peet, D. J., Whelan, D. A., Gorman, J. J., & Whitelaw, M. L. (2002) · Science · doi:10.1126/science.1068592 · Cited by 1,558 · cited 1×
“The transcriptional activity of HIF-(cid:1) is also regulated by hydroxylation of an asparaginyl residue in its C-terminal transactivation domain in a reaction catalyzed by factor inhibiting HIF (FIH) (Hewitson et al., 2002; Lando et al., 2002a,2002b).”
BackgroundLando, D., Peet, D. J., Gorman, J. J., Whelan, D. A., Whitelaw, M. L., & Bruick, R. K. (2002) · Genes & Development · doi:10.1101/gad.991402 · Cited by 1,550
Flagged first, then by citations
2 of the 47 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- Beasley, 2002, Hypoxia-inducible factors HIF-1α and HIF-2α in head and neck cancer, Cancer Res., 62, 2493
Cited by
Sorted by citations
Items 1,601–1,700 of 2,106
Genetic insights into the hypoxia-inducible factor (HIF) pathway
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Biology of Head and Neck Cancer
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Carrera, S., Beristain, E., Sancho, A., Iruarrizaga, E., Rivero, P., Mañé, J. M., & López-Vivanco, G. (2019) · Hereditary Cancer in Clinical Practice · doi:10.1186/s13053-019-0124-6 · Cited by 6
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“For determination of SDH activity in PDECs, a colorimetric assay was used (adapted to [60]), which is based on the reduction of artificial electron acceptor 2,6-dichlorphenol-indophenol (DCIP).”
MethodBraun, M. M., Damjanac, T., Zhang, Y., Chen, C., Hu, J., & Maher, L. J., III (2019) · PLoS ONE · doi:10.1371/journal.pone.0227033 · Cited by 6
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“The mechanism of tumorigenesis in SDH-deficient cells has been shown to involve the accumulation of succinate in the cytoplasm, and its oncogenic effects are due to the inhibition of HIF-α prolyl hydroxylase.(34,35) Inactivation of SDHA allows for the accumulation of succinate.”
BackgroundSirtuiny – enzymy o wielokierunkowej aktywności katalitycznej
Kratz, E. M., Sołkiewicz, K., Kaczmarek, A., & Piwowar, A. (2021) · Postępy Higieny i Medycyny Doświadczalnej · doi:10.5604/01.3001.0014.7866 · Cited by 6
Genetic approaches to understand cellular responses to oxygen availability
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The Warburg Effect and Beyond: Metabolic Dependencies for Cancer Cells
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Paragangliomas in Carney–Stratakis Syndrome
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Krebs cycle enzymes as tumor suppressors
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Preclinical insights into the potential of itaconate and its derivatives for liver disease therapy
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“To test this idea, we investigated the therapeutic potential of dimethyl succinate (DMS), a membrane-permeable form of succinate [142], which can be metabolized to succinate to replenish the substrate required for the subsequent step in the TCA cycle, SDH.”
Nunes, P. R., Bruder, R. M., Kaihara, J. N. S., et al. (2025) · Placenta · doi:10.1016/j.placenta.2025.09.018 · Cited by 5
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“…O 2 ⋅ - ), activate succinate receptor 1 (SUCNR1), and upregulate hypoxia-inducible factor-1 α (HIF-1 α ) through inhibiting prolyl hydroxylase domain (PHD), thereby triggering the production of tumor necrosis factor-α (TNF-α ), interferon-γ (IFN-γ ), and interleukin-1 β (IL-1 β ) [82,83].”
Hypoxia in MASLD: a spatial determinant of the pathogenesis
Fuster‐Martínez, I., Monterde, V. B., Bidault, G., Arbonés-Mainar, J. M., & Vidal‐Puig, A. (2026) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2025.12.008 · Cited by 9
“Another mediator that promotes HIF-1 α stabilisation is succinate, a key intermediate in the tricarboxylic acid (TCA) cycle [51].”
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FAHD1 and mitochondrial metabolism: a decade of pioneering discoveries
Cappuccio, E., Holzknecht, M., Petit, M., Heberle, A., Rytchenko, Y., Seretis, A., Pierri, C. L., Gstach, H., Jansen‐Dürr, P., & Weiss, A. K. H. (2024) · FEBS Journal · doi:10.1111/febs.17345 · Cited by 7
“Several key intermediates, such as citrate, 2-oxoglutarate (2OG, a -ketoglutarate), succinyl-CoA, and oxaloacetate, can be diverted from the cycle to participate in the synthesis of lipids, amino acids, heme, and other essential molecules [53,54].”
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Molecular genetics of pheochromocytoma/paraganglioma
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“Additionally, fumarate reductase produces succinate that stabilises HIF-1, which promotes angiogenesis and facilitates cancer development (Selak et al .”
Brain Metabolic Adaptations to Hypoxia
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Energotropic Effects of Intermittent Hypoxia: Role of Succinate-Dependent Signaling
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Suffocation of gene expression
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Mir-roring hypoxia in EGFR-TKI tolerance
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Distinct metabolic alterations in different Caenorhabditis elegans mitochondrial mutants
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The Metabolism of Cell Growth and Proliferation
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Self-citationFumarate hydratase deficiency induces chronic myeloid leukemia progression
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“It has been identified that the stabilization of the hypoxia-inducible factor HIF-1α in HLRCC is because of the inhibition of PHDs (18).”
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“Known to be a prolyl hydroxylases inhibitor, succinic acid is able to stabilise hypoxia-inducible factors (HIFs) and activate genes involved in angiogenesis and energy metabolism [48].”
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Kinetic Analysis of HIF Prolyl Hydroxylases
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“Activation of mTORC1 by amino acids and growth signaling through RHEB induces glucose metabolism through increasing Myc and HIF-1 α activity and expression (Düvel et al. 2010).”
InfluentialExploring the oncogenic impact of heteroplasmic de novo MT-ND5 truncating mutations
Wu, Y., Ye, J., & Gu, Z. (2025) · Mitochondrial Communications · doi:10.1016/j.mitoco.2025.03.001 · Cited by 4
“These genetic alterations reprogram cellular metabolism and are implicated in the production of oncometabolites such as 2-hydroxyglutarate (2-HG), 13,18 succinate, 19 and fumarate.”
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“In these contexts, SDH deficiency and succinate accumulation promote tumorigenesis through stabilization of hypoxia-inducible factors and widespread epigenetic dysregulation (23).”
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PANoptosis in diabetes: immunometabolic insights and treatments
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“…- taining 4 (FNDC4)-a hepatokine/adipokine with anti-inflam - matory and metabolic regulatory functions—is significantly (HIF-1α) by inhibiting prolyl hydroxylase (PHD), thereby promoting glycolytic metabolism in DCs and inhibiting the activity of key tricarboxylic acid (TCA) cycle enzymes [167].”
Hypoxia-inducible factors link inflammation and lipid metabolism in atherosclerotic macrophages
Park, K. S., Ko, Y. J., & Choi, J.-H. (2026) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2026.1765661 · Cited by 5
Curtabbi, A., Jaroszewicz, S. N., Sanz-Cortés, R., Acín‐Pérez, R., Prymidis, D., Cherevatenko, M., de Mena, R. M., Esteban-Amo, M. J., de la Fuente, M. Á., Frezza, C., et al. (2026) · Nature Metabolism · doi:10.1038/s42255-026-01454-7 · Cited by 10
“This analysis revealed HIF1α as the most differentially active transcription factor, with a corresponding elevation in a group of its known target genes (Extended Data Fig.”
Alpha-ketoglutarate dehydrogenase: more than just a TCA cycle enzyme
Mailloux, R. J. (2026) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2026.02.050 · Cited by 5
Yang, K., Bi, J., Cao, Y., He, D., Fu, S., & Guo, W. (2026) · Journal of Advanced Research · doi:10.1016/j.jare.2026.03.048 · Cited by 4
The role of glycolysis in inflammation
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The interplay between STING and metabolic reprogramming: From mechanisms to therapeutic strategies
Zhou, L., Wang, Y., & Zhang, L. (2025) · International Immunopharmacology · doi:10.1016/j.intimp.2025.114987 · Cited by 4
Metabolites in the extracellular tumor microenvironment and the shaping of macrophage function
Lamorte, S., Bianca, M., Liu, Z. Q., & McGaha, T. L. (2025) · Molecular Cell · doi:10.1016/j.molcel.2025.09.016 · Cited by 4
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Cai, G., Hua, Z., Zhang, L., Chen, Y., Li, X., Ma, K., Xia, Z., & Li, Z. (2024) · Journal of Cellular Physiology · doi:10.1002/jcp.31175 · Cited by 4
“Macrohpage1 also displayed strong expression of HIF1A, which plays a crucial role in promoting cellular adaptation to low oxygen levels by inducing neovascularization and glycolysis (Celada et al., 2022; Eisenhofer et al., 2004; Selak et al., 2005) (Figure 3c).”
BackgroundIshii, T., Yasuda, K., Miyazawa, M., Onouchi, H., Yanase, S., & Ishii, N. (2025) · Journal of Clinical Biochemistry and Nutrition · doi:10.3164/jcbn.25-62 · Cited by 5
“(16,20,200) The chronic elevation in ROS levels generally results in damage to the various cellular components, which in turn results in the production of ROS at an even higher rate leading to oxida‐ tive stress.”
Duperret, L., Valdivieso, A., Kunselman, E., Petton, B., Morga, B., de Lorgeril, J., Pernet, F., Dégremont, L., Faury, N., Allienne, J., et al. (2025) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2025.180026 · Cited by 4
Modeling dioxygenase enzyme kinetics in familial paraganglioma
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“In SDH-deficient models, succinate accumulates and stabilizes HIF1α by inhibiting PHD2 (Letouze et al., 2013; MacKenzie et al., 2007; Selak et al., 2005; Xiao et al., 2012).”
BackgroundInfluentialAlsibai, K. D. (2014) · Journal of Cancer Prevention & Current Research · doi:10.15406/jcpcr.2014.01.00006 · Cited by 4
Pseudohypoxia, Mitochondrial Mutations, the Warburg Effect, and Cancer.
Sanders, E. (2012) · Cited by 4
Glycolytic Pathway as a Target for Tumor Inhibition
Lu, W., & Huang, P. (2010) · Springer eBooks · doi:10.1007/978-1-4419-6615-5_5 · Cited by 4
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Chinese Medicines for Cancer Treatment from the Metabolomics Perspective
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Generation of a hypoxia‐sensing mouse model
Lin, Q., Huang, Y., Giordano, F. J., & Yun, Z. (2019) · genesis · doi:10.1002/dvg.23352 · Cited by 3
“For the O2CreER fusion protein, we chose amino acids 371–653 of the HIF-1α ODD containing the canonical proline P402 and P564 whose hydroxylation leads to degradation of HIF-1α protein (Bishop & Ratcliffe, 2014; Huang et al., 1998; Semenza, 2007).”
InfluentialMitochondrial Signaling in Hypoxia
Lukyanova, L. D. (2013) · Open Journal of Endocrine and Metabolic Diseases · doi:10.4236/ojemd.2013.33029 · Cited by 4
The Genetic Basis of Kidney Cancer and Implications for Targeted Therapies
Schmidt, L. S., Srinivasan, R., & Linehan, W. M. (2012) · Springer eBooks · doi:10.1007/978-1-4614-2400-0_1 · Cited by 4
Winkelmann, I., Diehl, D., Oesterle, D., Daniel, H., & Wenzel, U. (2010) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201000086 · Cited by 4
Physiology and Pathology of Mitochondrial Dehydrogenases
Romani, A. (2018) · InTech eBooks · doi:10.5772/intechopen.76403 · Cited by 4
“Inhibition of HIF-1a hydroxylation works in conjunction with the accumulation of reactive oxygen species occurring through the succinate dehydrogenase complex to stabilize HIF-1a and promote the formation of a stable and active HIF complex that promotes the expression of tumor-inducing genes [19].”
BackgroundMitochondrial Genome Instability in Cancer
Bianchi, N. O. (2010) · Cytogenetic and Genome Research · doi:10.1159/000300567 · Cited by 5
“HIF1 and HIF-1 heterodimerization generates HIF compounds which induce the expression of nuclear genes controlling tumor progression [Selak et al., 2005].”
BackgroundLactic Acid Bacteria Mediated Phenolic Bioactive Modulation From Fruit Systems For Health Benefits
Ankolekar, C. (2013) · ScholarWorks@UMassAmherst (University of Massachusetts Amherst) · doi:10.7275/hbya-c596 · Cited by 4
Role of Succinate Dehydrogenase in Cellular Respiration
Viswalingam, V., & Kumar, D. (2025) · Springer eBooks · doi:10.1007/978-981-96-6831-1_3 · Cited by 3
Fanglei, C. H. A. I., Liu, C., Liu, Y., & Zou, W. (2025) · Frontiers in Neurology · doi:10.3389/fneur.2025.1678595 · Cited by 3
Fang, Z., Li, M., Mai, Y., Luo, W., Wang, E., Liu, X., Yang, C., Shen, Z., & Liu, Y. (2025) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5c19205 · Cited by 3
How do immunometabolites shape bacterial infections?
Gowdy, G., & Prince, A. S. (2026) · PLoS Biology · doi:10.1371/journal.pbio.3003585 · Cited by 4
“Succinate also inhibits HIF-1 α prolyl-hydroxylases (PHDs), resulting in the de-repression of HIF-1 α , which subsequently translocates to the nucleus and promotes the transcription of myriad genes including IL1B [37,62].”
Cubiella, T., Alba‐Linares, J. J., San‐Juan‐Guardado, J., Suárez‐Priede, Á., Gomez-Suarez, N., Tous, M. D. C., Bancos, I., Villabona, C. M., Piñol, T. S., Tena, I., et al. (2026) · Communications Biology · doi:10.1038/s42003-026-09543-9 · Cited by 3
“However, germline mutations in SDHB , SDHD , SDHA , or SDHC (collectively SDHx ) disrupt this process, leading to oncometabolite accumulation that drives epigenetic reprogramming 6,7 and activation of hypoxia-inducible factor (HIF) target genes 8 – 10 .”
Epigenetics and disease progression in neuroendocrine neoplasms
Lobato, E. C., Lafranchi, A., Freitas-Castro, F., Fagundes, G. F. C., Sadiković, B., Lines, K. E., & Almeida, M. Q. (2026) · Endocrine Reviews · doi:10.1210/endrev/bnag005 · Cited by 3
Metabolic Vulnerabilities as a Therapeutic Target in Breast Cancer
Guo, S., & Addison, C. (2026) · Current Oncology · doi:10.3390/curroncol33020129 · Cited by 4
“Increased cellular succinate promotes tumor cell growth and survival through inhibition of HIF1-α prolyl hydroxylases resulting in HIF1 α stabilization [186].”
The hypoxic tumor microenvironment: Functional and metabolic reprogramming of key immune populations
Ojaroodi, A. F., Shokravi, S., Eskandarzadeh, S., Ghahremanzadeh, A., Alizadeh, F., Amirikar, F., Mobayen, G., Mahrooghi, D., Lahouty, M., Shamsasenjan, K., et al. (2026) · Pathology - Research and Practice · doi:10.1016/j.prp.2026.156443 · Cited by 5
Proteostasis Deregulation by Metabolism Drives the Hallmarks of Cancer
Venkitaraman, A. R. (2026) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-051424-052148 · Cited by 4
The clinical landscape of HIF2α inhibitors in oncology
Saad, E., Machaalani, M., McDermott, D. F., & Choueiri, T. K. (2026) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-026-01145-y · Cited by 4
“Additionally, the phase Ib–II STELLAR-009 (NCT06191796) trial was initiated to evaluate casdatifan in combination with zanzalintinib, a novel, multi-targeted TKI with activity against VEGFR, MET and the TAM kinases (TYRO3, AXL, MER), as well as the triplet combination of cas-datifan, zanzalintinib and nivolumab in pat…”
InfluentialChinopoulos, C. (2026) · Redox Biology · doi:10.1016/j.redox.2026.104224 · Cited by 3
“Succinate accumulation competitively inhibits HIF-α prolyl hydroxylases and supports HIF-α stabilization, linking ETC backpressure/MRS to reduced prolyl hydroxylation-dependent restraint of HIF [59] (Fig.”
InfluentialFoglia, B., Nurcis, J., Signorini, M., & Cannito, S. (2026) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2026.1823145 · Cited by 3
“Accumulated succinate stabilizes HIF-1 α and promotes IL-1 β production (Tannahill et al., 2013; Selak et al., 2005), while citrate export from mitochondria fuels NADPH generation and histone acetylation required for inflammatory gene expression (Balsa et al., 2020; Billingham et al., 2022).”
Long, Y., Xu, J., Xu, Z., Su, Z., Li, W., & Li, J. (2026) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2026.119692 · Cited by 3
Report of a vagal paraganglioma at the cervicothoracic junction
Yun, J., Kapustin, D. A., Omorogbe, A., et al. (2023) · Head & Neck · doi:10.1002/hed.27481 · Cited by 3
“This leads to the activation of hypoxia-inducible transcription factors and the false signaling of hypoxia in the cell, which are thought to promote tumor growth.(29,31) SDH mutations are inherited in an autosomal dominant manner, and specifically D subunit mutations are inherited paternally.”
BackgroundBale, A. A., Thammineni, S., Bhargava, R., & Harley, B. A. C. (2024) · Advanced NanoBiomed Research · doi:10.1002/anbr.202400107 · Cited by 4
Xu, Q., Gan, R., Wang, Q., Cheng, P. C., Hu, Z., & Wang, J. (2025) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2025.117295 · Cited by 4
Mannose and PMI depletion overcomes radiation resistance in HPV-negative head and neck cancer
Wang, T., Brown, C., Doherty, N., Byrne, N. M., Islam, R., Doherty, M., Feng, J., Yin, C., Chambers, S. E. J., McQuoid, L., et al. (2025) · Cell Communication and Signaling · doi:10.1186/s12964-025-02204-0 · Cited by 3
Navigating Ongoing Challenges in GI Stromal Tumors
Joensuu, H., Miyashita, H., George, S., & Sicklick, J. K. (2025) · American Society of Clinical Oncology Educational Book · doi:10.1200/edbk-25-473224 · Cited by 3
PKM2: A novel helmsman of lipid metabolism
Liu, Z., Le, Y., & Lu, D. (2025) · Cellular Signalling · doi:10.1016/j.cellsig.2025.111967 · Cited by 3
Vanova, K. H., Uher, O., Kraus, M., Miklovicova, S., Hönigová, K., Gwiezdzinski, S., Garrett, T. J., Ghayee, H. K., Masařík, M., Wang, H., et al. (2025) · Cancer Letters · doi:10.1016/j.canlet.2025.217969 · Cited by 3
Singh, R., Parchwani, D. N., Sharma, G., & Sharma, P. (2025) · Indian Journal of Clinical Biochemistry · doi:10.1007/s12291-025-01341-0 · Cited by 3
“This inhibition blocks the activity of HIF hydroxylase, thereby enhancing HIF1-α activity [ 98 , 99 ].”
Neddylation: from regulatory mechanisms to clinical implications in cancer
Zhang, M., Peng, Q., Feng, Y., Yang, T., Yao, W., Deng, Z., & Wang, J. (2025) · Journal of Advanced Research · doi:10.1016/j.jare.2025.11.010 · Cited by 4
Mass Spectrometry-Based Profiling of Metabolites in Human Biofluids
Chakraborty, T., & Manna, S. K. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9027-6_12 · Cited by 3
Targeting the Redox Balance Pathway Using Ascorbic Acid in sdhb Zebrafish Mutant Larvae
Dona, M., Lamers, M., Rohde, S., Gorissen, M., & Timmers, H. J. L. M. (2021) · Cancers · doi:10.3390/cancers13205124 · Cited by 3
“On the other hand, two other studies reported no increased ROS levels in cell lines silenced for SDHB [30,31], despite hypoxia-inducible factor (HIF) stabilisation.”
BackgroundA Survey of the Metabolic Landscape of the Developing Cerebellum at Single-Cell Resolution
Krycer, J. R., & Nayler, S. (2022) · The Cerebellum · doi:10.1007/s12311-022-01415-2 · Cited by 3
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Metadata
- Authors
- Selak, M. A., Armour, S. M., MacKenzie, E. D., Boulahbel, H., Watson, D. G., Mansfield, K. D., Pan, Y., Simon, M. C., Thompson, C. B., & Gottlieb, E.
- Affiliations
- Cancer Research UK (GB)
- University of Pennsylvania (US)
- University of Strathclyde (GB)
- Corresponding author
- Gottlieb, Eyal
- ORCID
- Venue
- Cancer Cell
- Publisher
- Elsevier BV
- Published
- 2005-01
- Volume
- 7
- Issue
- 1
- Pages
- 77-85
- ISSN
- 1535-6108 (ISSN-L)
- OpenAlex
- W2057199368
- Cited by
- 2,108
- References
- 47
- Citations by year
- 2026: 110 · 2025: 115 · 2024: 97 · 2023: 97 · 2022: 116 · 2021: 139 · 2020: 128 · 2019: 123 · 2018: 93 · 2017: 107 · 2016: 110 · 2015: 107 · 2014: 108 · 2013: 109 · 2012: 86
- FWCI
- 26.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cancer Cell (publishedVersion)
- PubMed (publishedVersion)
- Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (acceptedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- Beatson Institute for Cancer Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Oncology › Ear and Head Tumors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Ferroptosis and cancer prognosis
- Keywords
- succinic acid, succinate dehydrogenase, HIF-1α, prolyl hydroxylase, mitochondrial dysfunction, tumor suppressor proteins, carcinogenesis, VHL, tumor angiogenesis, fumarate hydratase
- MeSH terms
- Animals, Cell Line, Cell Transformation, Neoplastic, Citric Acid Cycle, Enzyme Activation, Humans, Mitochondria, Oncogenes, Procollagen-Proline Dioxygenase, Succinate Dehydrogenase, Transcription Factors, Signal Transduction, Gene Expression Regulation, Enzymologic, Reactive Oxygen Species, Succinic Acid, Tumor Suppressor Proteins, RNA, Small Interfering, Ubiquitin-Protein Ligases, Von Hippel-Lindau Tumor Suppressor Protein, Hypoxia-Inducible Factor 1, alpha Subunit
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
A language model drew the claims from the paper on 2026-10-11, under version 3 of the claim definition (model deepseek-v4.1-flash, prompt extract-v3).
A second model labelled each claim on 2026-10-11 (model jev-1.13-20260917, definition revision gold-guideline-v0.2).
Cited sources are checked with a language model’s help (gpt-6-sol).
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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