Molecular Architecture and Functional Model of the Complete Yeast ESCRT-I Heterotetramer
Open accessAbstract
The endosomal sorting complex required for transport-I (ESCRT-I) complex, which is conserved from yeast to humans, directs the lysosomal degradation of ubiquitinated transmembrane proteins and the budding of the HIV virus. Yeast ESCRT-I contains four subunits, Vps23, Vps28, Vps37, and Mvb12. The crystal structure of the heterotetrameric ESCRT-I complex reveals a highly asymmetric complex of 1:1:1:1 subunit stoichiometry. The core complex is nearly 18 nm long and consists of a headpiece attached to a 13 nm stalk. The stalk is important for cargo sorting by ESCRT-I and is proposed to serve as a spacer regulating the correct disposition of cargo and other ESCRT components. Hydrodynamic constraints and crystallographic structures were used to generate a model of intact ESCRT-I in solution. The results show how ESCRT-I uses a combination of a rigid stalk and flexible tethers to interact with lipids, cargo, and other ESCRT complexes over a span of approximately 25 nm.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The ESCRT-I complex has a globular head with descending alpha helical hairpins, VPS28 top, VPS37 bottom, and a coiled-coil stalk.
“The complex assumes the structure of a globular head that is oriented as a descending stack of alpha helical hairpins with VPS28 on the top and VPS37 on the bottom, with a protruding coiled-coil stalk [29,30,31,32].”
From Budding of a Retrovirus: Some Assemblies Required (2020) · cites this paper as [31]
The functional human ESCRT-I complex is a hetero-tetramer of VPS28, TSG101, VPS37, and MVB12.
“the functional ESCRT-I complex in humans organizes itself as a hetero-tetramer composed of a single copy of VPS28, TSG101, VPS37, and MVB12.”
From Budding of a Retrovirus: Some Assemblies Required (2020) · cites this paper as [31]
Cites
Kirchhausen, T. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.699 · Cited by 560 · see citing works
Role of ESCRT-I in Retroviral Budding
Martin-Serrano, J., Zang, T., & Bieniasz, P. D. (2003) · Journal of Virology · doi:10.1128/jvi.77.8.4794-4804.2003 · Cited by 250 · see citing works
Endosomal and non-endosomal functions of ESCRT proteins
Slagsvold, T., Pattni, K., Malerød, L., & Stenmark, H. (2006) · Trends in Cell Biology · doi:10.1016/j.tcb.2006.04.004 · Cited by 238 · see citing works
Structural Basis for Endosomal Targeting by the Bro1 Domain
Kim, J., Sitaraman, S., Hierro, A., Beach, B. M., Odorizzi, G., & Hurley, J. H. (2005) · Developmental Cell · doi:10.1016/j.devcel.2005.04.001 · Cited by 174 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Addinall, S. G., Downey, M., Yu, M., Zubko, M. K., Dewar, J., Leake, A., Hallinan, J., Shaw, O., James, K., Wilkinson, D. J., et al. (2008) · Genetics · doi:10.1534/genetics.108.092577 · Cited by 83 · see citing works
Global Analysis of Yeast Endosomal Transport Identifies the Vps55/68 Sorting Complex
Schluter, C., Lam, K. K. Y., Brumm, J., Wu, B. W., Saunders, M., Stevens, T. H., Bryan, J., & Conibear, E. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-07-0659 · Cited by 36 · see citing works
Self-citationBudding of a Retrovirus: Some Assemblies Required
Rose, K. M., Hirsch, V. M., & Bouamr, F. (2020) · Viruses · doi:10.3390/v12101188 · Cited by 32 · see citing works
- Authors
- Kostelansky, M. S., Schluter, C., Tam, Y. Y. C., Lee, S., Ghirlando, R., Beach, B., Conibear, E., & Hurley, J. H.
- Affiliations
- National Institutes of Health (US)
- University of British Columbia (CA)
- Corresponding author
- Hurley, James H.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2007-05
- Volume
- 129
- Issue
- 3
- Pages
- 485-498
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC2065850
- OpenAlex
- W1976916223
- Cited by
- 172
- References
- 4
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 13 · 2023: 9 · 2022: 10 · 2021: 9 · 2020: 11 · 2019: 4 · 2018: 6 · 2017: 6 · 2016: 4 · 2015: 5 · 2014: 2 · 2013: 9 · 2012: 13
- FWCI
- 6.76
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (Z01DK036118)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Keywords
- ESCRT-I, heterotetramer, VPS28, yeast, crystal structure, stalk, cargo sorting, lysosomal degradation, HIV budding, solution structure, lipid interactions, protein complex structure
- MeSH terms
- Cell Membrane, Models, Molecular, Protein Conformation, Endosomes, Saccharomyces cerevisiae, Molecular Structure, Crystallography, X-Ray, Protein Structure, Quaternary, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, Multiprotein Complexes, Endosomal Sorting Complexes Required for Transport
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
