Global Analysis of Yeast Endosomal Transport Identifies the Vps55/68 Sorting Complex
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Endosomal transport is critical for cellular processes ranging from receptor down-regulation and retroviral budding to the immune response. A full understanding of endosome sorting requires a comprehensive picture of the multiprotein complexes that orchestrate vesicle formation and fusion. Here, we use unsupervised, large-scale phenotypic analysis and a novel computational approach for the global identification of endosomal transport factors. This technique effectively identifies components of known and novel protein assemblies. We report the characterization of a previously undescribed endosome sorting complex that contains two well-conserved proteins with four predicted membrane-spanning domains. Vps55p and Vps68p form a complex that acts with or downstream of ESCRT function to regulate endosomal trafficking. Loss of Vps68p disrupts recycling to the TGN as well as onward trafficking to the vacuole without preventing the formation of lumenal vesicles within the MVB. Our results suggest the Vps55/68 complex mediates a novel, conserved step in the endosomal maturation process.
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The predicted structure of the interaction between Vps55 and Vps68 provides clues about the mechanism of their function.
“Vps55 and Vps68 are conserved membrane proteins that are important for endosomal cargo sorting; our predicted structure (Fig. 2) of their interaction provides clues about the mechanism of their function (74).”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [74]
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R: A Language and Environment for Statistical Computing
R Core Team (2000) · doi:10.32614/r.manuals · Cited by 355,706 · see citing works
Cytoscape: A software Environment for integrated models of biomolecular interaction networks
Shannon, P., Markiel, A., Ozier, O., Baliga, N. S., Wang, J. T., Ramage, D., Amin, N., Schwikowski, B., & Ideker, T. (2003) · Genome Research · doi:10.1101/gr.1239303 · Cited by 51,888 · see citing works
Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248 · cited 5× · see citing works
“Genome-wide localization data (Huh et al., 2003) was simplified as follows to reduce the number of categories: vacuole vacuole/vacuolar membrane; nucleus nucleus/nucleolus; bud bud/budneck/cell periphery; endoplasmic reticulum (ER) ER/ nuclear periphery; and Golgi Golgi/Golgi-to-vacuole/ER-to-Golgi/Golgito-ER.”
BackgroundMethodInfluentialWinzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., et al. (1999) · Science · doi:10.1126/science.285.5429.901 · Cited by 3,953 · see citing works
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae
Krogan, N. J., Cagney, G., Yu, H., Zhong, G., Guo, X., Ignatchenko, A., Li, J., Pu, S., Datta, N., Tikuisis, A., et al. (2006) · Nature · doi:10.1038/nature04670 · Cited by 2,915 · see citing works
Proteome survey reveals modularity of the yeast cell machinery
Gavin, A., Aloy, P., Grandi, P., Krause, R., Böesche, M., Marzioch, M., Rau, C., Jensen, L. J., Bastuck, S., Dümpelfeld, B., et al. (2006) · Nature · doi:10.1038/nature04532 · Cited by 2,544 · see citing works
Guide to Yeast Genetics and Molecular Biology
Kurtzman, C. P., Guthrie, C., & Fink, G. R. (1993) · Mycologia · doi:10.2307/3760517 · Cited by 2,382 · see citing works
Global Mapping of the Yeast Genetic Interaction Network
Tong, A. H. Y., Lesage, G., Bader, G. D., Ding, H., Xu, H., Xin, X., Young, J., Berriz, G. F., Brost, R. L., Chang, M., et al. (2004) · Science · doi:10.1126/science.1091317 · Cited by 2,070 · see citing works
ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex
Mizuguchi, G., Shen, X., Landry, J., Wu, W.-H., Sen, S., & Wu, C. (2003) · Science · doi:10.1126/science.1090701 · Cited by 1,312 · see citing works
Receptor downregulation and multivesicular-body sorting
Katzmann, D. J., Odorizzi, G., & Emr, S. D. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm973 · Cited by 1,201 · see citing works
Guide to yeast genetics and molecular biology
Simanis, V. (1992) · Trends in Cell Biology · doi:10.1016/0962-8924(92)90020-n · Cited by 1,003 · see citing works
Schuldiner, M., Collins, S. R., Thompson, N. J., Denic, V., Bhamidipati, A., Punna, T., Ihmels, J., Andrews, B., Boone, C., Greenblatt, J. F., et al. (2005) · Cell · doi:10.1016/j.cell.2005.08.031 · Cited by 870 · see citing works
Collins, S. R., Miller, K. M., Maas, N. L., Roguev, A., Fillingham, J., Chu, C. S., Schuldiner, M., Gebbia, M., Recht, J., Shales, M., et al. (2007) · Nature · doi:10.1038/nature05649 · Cited by 869 · see citing works
The Protein Network of HIV Budding
von Schwedler, U. K., Stuchell‐Brereton, M. D., Müller, B., Ward, D. M., Chung, H.-Y., Morita, E., Wang, H. E., Davis, T., He, G.-P., Cimbora, D. M., et al. (2003) · Cell · doi:10.1016/s0092-8674(03)00714-1 · Cited by 815 · see citing works
The biogenesis of multivesicular endosomes
Gruenberg, J. E., & Stenmark, H. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1360 · Cited by 723 · see citing works
Retrograde transport from endosomes to the trans-Golgi network
Bonifacino, J. S., & Rojas, R. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1985 · Cited by 668 · see citing works
Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
Seaman, M. N. J. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200312034 · Cited by 624 · see citing works
Kobor, M. S., Venkatasubrahmanyam, S., Meneghini, M. D., Gin, J., Jennings, J. L., Link, A. J., Madhani, H. D., & Rine, J. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020131 · Cited by 607 · see citing works
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
Arighi, C. N., Hartnell, L. M., Aguilar, R. C., Haft, C. R., & Bonifacino, J. S. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200312055 · Cited by 601 · see citing works
A Snf2 Family ATPase Complex Required for Recruitment of the Histone H2A Variant Htz1
Krogan, N. J., Keogh, M., Datta, N., Sawa, C., Ryan, O. W., Ding, H., Haw, R., Pootoolal, J., Tong, A. H. Y., Canadien, V., et al. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00497-0 · Cited by 588 · see citing works
Morita, E., & Sundquist, W. I. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.102350 · Cited by 574 · see citing works
Stevens, T. H., Esmon, B., & Schekman, R. W. (1982) · Cell · doi:10.1016/0092-8674(82)90241-0 · Cited by 572 · see citing works
Self-citationTHE ESCRT COMPLEXES: Structure and Mechanism of a Membrane-Trafficking Network
Hurley, J. H., & Emr, S. D. (2006) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.35.040405.102126 · Cited by 546 · see citing works
Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast
Parsons, A. B., López, A., Givoni, I. E., Williams, D. E., Gray, C. A., Porter, J., Chua, G., Sopko, R., Brost, R. L., Ho, C.-H., et al. (2006) · Cell · doi:10.1016/j.cell.2006.06.040 · Cited by 500 · see citing works
Rothman, J. H., & Stevens, T. H. (1986) · Cell · doi:10.1016/0092-8674(86)90819-6 · Cited by 428 · see citing works
Self-citationPiper, R. C., Cooper, A. A., Yang, H., & Stevens, T. H. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.131.3.603 · Cited by 382 · see citing works
Self-citationSpecific Retrieval of the Exocytic SNARE Snc1p from Early Yeast Endosomes
Lewis, M. J., Nichols, B. J., Prescianotto‐Baschong, C., Riezman, H., & Pelham, H. R. B. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.1.23 · Cited by 361 · see citing works
Darsow, T., Rieder, S., & Emr, S. D. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.3.517 · Cited by 358 · see citing works
Sorting of proteins into multivesicular bodies: ubiquitin‐dependent and ‐independent targeting
Reggiori, F., & Pelham, H. R. B. (2001) · The EMBO Journal · doi:10.1093/emboj/20.18.5176 · Cited by 324 · see citing works
Genomic Screen for Vacuolar Protein Sorting Genes inSaccharomyces cerevisiae
Bonangelino, C. J., Chavez, E. M., & Bonifacino, J. S. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.02-01-0005 · Cited by 314 · see citing works
Protein transport from the late Golgi to the vacuole in the yeast Saccharomyces cerevisiae
Bowers, K., & Stevens, T. H. (2005) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2005.04.004 · Cited by 310 · see citing works
Self-citationRecycle your receptors with retromer
Seaman, M. N. J. (2005) · Trends in Cell Biology · doi:10.1016/j.tcb.2004.12.004 · Cited by 309 · see citing works
Systematic pathway analysis using high-resolution fitness profiling of combinatorial gene deletions
St.Onge, R. P., Mani, R., Oh, J., Proctor, M. J., Fung, E., Davis, R. W., Nislow, C., Roth, F. P., & Giaever, G. N. (2007) · Nature Genetics · doi:10.1038/ng1948 · Cited by 306 · see citing works
Systematic genome-wide screens of gene function
Carpenter, A. E., & Sabatini, D. M. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1248 · Cited by 296 · see citing works
Cooper, A. A., & Stevens, T. H. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.133.3.529 · Cited by 291 · see citing works
Self-citationConibear, E., & Stevens, T. H. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.1.305 · Cited by 290 · see citing works
Self-citationBehnia, R., Panic, B., Whyte, J. R. C., & Munro, S. (2004) · Nature Cell Biology · doi:10.1038/ncb1120 · Cited by 252 · see citing works
Activation of the Phosphatidylinositol 3-Kinase Vps34 by a G Protein α Subunit at the Endosome
Slessareva, J. E., Routt, S. M., Temple, B. R. S., Bankaitis, V. A., & Dohlman, H. G. (2006) · Cell · doi:10.1016/j.cell.2006.04.045 · Cited by 233 · see citing works
Cowles, C. R., Snyder, W. B., Burd, C. G., & Emr, S. D. (1997) · The EMBO Journal · doi:10.1093/emboj/16.10.2769 · Cited by 219 · see citing works
Burda, P., Padilla, S. M., Sarkar, S., & Emr, S. D. (2002) · Journal of Cell Science · doi:10.1242/jcs.00090 · Cited by 216 · see citing works
Nothwehr, S. F., Ha, S.-A., & Bruinsma, P. J. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.2.297 · Cited by 196 · see citing works
Setty, S. R. G., Strochlic, T. I., Tong, A. H. Y., Boone, C. M., & Burd, C. G. (2004) · Nature Cell Biology · doi:10.1038/ncb1121 · Cited by 174 · see citing works
Bowers, K., Levi, B. P., Patel, F. I., & Stevens, T. H. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.12.4277 · Cited by 173 · see citing works
Self-citationMolecular Architecture and Functional Model of the Complete Yeast ESCRT-I Heterotetramer
Kostelansky, M. S., Schluter, C., Tam, Y. Y. C., Lee, S., Ghirlando, R., Beach, B., Conibear, E., & Hurley, J. H. (2007) · Cell · doi:10.1016/j.cell.2007.03.016 · Cited by 172 · see citing works
Self-citationCereghino, J. L., Marcusson, E. G., & Emr, S. D. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.9.1089 · Cited by 158 · see citing works
Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1
Azmi, I. F., Davies, B. A., Dimaano, C., Payne, J. A., Eckert, D. M., Babst, M., & Katzmann, D. J. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200508166 · Cited by 155 · see citing works
Piper, R. C., Bryant, N. J., & Stevens, T. H. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.3.531 · Cited by 154 · see citing works
Self-citationUbiquitin Sorts Proteins into the Intralumenal Degradative Compartment of the Late‐Endosome/Vacuole
Urbanowski, J. L., & Piper, R. C. (2001) · Traffic · doi:10.1034/j.1600-0854.2001.20905.x · Cited by 151 · see citing works
A Probability Theory of Cluster Analysis
Ling, R. F. (1973) · Journal of the American Statistical Association · doi:10.1080/01621459.1973.10481356 · Cited by 134 · see citing works
Yang, X., Matern, H. T., & Gallwitz, D. (1998) · The EMBO Journal · doi:10.1093/emboj/17.17.4954 · Cited by 128 · see citing works
Palmitoylation by the DHHC protein Pfa4 regulates the ER exit of Chs3
Lam, K., Davey, M., Sun, B., Roth, A. F., Davis, N. G., & Conibear, E. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200602049 · Cited by 117 · see citing works
Self-citationStructure and Assembly of the Yeast V-ATPase
Graham, L. A., Flannery, A. R., & Stevens, T. H. (2003) · Journal of Bioenergetics and Biomembranes · doi:10.1023/a:1025772730586 · Cited by 116 · see citing works
Self-citationBryant, N. J., & Stevens, T. H. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.136.2.287 · Cited by 100 · see citing works
Self-citationProteinase C (carboxypeptidase Y) mutant of yeast
Wolf, D., & Fink, G. (1975) · Journal of Bacteriology · doi:10.1128/jb.123.3.1150-1156.1975 · Cited by 89 · see citing works
Lottridge, J. M., Flannery, A. R., Vincelli, J. L., & Stevens, T. H. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601712103 · Cited by 89 · see citing works
Self-citationCurtiss, M., Jones, C. B., & Babst, M. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-07-0588 · Cited by 73 · see citing works
Stawiecka-Mirota, M., Pokrzywa, W., Morvan, J., Żołądek, T., Haguenauer‐Tsapis, R., Urban‐Grimal, D., & Morsomme, P. (2007) · Traffic · doi:10.1111/j.1600-0854.2007.00610.x · Cited by 71 · see citing works
The leptin receptor promoter controls expression of a second distinct protein
Bailleul, B. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.14.2752 · Cited by 68 · see citing works
Bryant, N. J., Piper, R. C., Gerrard, S. R., & Stevens, T. H. (1998) · European Journal of Cell Biology · doi:10.1016/s0171-9335(98)80016-2 · Cited by 66 · see citing works
Self-citationNew component of ESCRT-I regulates endosomal sorting complex assembly
Chu, T., Sun, J., Saksena, S., & Emr, S. D. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200608053 · Cited by 65 · see citing works
Recycling of the Yeast a-Factor Receptor
Chen, L., & Davis, N. G. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.3.731 · Cited by 63 · see citing works
Ubiquitin‐Independent Entry into the Yeast Recycling Pathway
Chen, L., & Davis, N. G. (2002) · Traffic · doi:10.1034/j.1600-0854.2002.030204.x · Cited by 57 · see citing works
Conibear, E., & Stevens, T. H. (2002) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(02)51861-9 · Cited by 54 · see citing works
Self-citationMvb12 Is a Novel Member of ESCRT-I Involved in Cargo Selection by the Multivesicular Body Pathway
Oestreich, A. J., Davies, B. A., Payne, J. A., & Katzmann, D. J. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-07-0601 · Cited by 53 · see citing works
Belgareh‐Touzé, N., Avaro, S., Rouillé, Y., Hoflack, B., & Haguenauer‐Tsapis, R. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.01-12-0597 · Cited by 44 · see citing works
Conibear, E., & Tam, Y. (2009) · Springer eBooks · doi:10.1007/978-0-387-93877-6_4 · Cited by 35 · see citing works
Self-citationNBD-labeled phosphatidylcholine enters the yeast vacuole via the pre-vacuolar compartment
Hanson, P. K., Grant, A. M., & Nichols, J. L. (2002) · Journal of Cell Science · doi:10.1242/jcs.115.13.2725 · Cited by 29 · see citing works
(2006) · Springer eBooks · doi:10.1007/3-540-29719-0_969 · Cited by 20 · see citing works
Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
Burston, H. E., Davey, M., & Conibear, E. (2008) · Methods in molecular biology · doi:10.1007/978-1-59745-261-8_3 · Cited by 9 · see citing works
Self-citationToward the Systems Biology of Vesicle Transport
Quenneville, N. R., & Conibear, E. (2006) · Traffic · doi:10.1111/j.1600-0854.2006.00436.x · Cited by 7 · see citing works
Self-citationConibear, E. (2005) · Cell · doi:10.1016/j.cell.2005.10.016 · Cited by 5 · see citing works
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Computed structures of core eukaryotic protein complexes
Humphreys, I. R., Pei, J., Baek, M., Krishnakumar, A., Anishchenko, I., Ovchinnikov, S., Zhang, J., Ness, T. J., Banjade, S., Bagde, S. R., et al. (2021) · Science · doi:10.1126/science.abm4805 · Cited by 542 · see citing works
The Yeast Deletion Collection: A Decade of Functional Genomics
Giaever, G. N., & Nislow, C. (2014) · Genetics · doi:10.1534/genetics.114.161620 · Cited by 478 · see citing works
Delivery of endocytosed membrane proteins to the lysosome
Pryor, P. R., & Luzio, J. P. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2008.12.022 · Cited by 129 · see citing works
An endocytic mechanism for haemoglobin‐iron acquisition in <i>Candida albicans</i>
Weissman, Z., Shemer, R., Conibear, E., & Kornitzer, D. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06277.x · Cited by 127 · see citing works
Self-citationRegulators of yeast endocytosis identified by systematic quantitative analysis
Burston, H. E., Maldonado‐Báez, L., Davey, M., Montpetit, B., Schluter, C., Wendland, B., & Conibear, E. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200811116 · Cited by 120 · see citing works
Self-citationOn the Molecular Evolution of Leptin, Leptin Receptor, and Endospanin
Londraville, R. L., Prokop, J. W., Duff, R. J., Liu, Q., & Tuttle, M. A. (2017) · Frontiers in Endocrinology · doi:10.3389/fendo.2017.00058 · Cited by 70 · see citing works
Genome‐wide identification of viral and host transcripts targeted by viral siRNAs in V itis vinifera
Miozzi, L., Gambino, G., Burgyán, J., & Pantaleo, V. (2012) · Molecular Plant Pathology · doi:10.1111/j.1364-3703.2012.00828.x · Cited by 68 · see citing works
LEPROT and LEPROTL1 cooperatively decrease hepatic growth hormone action in mice
Touvier, T., Conte-Auriol, F., Briand, O., Cudejko, C., Paumelle, R., Caron, S., Baugé, E., Rouillé, Y., Salles, J., Staels, B., et al. (2009) · Journal of Clinical Investigation · doi:10.1172/jci34997 · Cited by 59 · see citing works
Multiple Signals Converge on a Differentiation MAPK Pathway
Chavel, C. A., Dionne, H. M., Birkaya, B. G., Joshi, J., & Cullen, P. J. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1000883 · Cited by 54 · see citing works
Endospanins Regulate a Postinternalization Step of the Leptin Receptor Endocytic Pathway
Séron, K., Couturier, C., Belouzard, S., Bacart, J., Monté, D., Corset, L., Bocquet, O., Dam, J., Vauthier, V., Lecœur, C., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.224857 · Cited by 48 · see citing works
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Gelling, C. L., Dawes, I. W., Perlmutter, D. H., Fisher, E. A. A., & Brodsky, J. L. (2012) · Genetics · doi:10.1534/genetics.112.143487 · Cited by 48 · see citing works
Identification of the Novel TRAPP Associated Protein Tca17
Montpetit, B., & Conibear, E. (2009) · Traffic · doi:10.1111/j.1600-0854.2009.00895.x · Cited by 48 · see citing works
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Park, Y., Chen, S. H., Camacho, E., Casadevall, A., & Williamson, P. R. (2020) · Infection and Immunity · doi:10.1128/iai.00954-19 · Cited by 41 · see citing works
Essary, B. D., & Marshall, P. A. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.05.018 · Cited by 41 · see citing works
Hung, C., Martínez-Márquez, J. Y., Javed, F. T., & Duncan, M. C. (2018) · Scientific Reports · doi:10.1038/s41598-018-30305-z · Cited by 40 · see citing works
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Ricarte, F. R., Menjivar, R. E., Chhun, S., Soreta, T., Oliveira, L., Hsueh, T., Serranilla, M., & Gharakhanian, E. (2011) · PLoS ONE · doi:10.1371/journal.pone.0023696 · Cited by 27 · see citing works
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Troutman, K. K., Varlakhanova, N. V., Tornabene, B. A., Ramachandran, R., & Ford, M. G. J. (2022) · Journal of Cell Science · doi:10.1242/jcs.259994 · Cited by 10 · see citing works
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Styles, E. B., Youn, J., Ušaj, M. M., & Andrews, B. (2013) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2013.0118 · Cited by 9 · see citing works
Matsumoto, R., Suzuki, K., & Ohya, Y. (2013) · PROTOPLASMA · doi:10.1007/s00709-013-0510-2 · Cited by 6 · see citing works
Yang, Y., Liu, Q., Jiang, G., Chen, S., Zhou, L., Sakamoto, N., Kuno, T., Fang, Y., & Yao, F. (2018) · PLoS ONE · doi:10.1371/journal.pone.0198516 · Cited by 5 · see citing works
Discovery and Expansion of Gene Modules by Seeking Isolated Groups in a Random Graph Process
Brumm, J., Conibear, E., Wasserman, W. W., & Bryan, J. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003358 · Cited by 4 · see citing works
Self-citationComponents of an ESCRT-independent nuclear envelope assembly pathway
Sydir, E. M., Farra, M. H., Whitford, A. L., Hinojosa, S., Kao, P.-Y., Paulo, J. A., Swarup, S., Lusk, C. P., Harper, J. W., Lee, I., et al. (2026) · The Journal of Cell Biology · doi:10.1083/jcb.202601189 · Cited by 2 · see citing works
Interaction between ESCRT-III proteins and the yeast SERINC homolog Tms1
Kölling, R. (2024) · Genetics · doi:10.1093/genetics/iyae132 · Cited by 1 · see citing works
Small effect-size mutations cumulatively affect yeast quantitative traitsPreprint
Hua, B., & Springer, M. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/126409 · Cited by 1 · see citing works
Identification of gene modules using a generative model for relational data
Lacerda, G. (2010) · cIRcle (University of British Columbia) · doi:10.14288/1.0051953
(2009) · Yeast · doi:10.1002/yea.1619
Link between lipid remodeling and ESCRT-III function in multivesicular body formationPreprint
Kölling, R. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.10.561655
WITHDRAWN: The sorting nexin requirement of the Vps68 recycling signal is context dependentPreprint
Kölling, R. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.31.565015
Wang, J., Liu, M., Chen, Y., Chen, J., Zhong, X., Wang, Z., Jiang, X., & Tian, R. (2026) · Cell Reports · doi:10.1016/j.celrep.2026.117756
Jin, L., Xiao, J., Guo, Z., Chen, M., Jin, S., Niu, J., Zhou, Y., Ding, S., & Guo, Z. (2026) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiag631
Large eukaryotic DNA viruses encode ESCRT machinery for membrane remodelling
Medvedeva, S., Guyet, U., Koonin, E. V., Delmont, T. O., & Krupovìč, M. (2026) · Nature Microbiology · doi:10.1038/s41564-026-02470-9
- Authors
- Schluter, C., Lam, K. K. Y., Brumm, J., Wu, B. W., Saunders, M., Stevens, T. H., Bryan, J., & Conibear, E.
- Affiliations
- University of British Columbia (CA)
- University of Oregon (US)
- Venue
- Molecular Biology of the Cell
- Published
- 2008-04
- Volume
- 19
- Issue
- 4
- Pages
- 1282-1294
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC2291407
- OpenAlex
- W2109180315
- Cited by
- 36
- References
- 71
- Citations by year
- 2026: 4 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2018: 4 · 2017: 2 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.75
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wayne State University
- Michael Smith Health Research BC
- National Institutes of Health (GM32448)
- Canadian Institutes of Health Research
- Medical Research Council
- National Institute of General Medical Sciences (R01GM032448, GM-32448, R37GM032448)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- endosomal transport, ESCRT, multivesicular bodies, endosomal maturation, membrane-spanning proteins, protein complex identification, yeast
- MeSH terms
- Biological Transport, Active, Carboxypeptidases, Carrier Proteins, Genes, Fungal, Multigene Family, Membrane Proteins, Mutation, Phenotype, Endosomes, Saccharomyces cerevisiae, Gene Deletion, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, Multiprotein Complexes, Receptors, Mating Factor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
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