The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.
Sgs1 Helicase and Two Nucleases Dna2 and Exo1 Resect DNA Double-Strand Break Ends
Open accessAbstract
Summary Formation of single-strand DNA (ssDNA) tails at a double-strand break (DSB) is a key step in homologous recombination and DNA damage signaling. The enzyme(s) producing ssDNA at DSBs in eukaryotes remains unknown. We monitored 5’-strand resection at inducible DSB ends and identified proteins required for two stages of resection: initiation and long-range 5’-strand resection. The Mre11-Rad50-Xrs2 complex (MRX) initiates 5’ degradation, whereas Sgs1 and Dna2 degrade 5’-strands exposing long 3’-strands at 4.4 kb/h rate. Deletion of SGS1 or DNA2 reduces resection and DSB repair by single strand annealing between distant repeats. Resection in the absence of SGS1 or DNA2 depends on Exo1. In exo1Δ sgs1Δ mutants the MRX complex and Sae2 in a stepwise manner generate only few hundred nucleotides of ssDNA at the break resulting in inefficient gene conversion and G2/M damage checkpoint arrest. We provide the first comprehensive model of the early steps of DSB repair in eukaryotes.
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Cites
Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes
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Mechanism of Eukaryotic Homologous Recombination
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Human CtIP promotes DNA end resection
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The Bloom's syndrome helicase suppresses crossing over during homologous recombination
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ATM- and cell cycle-dependent regulation of ATR in response to DNA double-strand breaks
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Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair
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The 3′ to 5′ Exonuclease Activity of Mre11 Facilitates Repair of DNA Double-Strand Breaks
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Choreography of the DNA Damage Response
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Self-citationDNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1
Ira, G., Pellicioli, A., Balijja, A., Wang, X., Fiorani, S., Carotenuto, W., Liberi, G., Bressan, D., Wan, L., Hollingsworth, N. M., et al. (2004) · Nature · doi:10.1038/nature02964 · Cited by 722
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Cited by
Non-homologous DNA end joining and alternative pathways to double-strand break repair
Chang, H. H. Y., Pannunzio, N. R., Adachi, N., & Lieber, M. R. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.48 · Cited by 1,757
Regulation of Homologous Recombination in Eukaryotes
Heyer, W.-D., Ehmsen, K. T., & Liu, J. (2010) · Annual Review of Genetics · doi:10.1146/annurev-genet-051710-150955 · Cited by 1,066
Targeting DNA damage response pathways in cancer
Groelly, F. J., Fawkes, M., Dagg, R. A., Blackford, A. N., & Tarsounas, M. (2023) · Nature Reviews Cancer · doi:10.1038/s41568-022-00535-5 · Cited by 836
The MRE11 complex: starting from the ends
Stracker, T. H., & Petrini, J. H. J. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3047 · Cited by 734
Protein Group Modification and Synergy in the SUMO Pathway as Exemplified in DNA Repair
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Metadata
- Authors
- Zhu, Z., Chung, W.-H., Shim, E. Y., Lee, S. E., & Ira, G.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2008-09
- Volume
- 134
- Issue
- 6
- Pages
- 981-994
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2094020226
- Cited by
- 1,051
- References
- 70
- FWCI
- 30.03
- Type
- journal article
- Language
- en
- Open access
- bronze
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