The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Mistranslation of Membrane Proteins and Two-Component System Activation Trigger Antibiotic-Mediated Cell Death
Open accessAbstract
Summary Aminoglycoside antibiotics directly target the ribosome, yet the mechanisms by which they induce cell death are not fully understood. Recently, oxidative stress has been implicated as one of the mechanisms whereby bactericidal antibiotics kill bacteria. Here we work out, using systems-level approaches and phenotypic analyses, the pathway whereby aminoglycosides ultimately trigger hydroxyl radical formation. We show, by disabling systems which facilitate membrane protein traffic, that mistranslation and misfolding of membrane proteins are central to aminoglycoside-induced oxidative stress and cell death. We also demonstrate that signaling through the envelope stress response two-component system is a key player in this process, and highlight an associated role for the redox-responsive two-component system. Additionally, we show that these two-component systems play a general role in bactericidal antibiotic-mediated oxidative stress and cell death, expanding our understanding of the common mechanism of killing induced by bactericidal antibiotics.
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Gene Ontology: tool for the unification of biology
Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P., Dolinski, K., Dwight, S. S., Eppig, J. T., et al. (2000) · Nature Genetics · doi:10.1038/75556 · Cited by 43,206
Statistical significance for genomewide studies
Storey, J. D., & Tibshirani, R. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1530509100 · Cited by 9,692
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
Boyle, E. I., Weng, S., Gollub, J., Jin, H., Botstein, D., Cherry, J. M., & Sherlock, G. (2004) · Bioinformatics · doi:10.1093/bioinformatics/bth456 · Cited by 2,067
Inferring Genetic Networks and Identifying Compound Mode of Action via Expression Profiling
Gardner, T. S., di Bernardo, D., Lorenz, D., & Collins, J. J. (2003) · Science · doi:10.1126/science.1081900 · Cited by 1,117
Self-citationHartl, F.-U., Lecker, S., Schiebel, E., Hendrick, J. P., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90160-g · Cited by 562
Protein translocation across biological membranes
Wickner, W., & Schekman, R. (2005) · Science · doi:10.1126/science.1113752 · Cited by 458
Salgado, H. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkj156 · Cited by 368
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Metabolite-enabled eradication of bacterial persisters by aminoglycosides
Allison, K. R., Brynildsen, M. P., & Collins, J. J. (2011) · Nature · doi:10.1038/nature10069 · Cited by 1,025
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Iorio, F., Bosotti, R., Scacheri, E., Belcastro, V., Mithbaokar, P., Ferriero, R., Murino, L., Tagliaferri, R., Brunetti-Pierri, N., Isacchi, A., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1000138107 · Cited by 816
Antibiotic efficacy is linked to bacterial cellular respiration
Lobritz, M. A., Belenky, P., Porter, C. B. M., Gutierrez, A., Yang, J. H., Schwarz, E. G., Dwyer, D. J., Khalil, A. S., & Collins, J. J. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1509743112 · Cited by 773
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Metadata
- Authors
- Kohanski, M. A., Dwyer, D. J., Wierzbowski, J., Cottarel, G., & Collins, J. J.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 135
- Issue
- 4
- Pages
- 679-690
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2035145746
- Cited by
- 574
- References
- 8
- FWCI
- 16.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kohanski, M. A., Dwyer, D. J., Wierzbowski, J., Cottarel, G., & Collins, J. J. (2008). Mistranslation of Membrane Proteins and Two-Component System Activation Trigger Antibiotic-Mediated Cell Death. Cell. https://doi.org/10.1016/j.cell.2008.09.038
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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