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Molecular Architecture of the Mos1 Paired-End Complex: The Structural Basis of DNA Transposition in a Eukaryote
Open accessAbstract
A key step in cut-and-paste DNA transposition is the pairing of transposon ends before the element is excised and inserted at a new site in its host genome. Crystallographic analyses of the paired-end complex (PEC) formed from precleaved transposon ends and the transposase of the eukaryotic element Mos1 reveals two parallel ends bound to a dimeric enzyme. The complex has a trans arrangement, with each transposon end recognized by the DNA binding region of one transposase monomer and by the active site of the other monomer. Two additional DNA duplexes in the crystal indicate likely binding sites for flanking DNA. Biochemical data provide support for a model of the target capture complex and identify Arg186 to be critical for target binding. Mixing experiments indicate that a transposase dimer initiates first-strand cleavage and suggest a pathway for PEC formation.
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“Intasomes from several retroviral species as well as prokaryotic and eukaryotic transpososomes have been visualised as post-catalytic strand transfer complexes, all of which display sharp deformations of target DNA29,30,32,33,37,51–54.”
From Structure and function of retroviral integrase (2022) · cites this paper as [51]
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Citations
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Items 101–146 of 146
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“The four TIR-containing oligonucleotides were designed based on prior knowledge of the Hsmar1 binding site within the TIR (Cordaux et al., 2006) and the available crystal structures of Tc3 and Mos1 complexed with DNA (Watkins et al., 2004; Richardson et al., 2009).”
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Metadata
- Authors
- Richardson, J. M., Colloms, S. D., Finnegan, D. J., & Walkinshaw, M. D.
- Affiliations
- University of Edinburgh (GB)
- Corresponding author
- Richardson, Julia M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2009-09
- Volume
- 138
- Issue
- 6
- Pages
- 1096-1108
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC3977044
- OpenAlex
- W2026862785
- Cited by
- 145
- References
- 39
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 7 · 2020: 6 · 2019: 6 · 2018: 7 · 2017: 8 · 2016: 10 · 2015: 17 · 2014: 14 · 2013: 12 · 2012: 11
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- Wellcome Trust (085176)
- Chinese Academy of Agricultural Sciences
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- DNA transposition, cut-and-paste transposition, crystal structure
- MeSH terms
- Animals, DNA Transposable Elements, DNA-Binding Proteins, Drosophila, Models, Molecular, X-Ray Diffraction, Protein Structure, Tertiary, Crystallography, X-Ray, Transposases, Catalytic Domain
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