The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Lipoprotein Cofactors Located in the Outer Membrane Activate Bacterial Cell Wall Polymerases
Open accessAbstract
Summary Most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by polysaccharide polymerases called penicillin-binding proteins (PBPs). Because they are the targets of penicillin and related antibiotics, the structure and biochemical functions of the PBPs have been extensively studied. Despite this, we still know surprisingly little about how these enzymes build the PG layer in vivo. Here, we identify the Escherichia coli outer membrane lipoproteins LpoA and LpoB as essential PBP cofactors. We show that LpoA and LpoB form specific trans-envelope complexes with their cognate PBP and are critical for PBP function in vivo. We further show that LpoB promotes PG synthesis by its partner PBP in vitro and that it likely does so by stimulating glycan chain polymerization. Overall, our results indicate that PBP accessory proteins play a central role in PG biogenesis and, like the PBPs they work with, these factors are attractive targets for antibiotic development.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Datsenko, K. A., & Wanner, B. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120163297 · Cited by 14,782
The Pfam Protein Families Database
Bateman, A. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.1.276 · Cited by 14,000
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
The Pfam protein families database
Finn, R. D., Tate, J., Mistry, J., Coggill, P. C., Sammut, S. J., Hotz, H.-R., Ceric, G., Forslund, K., Eddy, S. R., Sonnhammer, E. L. L., et al. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm960 · Cited by 1,845
Mechanism of Assembly of the Outer Membrane of Salmonella typhimurium
Osborn, M. J., Gander, J. E., Parisi, E., & Carson, J. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45127-2 · Cited by 1,568
The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
Sauvage, E., Kerff, F., Terrak, M., Ayala, J. A., & Charlier, P. (2008) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2008.00105.x · Cited by 1,317
de Boer, P. A. J., Crossley, R. E., & Rothfield, L. I. (1989) · Cell · doi:10.1016/0092-8674(89)90586-2 · Cited by 776
Taubes, G. (2008) · Science · doi:10.1126/science.321.5887.356 · Cited by 751
Mossessova, E., & Lima, C. D. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)80326-3 · Cited by 704
Cited by
Regulation of peptidoglycan synthesis and remodelling
Egan, A. J. F., Errington, J., & Vollmer, W. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-0366-3 · Cited by 592
Physical properties of the bacterial outer membrane
Sun, J., Rutherford, S. T., Silhavy, T. J., & Huang, K. C. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00638-0 · Cited by 404
The physiology of bacterial cell division
Egan, A. J. F., & Vollmer, W. (2013) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2012.06818.x · Cited by 320
Systems-level antimicrobial drug and drug synergy discovery
Roemer, T., & Boone, C. (2013) · Nature Chemical Biology · doi:10.1038/nchembio.1205 · Cited by 159
Navarro, P. P., Vettiger, A., Ananda, V. Y., Llopis, P. M., Allolio, C., Bernhardt, T. G., & Chao, L. H. (2022) · Nature Microbiology · doi:10.1038/s41564-022-01210-z · Cited by 102
Self-citation
Metadata
- Authors
- Paradis-Bleau, C., Markovski, M., Uehara, T., Lupoli, T. J., Walker, S., Kahne, D. E., & Bernhardt, T. G.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2010-12
- Volume
- 143
- Issue
- 7
- Pages
- 1110-1120
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2095480650
- Cited by
- 313
- References
- 48
- FWCI
- 10.23
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Lipoprotein Cofactors Located in the Outer Membrane Activate Bacterial Cell Wall Polymerases. https://app.cochat.ai/papers/10.1016/j.cell.2010.11.037
- The paper
- Paradis-Bleau, C., Markovski, M., Uehara, T., Lupoli, T. J., Walker, S., Kahne, D. E., & Bernhardt, T. G. (2010). Lipoprotein Cofactors Located in the Outer Membrane Activate Bacterial Cell Wall Polymerases. Cell. https://doi.org/10.1016/j.cell.2010.11.037
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
