The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2
Open accessAbstract
Ubiquitin chains of different topologies trigger distinct functional consequences, including protein degradation and reorganization of complexes. The assembly of most ubiquitin chains is promoted by E2s, yet how these enzymes achieve linkage specificity is poorly understood. We have discovered that the K11-specific Ube2S orients the donor ubiquitin through an essential non-covalent interaction that occurs in addition to the thioester bond at the E2 active site. The E2-donor ubiquitin complex transiently recognizes the acceptor ubiquitin, primarily through electrostatic interactions. The recognition of the acceptor ubiquitin surface around Lys11, but not around other lysines, generates a catalytically competent active site, which is composed of residues of both Ube2S and ubiquitin. Our studies suggest that monomeric E2s promote linkage-specific ubiquitin chain formation through substrate-assisted catalysis.
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Cited by
Komander, D., & Rape, M. (2012) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060310-170328 · Cited by 3,679
Swatek, K. N., & Komander, D. (2016) · Cell Research · doi:10.1038/cr.2016.39 · Cited by 2,081
Ubiquitin ligases: structure, function, and regulation
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The increasing complexity of the ubiquitin code
Yau, R., & Rape, M. (2016) · Nature Cell Biology · doi:10.1038/ncb3358 · Cited by 1,054
Breaking the chains: deubiquitylating enzyme specificity begets function
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Metadata
- Authors
- Wickliffe, K. E., Lorenz, S., Wemmer, D. E., Kuriyan, J., & Rape, M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2011-03
- Volume
- 144
- Issue
- 5
- Pages
- 769-781
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1970554372
- Cited by
- 279
- References
- 44
- FWCI
- 11.52
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wickliffe, K. E., Lorenz, S., Wemmer, D. E., Kuriyan, J., & Rape, M. (2011). The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2. Cell. https://doi.org/10.1016/j.cell.2011.01.035
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
