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Selective Ribosome Profiling Reveals the Cotranslational Chaperone Action of Trigger Factor In Vivo

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Oh, Eugene, Becker, Annemarie H., Sandikci, Arzu, Huber, Damon, Chaba, Rachna, Gloge, Felix, Nichols, Robert J., Typas, Athanasios, Gross, Carol A., Kramer, Günter, Weissman, Jonathan S., Bukau, Bernd·2011·Cell
DOI 10.1016/j.cell.2011.10.044
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Abstract

SUMMARY As nascent polypeptides exit ribosomes, they are engaged by a series of processing, targeting and folding factors. Here we present a selective ribosome profiling strategy that enables global monitoring of when these factors engage polypeptides in the complex cellular environment. Studies of the Escherichia coli chaperone Trigger Factor (TF) reveal that, while TF can interact with many polypeptides, β-barrel outer membrane proteins are the most prominent substrates. Loss of TF leads to broad outer membrane defects and premature, cotranslational protein translocation. While in vitro studies suggested that TF is prebound to ribosomes waiting for polypeptides to emerge from the exit channel, we find that in vivo TF engages ribosomes only after ~100 amino acids are translated. Moreover, excess TF interferes with cotrantslational removal of the N-terminal formyl methionine. Our studies support a triaging model in which proper protein biogenesis relies on the fine-tuned, sequential engagement of processing, targeting ad folding factors.

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Metadata

Authors
Oh, E., Becker, A. H., Sandikci, A., Huber, D., Chaba, R., Gloge, F., Nichols, R. J., Typas, A., Gross, C. A., Kramer, G., Weissman, J. S., & Bukau, B.
Venue
Cell
Publisher
Elsevier BV
Published
2011-12
Volume
147
Issue
6
Pages
1295-1308
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2011.10.044
OpenAlex
W2108926259
Cited by
453
References
7
FWCI
10.58
Type
journal article
Language
en
Open access
bronze
Licence

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CoChat. (2026). Citation check: Selective Ribosome Profiling Reveals the Cotranslational Chaperone Action of Trigger Factor In Vivo. https://app.cochat.ai/papers/10.1016/j.cell.2011.10.044
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Oh, E., Becker, A. H., Sandikci, A., Huber, D., Chaba, R., Gloge, F., Nichols, R. J., Typas, A., Gross, C. A., Kramer, G., Weissman, J. S., & Bukau, B. (2011). Selective Ribosome Profiling Reveals the Cotranslational Chaperone Action of Trigger Factor In Vivo. Cell. https://doi.org/10.1016/j.cell.2011.10.044

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