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A Subpopulation of Adult Skeletal Muscle Stem Cells Retains All Template DNA Strands after Cell Division
Open accessAbstract
Satellite cells are adult skeletal muscle stem cells that are quiescent and constitute a poorly defined heterogeneous population. Using transgenic Tg:Pax7-nGFP mice, we show that Pax7-nGFP(Hi) cells are less primed for commitment and have a lower metabolic status and delayed first mitosis compared to Pax7-nGFP(Lo) cells. Pax7-nGFP(Hi) can give rise to Pax7-nGFP(Lo) cells after serial transplantations. Proliferating Pax7-nGFP(Hi) cells exhibit lower metabolic activity, and the majority performs asymmetric DNA segregation during cell division, wherein daughter cells retaining template DNA strands express stem cell markers. Using chromosome orientation-fluorescence in situ hybridization, we demonstrate that all chromatids segregate asymmetrically, whereas Pax7-nGFP(Lo) cells perform random DNA segregation. Therefore, quiescent Pax7-nGFP(Hi) cells represent a reversible dormant stem cell state, and during muscle regeneration, Pax7-nGFP(Hi) cells generate distinct daughter cell fates by asymmetrically segregating template DNA strands to the stem cell. These findings provide major insights into the biology of stem cells that segregate DNA asymmetrically.
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“There is mounting evidence of asymmetric chromosome segregation in stem cells such as MuSCs17,18,129, ISCs20,130,131 and NSCs132.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [129]
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“In muscle, MuSCs also exist as a heterogeneous quiescent population in adult mice30–33, with changes in heterogeneity with ageing32,34,35.”
From Ageing and rejuvenation of tissue stem cells and their niches (2023) · cites this paper as [31]
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Satellite cell expansion is mediated by P-eIF2α dependent Tacc3 translation
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Metadata
- Authors
- Rocheteau, P., Gayraud-Morel, B., Siegl-Cachedenier, I., Blasco, M. A., & Tajbakhsh, S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Tajbakhsh, Shahragim
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 148
- Issue
- 1-2
- Pages
- 112-125
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2037334278
- Cited by
- 510
- References
- 57
- Citations by year
- 2026: 16 · 2025: 17 · 2024: 34 · 2023: 45 · 2022: 33 · 2021: 29 · 2020: 31 · 2019: 33 · 2018: 51 · 2017: 41 · 2016: 30 · 2015: 38 · 2014: 39 · 2013: 40 · 2012: 31
- FWCI
- 19.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 563 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-06-BLAN-0039)
- Centre National de la Recherche Scientifique
- Ministerio de Ciencia e Innovación
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- skeletal muscle stem cells, satellite cells, Pax7, stem cell quiescence, cell fate determination, muscle regeneration, cellular metabolism, first mitosis, stem cell heterogeneity, serial transplantation
- MeSH terms
- Animals, Cell Division, Female, Mice, Transgenic, Templates, Genetic, Muscle, Skeletal, Chromosome Segregation, Satellite Cells, Skeletal Muscle, Mice, PAX7 Transcription Factor, Adult Stem Cells
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