The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens
Open accessAbstract
SUMMARY Optical imaging of the dynamics of living specimens involves tradeoffs between spatial resolution, temporal resolution, and phototoxicity, made more difficult in three-dimensions. Here, however, we report that rapid 3D dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultra-thin planar illumination produced by scanned Bessel beams with superresolution structured illumination microscopy. We demonstrate in vivo karyotyping of chromosomes during mitosis and identify different dynamics for the actin cytoskeleton at the dorsal and ventral surfaces of fibroblasts. Compared to spinning disk confocal microscopy, we demonstrate substantially reduced photodamage when imaging rapid morphological changes in D. discoideum cells, as well as improved contrast and resolution at depth within developing C. elegans embryos. Bessel beam structured plane illumination thus promises new insights into complex biological phenomena that require 4D subcellular spatiotemporal detail in either a single or multicellular context.
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Cites
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F. (2006) · Science · doi:10.1126/science.1127344 · Cited by 8,772
Self-citationGustafsson, M. G. L. (2000) · Journal of Microscopy · doi:10.1046/j.1365-2818.2000.00710.x · Cited by 3,786
Gustafsson, M. G. L., Shao, L., Carlton, P. M., Wang, C. J. R., Golubovskaya, I. N., Cande, W. Z., Agard, D. A., & Sedat, J. W. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.120345 · Cited by 1,557
Self-citationA guide to super-resolution fluorescence microscopy
Schermelleh, L., Heintzmann, R., & Leonhardt, H. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201002018 · Cited by 1,255
Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain
Dodt, H.-U., Leischner, U., Schierloh, A., Jährling, N., Mauch, C. P., Deininger, K., Deussing, J. M., Eder, M., Zieglgänsberger, W., & Becker, K. (2007) · Nature Methods · doi:10.1038/nmeth1036 · Cited by 1,238
Light Microscopy Techniques for Live Cell Imaging
Stephens, D. J., & Allan, V. J. (2003) · Science · doi:10.1126/science.1082160 · Cited by 1,179
Method of obtaining optical sectioning by using structured light in a conventional microscope
Neil, M. A. A., Juškaitis, R., & Wilson, T. (1997) · Optics Letters · doi:10.1364/ol.22.001905 · Cited by 1,143
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain
Hama, H., Kurokawa, H., Kawano, H., Ando, R., Shimogori, T., Noda, H., Fukami, K., Sakaue-Sawano, A., & Miyawaki, A. (2011) · Nature Neuroscience · doi:10.1038/nn.2928 · Cited by 1,142
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
Planchon, T. A., Gao, L., Milkie, D. E., Davidson, M. W., Galbraith, J. A., Galbraith, C. G., & Betzig, E. (2011) · Nature Methods · doi:10.1038/nmeth.1586 · Cited by 1,112
Self-citationTotal Internal Reflection Fluorescence Microscopy in Cell Biology
Axelrod, D. (2001) · Traffic · doi:10.1034/j.1600-0854.2001.21104.x · Cited by 959
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Self-citationSingle-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
Chen, J., Zhang, Z., Li, L., Chen, B.-C., Revyakin, A., Hajj, B., Legant, W., Dahan, M., Lionnet, T., Betzig, E., et al. (2014) · Cell · doi:10.1016/j.cell.2014.01.062 · Cited by 602
Self-citationLight-induced cell damage in live-cell super-resolution microscopy
Wäldchen, S., Lehmann, J., Klein, T., van de Linde, S., & Sauer, M. (2015) · Scientific Reports · doi:10.1038/srep15348 · Cited by 536
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Dean, K. M., & Palmer, A. E. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1556 · Cited by 512
Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs)
Theillet, F.-X., Binolfi, A., Frembgen-Kesner, T., Hingorani, K., Sarkar, M., Kyne, C., Li, C., Crowley, P. B., Gierasch, L., Pielak, G. J., et al. (2014) · Chemical Reviews · doi:10.1021/cr400695p · Cited by 503
Metadata
- Authors
- Gao, L., Shao, L., Higgins, C. D., Poulton, J. S., Peifer, M., Davidson, M. W., Wu, X., Goldstein, B., & Betzig, E.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-12
- Volume
- 151
- Issue
- 6
- Pages
- 1370-1385
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2144613560
- Cited by
- 320
- References
- 30
- FWCI
- 16.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gao, L., Shao, L., Higgins, C. D., Poulton, J. S., Peifer, M., Davidson, M. W., Wu, X., Goldstein, B., & Betzig, E. (2012). Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens. Cell. https://doi.org/10.1016/j.cell.2012.10.008
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