The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Probing the Stochastic, Motor-Driven Properties of the Cytoplasm Using Force Spectrum Microscopy
Open accessAbstract
SUMMARY Molecular motors in cells typically produce highly directed motion; however, the aggregate, incoherent effect of all active processes also creates randomly fluctuating forces, which drive diffusive-like, non-thermal motion. Here we introduce force-spectrum-microscopy (FSM) to directly quantify random forces within the cytoplasm of cells and thereby probe stochastic motor activity. This technique combines measurements of the random motion of probe particles with independent micromechanical measurements of the cytoplasm to quantify the spectrum of force fluctuations. Using FSM, we show that force fluctuations substantially enhance intracellular movement of small and large components. The fluctuations are three times larger in malignant cells than in their benign counterparts. We further demonstrate that vimentin acts globally to anchor organelles against randomly fluctuating forces in the cytoplasm, with no effect on their magnitude. Thus, FSM has broad applications for understanding the cytoplasm and its intracellular processes in relation to cell physiology in healthy and diseased states.
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Sherr, C. J. (1996) · Science · doi:10.1126/science.274.5293.1672 · Cited by 5,379
Mechanics of Motor Proteins and the Cytoskeleton
Howard, & Clark (2002) · Applied Mechanics Reviews · doi:10.1115/1.1451234 · Cited by 2,909 · cited 2×
“Myosin II is a nonprocessive motor protein that binds to actin filaments and undergoes a power stroke upon ATP hydrolysis (Howard, 2001).”
The Molecular Motor Toolbox for Intracellular Transport
Vale, R. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00111-9 · Cited by 1,982
Nanomechanical analysis of cells from cancer patients
Cross, S. E., Jin, Y.-S., Rao, J., & Gimzewski, J. K. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.388 · Cited by 1,908
Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
Grashoff, C., Hoffman, B. D., Brenner, M. D., Zhou, R., Parsons, M., Yang, M. T., McLean, M. A., Sligar, S. G., Chen, C. S., Ha, T., et al. (2010) · Nature · doi:10.1038/nature09198 · Cited by 1,541
Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
Straight, A. F., Cheung, A., Limouze, J., Chen, I., Westwood, N. J., Sellers, J. R., & Mitchison, T. J. (2003) · Science · doi:10.1126/science.1081412 · Cited by 1,412
Scaling the Microrheology of Living Cells
Fabry, B., Maksym, G. N., Butler, J. P., Glogauer, M., Navajas, D., & Fredberg, J. J. (2001) · Physical Review Letters · doi:10.1103/physrevlett.87.148102 · Cited by 1,261
Forces in Tissue Morphogenesis and Patterning
Heisenberg, C.-P., & Bellaïche, Y. (2013) · Cell · doi:10.1016/j.cell.2013.05.008 · Cited by 1,216
Luby-Phelps, K. (1999) · International Review of Cytology · doi:10.1016/s0074-7696(08)60527-6 · Cited by 1,149
The nanomechanical signature of breast cancer
Plodinec, M., Loparic, M., Monnier, C. A., Obermann, E. C., Zanetti-Dallenbach, R., Oertle, P., Hyotyla, J. T., Aebi, U., Bentires-Alj, M., Lim, R. Y. H., et al. (2012) · Nature Nanotechnology · doi:10.1038/nnano.2012.167 · Cited by 1,102
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Chaudhuri, O., Cooper-White, J., Janmey, P. A., Mooney, D. J., & Shenoy, V. B. (2020) · Nature · doi:10.1038/s41586-020-2612-2 · Cited by 2,224
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 747
Active matter at the interface between materials science and cell biology
Needleman, D., & Dogic, Z. (2017) · Nature Reviews Materials · doi:10.1038/natrevmats.2017.48 · Cited by 649
Munder, M. C., Midtvedt, D., Franzmann, T., Nüske, E., Otto, O., Herbig, M., Ulbricht, E., Müller, P., Taubenberger, A., Maharana, S., et al. (2016) · eLife · doi:10.7554/elife.09347 · Cited by 473
Nixon-Abell, J., Obara, C. J., Weigel, A. V., Li, D., Legant, W. R., Xu, C. S., Pasolli, H. A., Harvey, K., Hess, H. F., Betzig, E., et al. (2016) · Science · doi:10.1126/science.aaf3928 · Cited by 455
Self-citation
Metadata
- Authors
- Guo, M., Ehrlicher, A. J., Jensen, M. H., Renz, M., Moore, J. R., Goldman, R. D., Lippincott-Schwartz, J., Mackintosh, F. C., & Weitz, D. A.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2014-08
- Volume
- 158
- Issue
- 4
- Pages
- 822-832
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2133660324
- Cited by
- 566
- References
- 53
- FWCI
- 20.31
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Guo, M., Ehrlicher, A. J., Jensen, M. H., Renz, M., Moore, J. R., Goldman, R. D., Lippincott-Schwartz, J., Mackintosh, F. C., & Weitz, D. A. (2014). Probing the Stochastic, Motor-Driven Properties of the Cytoplasm Using Force Spectrum Microscopy. Cell. https://doi.org/10.1016/j.cell.2014.06.051
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