The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Coexisting Liquid Phases Underlie Nucleolar Subcompartments
Open accessAbstract
Summary The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles that appear to assemble through phase separation of their molecular components. However, many such RNP bodies contain internal sub-compartments, and the mechanism of their formation remains unclear. Here, we combine in vivo and in vitro studies, together with computational modeling, to show that sub-compartments within the nucleolus represent distinct, coexisting liquid phases. Consistent with their in vivo immiscibility, purified nucleolar proteins phase separate into droplets containing distinct non-coalescing phases that are remarkably similar to nucleoli in vivo. This layered droplet organization is caused by differences in the biophysical properties of the phases – particularly droplet surface tension – which arises from sequence-encoded features of their macromolecular components. These results suggest that phase separation can give rise to multilayered liquids that may facilitate sequential RNA processing reactions in a variety of RNP bodies.
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Elbaum-Garfinkle, S., Kim, Y., Szczepaniak, K., Chen, C. C.-H., Eckmann, C. R., Myong, S., & Brangwynne, C. P. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1504822112 · Cited by 1,278
Self-citationPederson, T. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000638 · Cited by 455
Cited by
Alberti, S., Gladfelter, A., & Mittag, T. (2019) · Cell · doi:10.1016/j.cell.2018.12.035 · Cited by 2,856
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Sabari, B. R., Dall’Agnese, A., Boija, A., Klein, I. A., Coffey, E. L., Shrinivas, K., Abraham, B. J., Hannett, N. M., Zamudio, A. V., Manteiga, J. C., et al. (2018) · Science · doi:10.1126/science.aar3958 · Cited by 2,595
A Phase Separation Model for Transcriptional Control
Hnisz, D., Shrinivas, K., Young, R. A., Chakraborty, A. K., & Sharp, P. A. (2017) · Cell · doi:10.1016/j.cell.2017.02.007 · Cited by 1,753
The nucleolus as a multiphase liquid condensate
Lafontaine, D. L. J., Riback, J. A., Bascetin, R., & Brangwynne, C. P. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0272-6 · Cited by 1,051
Self-citationKi-67: more than a proliferation marker
Sun, X., & Kaufman, P. D. (2018) · Chromosoma · doi:10.1007/s00412-018-0659-8 · Cited by 963
Metadata
- Authors
- Feric, M., Vaidya, N., Harmon, T. S., Mitrea, D. M., Zhu, L., Richardson, T. M., Kriwacki, R. W., Pappu, R. V., & Brangwynne, C. P.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-06
- Volume
- 165
- Issue
- 7
- Pages
- 1686-1697
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2402791934
- Cited by
- 1,945
- References
- 2
- FWCI
- 59.89
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Feric, M., Vaidya, N., Harmon, T. S., Mitrea, D. M., Zhu, L., Richardson, T. M., Kriwacki, R. W., Pappu, R. V., & Brangwynne, C. P. (2016). Coexisting Liquid Phases Underlie Nucleolar Subcompartments. Cell. https://doi.org/10.1016/j.cell.2016.04.047
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