The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
The DEAD-Box Protein Dhh1p Couples mRNA Decay and Translation by Monitoring Codon Optimality
Open accessAbstract
Summary A major determinant of mRNA half-life is the codon-dependent rate of translational elongation. How the processes of translational elongation and mRNA decay communicate is unclear. Here we establish that the DEAD-box protein Dhh1p is a sensor of codon optimality that targets an mRNA for decay. First, we find mRNAs whose translation elongation rate is slowed by inclusion of nonoptimal codons are specifically degraded in a Dhh1p-dependent manner. Biochemical experiments show Dhh1p is preferentially associated with mRNAs with suboptimal codon choice. We find these effects on mRNA decay are sensitive to the number of slow moving ribosomes on an mRNA. Moreover, we find Dhh1p overexpression leads to the accumulation of ribosomes specifically on mRNAs (and even codons) of low codon optimality. Lastly, Dhh1p physically interacts with ribosomes in vivo. Together, these data argue that Dhh1p is a sensor for ribosome speed, targeting an mRNA for repression and subsequent decay.
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Cites
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Codon Optimality Is a Major Determinant of mRNA Stability
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“These data agree with our previous findings that codon optimality is a major determinant of mRNA stability (Presnyak et al., 2015).”
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Metadata
- Authors
- Radhakrishnan, A., Chen, Y.-H., Martin, S., Alhusaini, N., Green, R., & Coller, J.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 167
- Issue
- 1
- Pages
- 122-132.e9
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2520264800
- Cited by
- 289
- References
- 12
- FWCI
- 14.75
- Type
- journal article
- Language
- en
- Open access
- bronze
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