Cited by 522Cell Image Analysis TechniquesAdvanced Fluorescence Microscopy TechniquesDevelopmental Biology and Gene Regulation

In toto imaging and reconstruction of post-implantation mouse development at the single-cell level

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McDole, Katie, Guignard, Léo, Amat, Fernando, Berger, Andrew, Malandain, Grégoire, Royer, Loïc A., Turaga, Srinivas C., Branson, Kristin +1 more·2018·Cell

Abstract

The mouse embryo has long been central to the study of mammalian development; however, elucidating the cell behaviors governing gastrulation and the formation of tissues and organs remains a fundamental challenge. A major obstacle is the lack of live imaging and image analysis technologies capable of systematically following cellular dynamics across the developing embryo. We developed a light-sheet microscope that adapts itself to the dramatic changes in size, shape, and optical properties of the post-implantation mouse embryo and captures its development from gastrulation to early organogenesis at the cellular level. We furthermore developed a computational framework for reconstructing long-term cell tracks, cell divisions, dynamic fate maps, and maps of tissue morphogenesis across the entire embryo. By jointly analyzing cellular dynamics in multiple embryos registered in space and time, we built a dynamic atlas of post-implantation mouse development that, together with our microscopy and computational methods, is provided as a resource. VIDEO ABSTRACT.

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Crossref · 10.1016/j.cell.2018.09.031

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  • Classical imaging technologies (including live-cell), newer structural imaging technologies (such as cryoelectron tomography and soft X-ray tomography), and novel spatial omics technologies offer opportunities to model biomolecules and functions across scales.

    “Here, classical imaging technologies,65,100,101 including live-cell, and newer structural imaging technologies, such as cryoelectron tomography and soft X-ray tomography,66,102,103 as well as novel spatial omics technologies,104,105 offer opportunities to model biomolecules and functions across scales.”

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Authors
McDole, K., Guignard, L., Amat, F., Berger, A., Malandain, G., Royer, L. A., Turaga, S. C., Branson, K., & Keller, P. J.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
  • McDole, Katie
  • Guignard, Léo
  • Keller, Philipp J.
Venue
Cell
Publisher
Elsevier BV
Published
2018-10
Volume
175
Issue
3
Pages
859-876.e33
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2896103138
Cited by
522
References
51
Citations by year
2026: 55 · 2025: 72 · 2024: 67 · 2023: 48 · 2022: 61 · 2021: 77 · 2020: 77 · 2019: 51 · 2018: 13
FWCI
28.46
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • French National Research Agency (ANR-14-CE11-0013)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
light-sheet microscopy, cell tracking, tissue morphogenesis, gastrulation, organogenesis, developmental atlas, live embryo imaging
MeSH terms
Animals, Mice, Inbred C57BL, Models, Statistical, Cell Lineage, Organogenesis, Mice, Gastrulation, Single-Cell Analysis, Optical Imaging

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