The DOI matches its Crossref record; 108 of the 108 references it cites were found in a registry or an index.
Organization of Chromatin by Intrinsic and Regulated Phase Separation
Open accessAbstract
SUMMARY Eukaryotic chromatin is highly condensed yet dynamically accessible to regulation, and organized into subdomains. We demonstrate that reconstituted chromatin undergoes histone tail-driven liquid-liquid phase separation (LLPS) in physiologic salt and when microinjected into cell nuclei, producing dense and dynamic droplets. Linker histone H1 and internucleosome linker lengths shared across eukaryotes promote phase separation of chromatin, tune droplet properties, and coordinate to form condensates of consistent density, in manners that parallel chromatin behaviors in cells. Histone acetylation by p300 antagonizes chromatin phase separation, dissolving droplets in vitro and decreasing droplet formation in nuclei. In the presence of multi-bromodomain proteins, such as BRD4, highly acetylated chromatin forms a new phase separated state, with droplets of distinct physical properties, which can be immiscible with unmodified chromatin droplets, mimicking nuclear chromatin subdomains. Our data suggest a framework, based on intrinsic phase separation of the chromatin polymer, for understanding organization and regulation of eukaryotic genomes.
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Metadata
- Authors
- Gibson, B. A., Doolittle, L. K., Schneider, M. W. G., Jensen, L. E., Gamarra, N., Henry, L., Gerlich, D. W., Redding, S., & Rosen, M. K.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2019-10
- Volume
- 179
- Issue
- 2
- Pages
- 470-484.e21
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2973290848
- Cited by
- 1,072
- References
- 108
- FWCI
- 36.31
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gibson, B. A., Doolittle, L. K., Schneider, M. W. G., Jensen, L. E., Gamarra, N., Henry, L., Gerlich, D. W., Redding, S., & Rosen, M. K. (2019). Organization of Chromatin by Intrinsic and Regulated Phase Separation. Cell. https://doi.org/10.1016/j.cell.2019.08.037
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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