The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions
Open accessAbstract
Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of the extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development prior to epiblast commitment. Similarly, 2i ESC cultures were heterogeneous and contained a Hex-positive fraction primed to differentiate into trophoblast and extraembryonic endoderm. Single Hex-positive ESCs coexpressed epiblast and extraembryonic genes and contributed to all lineages in chimeras. The cytokine LIF, necessary for ESC self-renewal, supported the expansion of this population but did not directly support Nanog-positive epiblast-like ESCs. Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants.
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Cites
The ground state of embryonic stem cell self-renewal
Ying, Q.-L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., & Smith, A. (2008) · Nature · doi:10.1038/nature06968 · Cited by 3,442
Self-citationConversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504
Chazaud, C., Yamanaka, Y., Pawson, T., & Rossant, J. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.02.020 · Cited by 898
Stat3 and c-Myc genome-wide promoter occupancy in embryonic stem cells
Kidder, B. L., Yang, J., & Palmer, S. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003932 · Cited by 184
Cited by
Formative pluripotency: the executive phase in a developmental continuum
Smith, A. (2017) · Development · doi:10.1242/dev.142679 · Cited by 474
The emergent landscape of the mouse gut endoderm at single-cell resolution
Nowotschin, S., Setty, M., Kuo, Y.-Y., Liu, V., Garg, V., Sharma, R., Simon, C. S., Saiz, N., Gardner, R., Boutet, S. C., et al. (2019) · Nature · doi:10.1038/s41586-019-1127-1 · Cited by 383
Athanasouli, P., Balli, M., De Jaime-Soguero, A., Boel, A., Papanikolaou, S., van der Veer, B. K., Janiszewski, A., Vanhessche, T., Francis, A., El Laithy, Y., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36914-1 · Cited by 31
Metadata
- Authors
- Morgani, S. M., Canham, M. A., Nichols, J., Sharov, A. A., Migueles, R. P., Ko, M. S. H., & Brickman, J. M.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 3
- Issue
- 6
- Pages
- 1945-1957
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2035203405
- Cited by
- 231
- References
- 4
- FWCI
- 10.89
- Type
- journal article
- Language
- en
- Open access
- gold
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- Morgani, S. M., Canham, M. A., Nichols, J., Sharov, A. A., Migueles, R. P., Ko, M. S. H., & Brickman, J. M. (2013). Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions. Cell Reports. https://doi.org/10.1016/j.celrep.2013.04.034
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