The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions
Open accessAbstract
Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of the extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development prior to epiblast commitment. Similarly, 2i ESC cultures were heterogeneous and contained a Hex-positive fraction primed to differentiate into trophoblast and extraembryonic endoderm. Single Hex-positive ESCs coexpressed epiblast and extraembryonic genes and contributed to all lineages in chimeras. The cytokine LIF, necessary for ESC self-renewal, supported the expansion of this population but did not directly support Nanog-positive epiblast-like ESCs. Thus, 2i and LIF support a totipotent state comparable to early embryonic cells that coexpress embryonic and extraembryonic determinants.
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Cites
The ground state of embryonic stem cell self-renewal
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Self-citationConversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
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Stat3 and c-Myc genome-wide promoter occupancy in embryonic stem cells
Kidder, B. L., Yang, J., & Palmer, S. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003932 · Cited by 184
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Yang, Y., Liu, B., Xu, J., Wang, J., Wu, J., Shi, C., Xu, Y., Dong, J., Wang, C., Lai, W., et al. (2017) · Cell · doi:10.1016/j.cell.2017.02.005 · Cited by 502
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De Los Angeles, A., Ferrari, F., Xi, R., Fujiwara, Y., Benvenisty, N., Deng, H., Hochedlinger, K., Jaenisch, R., Lee, S., Leitch, H. G., et al. (2015) · Nature · doi:10.1038/nature15515 · Cited by 396
The emergent landscape of the mouse gut endoderm at single-cell resolution
Nowotschin, S., Setty, M., Kuo, Y.-Y., Liu, V., Garg, V., Sharma, R., Simon, C. S., Saiz, N., Gardner, R., Boutet, S. C., et al. (2019) · Nature · doi:10.1038/s41586-019-1127-1 · Cited by 383
Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly
Ishiuchi, T., Enriquez-Gasca, R., Mizutani, E., Bošković, A., Ziegler-Birling, C., Rodriguez-Terrones, D., Wakayama, T., Vaquerizas, J. M., & Torres-Padilla, M.-E. (2015) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.3066 · Cited by 335
Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures
Li, R., Zhong, C., Yu, Y., Liu, H., Sakurai, M., Yu, L., Min, Z., Shi, L., Wei, Y., Takahashi, Y., et al. (2019) · Cell · doi:10.1016/j.cell.2019.09.029 · Cited by 267
Athanasouli, P., Balli, M., De Jaime-Soguero, A., Boel, A., Papanikolaou, S., van der Veer, B. K., Janiszewski, A., Francis, A., El Laithy, Y., Lo Nigro, A., et al. (2022) · doi:10.1101/2022.05.18.492419 · Cited by 31
Athanasouli, P., Balli, M., De Jaime-Soguero, A., Boel, A., Papanikolaou, S., van der Veer, B. K., Janiszewski, A., Vanhessche, T., Francis, A., El Laithy, Y., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36914-1 · Cited by 31
Metadata
- Authors
- Morgani, S. M., Canham, M. A., Nichols, J., Sharov, A. A., Migueles, R. P., Ko, M. S. H., & Brickman, J. M.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 3
- Issue
- 6
- Pages
- 1945-1957
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2035203405
- Cited by
- 231
- References
- 4
- FWCI
- 10.89
- Type
- journal article
- Language
- en
- Open access
- gold
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
