The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Widespread Inhibition of Posttranscriptional Splicing Shapes the Cellular Transcriptome following Heat Shock
Open accessAbstract
During heat shock and other proteotoxic stresses, cells regulate multiple steps in gene expression in order to globally repress protein synthesis and selectively upregulate stress response proteins. Splicing of several mRNAs is known to be inhibited during heat stress, often meditated by SRp38, but the extent and specificity of this effect have remained unclear. Here, we examined splicing regulation genome-wide during heat shock in mouse fibroblasts. We observed widespread retention of introns in transcripts from ∼1,700 genes, which were enriched for tRNA synthetase, nuclear pore, and spliceosome functions. Transcripts with retained introns were largely nuclear and untranslated. However, a group of 580+ genes biased for oxidation reduction and protein folding functions continued to be efficiently spliced. Interestingly, these unaffected transcripts are mostly cotranscriptionally spliced under both normal and stress conditions, whereas splicing-inhibited transcripts are mostly spliced posttranscriptionally. Altogether, our data demonstrate widespread repression of splicing in the mammalian heat stress response, disproportionately affecting posttranscriptionally spliced genes.
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Cites
Alternative isoform regulation in human tissue transcriptomes
Wang, E. T., Sandberg, R., Luo, S., Khrebtukova, I., Zhang, L., Mayr, C., Kingsmore, S. F., Schroth, G. P., & Burge, C. B. (2008) · Nature · doi:10.1038/nature07509 · Cited by 5,181
Self-citationMolecular mechanisms of translational control
Gebauer, F., & Hentze, M. W. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1488 · Cited by 991
Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells
Murray, J. I., Whitfield, M. L., Trinklein, N. D., Myers, R. M., Brown, P. O., & Botstein, D. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-11-0799 · Cited by 300
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Labbadia, J., & Morimoto, R. I. (2015) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060614-033955 · Cited by 1,472
Mammalian heat shock response and mechanisms underlying its genome-wide transcriptional regulation
Mahat, D. B., Salamanca, H. H., Duarte, F. M., Danko, C. G., & Lis, J. T. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.02.025 · Cited by 426
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Detained introns are a novel, widespread class of post-transcriptionally spliced introns
Boutz, P. L., Bhutkar, A., & Sharp, P. A. (2015) · Genes & Development · doi:10.1101/gad.247361.114 · Cited by 425
Intron retention as a component of regulated gene expression programs
Jacob, A. G., & Smith, C. W. J. (2017) · Human Genetics · doi:10.1007/s00439-017-1791-x · Cited by 334
Nuclear Retention of mRNA in Mammalian Tissues
Bahar Halpern, K., Caspi, I., Lemze, D., Levy, M., Landen, S., Elinav, E., Ulitsky, I., & Itzkovitz, S. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.11.036 · Cited by 258
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Comparative analysis reveals genomic features of stress-induced transcriptional readthrough
Vilborg, A., Sabath, N., Wiesel, Y., Nathans, J., Levy-Adam, F., Yario, T. A., Steitz, J. A., & Shalgi, R. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1711120114 · Cited by 131
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Tan, Z.-W., Fei, G., Paulo, J. A., Bellaousov, S., Martin, S. E. S., Duveau, D. Y., Thomas, C. J., Gygi, S. P., Boutz, P. L., & Walker, S. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa263 · Cited by 119
Stress Granules Contain Rbfox2 with Cell Cycle-related mRNAs
Park, C., Choi, S., Kim, Y.-E., Lee, S., Park, S.-H., Adelstein, R. S., Kawamoto, S., & Kim, K. K. (2017) · Scientific Reports · doi:10.1038/s41598-017-11651-w · Cited by 49
The fission yeast methyl phosphate capping enzyme Bmc1 guides 2′-O-methylation of the U6 snRNA
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Metadata
- Authors
- Shalgi, R., Hurt, J. A., Lindquist, S., & Burge, C. B.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2014-06
- Volume
- 7
- Issue
- 5
- Pages
- 1362-1370
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2078779750
- Cited by
- 191
- References
- 3
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- gold
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