Comparative analysis reveals genomic features of stress-induced transcriptional readthrough
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Significance: Cells and organisms live in constantly changing environments. Therefore, cells have evolved complex mechanisms to cope with physiological and environmental stresses. Many of these mechanisms involve transcriptional responses facilitating survival and adaptation. Recent evidence documents extensive transcriptional readthrough beyond annotated gene ends in response to stress, but the role and regulation of these downstream of gene-containing transcripts (DoGs) remain elusive. Here we report that induction of transcriptional readthrough is a hallmark of the mammalian stress response. We explore its causes and consequences in a genome-wide fashion, identifying thousands of readthrough transcripts that are induced in three different stress conditions. Our results suggest potential roles for this class of transcripts in the maintenance of open chromatin under stress.
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Transcriptional read-through is induced downstream of thousands of genes in response to different kinds of stresses.
“Moreover, transcriptional read‐through is induced downstream of thousands of genes in response to different kinds of stresses (Rutkowski et al, 2015; Vilborg et al, 2015; Vilborg et al, 2017; Cardiello et al, 2018; Hennig et al, 2018) and has been proposed to play an important role in the stress response by reinforcing the nuclear scaffold integrity after nuclear stress (Vilborg et al, 2015).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Vilborg et al, 2017
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RNA polymerase II speed: a key player in controlling and adapting transcriptome composition
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“…read-through is induced downstream of thousands of genes in response to different kinds of stresses (Rutkowski et al , 2015; Vilborg et al , 2015; Vilborg et al , 2017; Cardiello et al , 2018; Hennig et al , 2018) and has been proposed to play an important role in the stress response by…”
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- Authors
- Vilborg, A., Sabath, N., Wiesel, Y., Nathans, J., Levy-Adam, F., Yario, T. A., Steitz, J. A., & Shalgi, R.
- Affiliations
- Howard Hughes Medical Institute (US)
- Technion – Israel Institute of Technology (IL)
- Department of Biochemistry, Rappaport Faculty of Medicine, Technion–Israel Institute of Technology, Haifa 31096, Israel
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-09-19
- Volume
- 114
- Issue
- 40
- Pages
- E8362-E8371
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5635911
- OpenAlex
- W2755390119
- Cited by
- 131
- References
- 34
- Citations by year
- 2026: 13 · 2025: 10 · 2024: 20 · 2023: 16 · 2022: 20 · 2021: 19 · 2020: 10 · 2019: 9 · 2018: 13 · 2017: 1
- FWCI
- 3.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- California Institute of Technology
- National Institute of General Medical Sciences (R01GM026154)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- read-through transcription, stress response, heat shock, osmotic stress, oxidative stress, HSF1, antisense transcription, open chromatin, NIH3T3 cells, non-coding RNA
- MeSH terms
- Animals, Gene Expression Regulation, Osmotic Pressure, Transcription, Genetic, Genome, Oxidative Stress, Heat-Shock Response, Gene Expression Profiling, Genomics, NIH 3T3 Cells, Mice
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