The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Transcription Factors Modulate c-Fos Transcriptional Bursts
Open accessAbstract
Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)--especially in rapidly inducible systems--is largely unknown. Using fluorescence in situ hybridization and computational models, we quantified the transcriptional activity of the proto-oncogene c-Fos with single mRNA accuracy at individual endogenous alleles. We showed that, during MAPK induction, the TF concentration modulates the burst frequency of c-Fos, whereas other bursting parameters remain mostly unchanged. By using synthetic TFs with TALE DNA-binding domains, we systematically altered different aspects of these bursts. Specifically, we linked the polymerase initiation frequency to the strength of the transactivation domain and the burst duration to the TF lifetime on the promoter. Our results show how TFs and promoter binding domains collectively act to regulate different bursting parameters, offering a vast, evolutionarily tunable regulatory range for individual genes.
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Cites
Imaging individual mRNA molecules using multiple singly labeled probes
Raj, A., van den Bogaard, P., Rifkin, S. A., van Oudenaarden, A., & Tyagi, S. (2008) · Nature Methods · doi:10.1038/nmeth.1253 · Cited by 2,028
Stochastic mRNA Synthesis in Mammalian Cells
Raj, A., Peskin, C. S., Tranchina, D., Vargas, D. Y., & Tyagi, S. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040309 · Cited by 1,737
Visualization of Single RNA Transcripts in Situ
Femino, A. M., Fay, F. S., Fogarty, K., & Singer, R. H. (1998) · Science · doi:10.1126/science.280.5363.585 · Cited by 1,504
Mammalian genes are transcribed with widely different bursting kinetics
Suter, D. M., Molina, N., Gatfield, D., Schneider, K., Schibler, U., & Naef, F. (2011) · Science · doi:10.1126/science.1198817 · Cited by 927
Using Gene Expression Noise to Understand Gene Regulation
Munsky, B., Neuert, G., & van Oudenaarden, A. (2012) · Science · doi:10.1126/science.1216379 · Cited by 745
Self-citationSingle-RNA counting reveals alternative modes of gene expression in yeast
Zenklusen, D., Larson, D. R., & Singer, R. H. (2008) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1514 · Cited by 684
Cited by
Mechanisms regulating zygotic genome activation
Schulz, K. N., & Harrison, M. M. (2019) · Nature Reviews Genetics · doi:10.1038/s41576-018-0087-x · Cited by 555
Padovan-Merhar, O., Nair, G. P., Biaesch, A. G., Mayer, A., Scarfone, S., Foley, S. W., Wu, A. R., Churchman, L. S., Singh, A., & Raj, A. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.03.005 · Cited by 451
Enhancer Regulation of Transcriptional Bursting Parameters Revealed by Forced Chromatin Looping
Bartman, C. R., Hsu, S. C., Hsiung, C. C.-S., Raj, A., & Blobel, G. A. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.03.007 · Cited by 345
Nuclear Retention of mRNA in Mammalian Tissues
Bahar Halpern, K., Caspi, I., Lemze, D., Levy, M., Landen, S., Elinav, E., Ulitsky, I., & Itzkovitz, S. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.11.036 · Cited by 258
A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting
Tantale, K., Mueller, F., Kozulic-Pirher, A., Lesne, A., Victor, J.-M., Robert, M.-C., Capozi, S., Chouaib, R., Bäcker, V., Mateos-Langerak, J., et al. (2016) · Nature Communications · doi:10.1038/ncomms12248 · Cited by 300
Self-citation
Metadata
- Authors
- Senecal, A., Munsky, B., Proux, F., Ly, N., Braye, F. E., Zimmer, C., Mueller, F., & Darzacq, X.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2014-07
- Volume
- 8
- Issue
- 1
- Pages
- 75-83
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2075865654
- Cited by
- 281
- References
- 6
- FWCI
- 7.42
- Type
- journal article
- Language
- en
- Open access
- gold
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