The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting
Open accessAbstract
Live-cell imaging has revealed unexpected features of gene expression. Here using improved single-molecule RNA microscopy, we show that synthesis of HIV-1 RNA is achieved by groups of closely spaced polymerases, termed convoys, as opposed to single isolated enzymes. Convoys arise by a Mediator-dependent reinitiation mechanism, which generates a transient but rapid succession of polymerases initiating and escaping the promoter. During elongation, polymerases are spaced by few hundred nucleotides, and physical modelling suggests that DNA torsional stress may maintain polymerase spacing. We additionally observe that the HIV-1 promoter displays stochastic fluctuations on two time scales, which we refer to as multi-scale bursting. Each time scale is regulated independently: Mediator controls minute-scale fluctuation (convoys), while TBP-TATA-box interaction controls sub-hour fluctuations (long permissive/non-permissive periods). A cellular promoter also produces polymerase convoys and displays multi-scale bursting. We propose that slow, TBP-dependent fluctuations are important for phenotypic variability of single cells.
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smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability
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Self-citationThe Intersection of Epigenetics and Metabolism in Trained Immunity
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Intracellular mRNA transport and localized translation
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Metadata
- Authors
- Tantale, K., Mueller, F., Kozulic-Pirher, A., Lesne, A., Victor, J.-M., Robert, M.-C., Capozi, S., Chouaib, R., Bäcker, V., Mateos-Langerak, J., Darzacq, X., Zimmer, C., Basyuk, E., & Bertrand, E.
- Venue
- Nature Communications
- Published
- 2016-07-27
- Volume
- 7
- Issue
- 1
- Pages
- 12248-12248
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2491614679
- Cited by
- 300
- References
- 63
- FWCI
- 12.04
- Type
- journal article
- Language
- en
- Open access
- gold
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- Tantale, K., Mueller, F., Kozulic-Pirher, A., Lesne, A., Victor, J.-M., Robert, M.-C., Capozi, S., Chouaib, R., Bäcker, V., Mateos-Langerak, J., Darzacq, X., Zimmer, C., Basyuk, E., & Bertrand, E. (2016). A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting. Nature Communications. https://doi.org/10.1038/ncomms12248
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