Cited by 112RNA and protein synthesis mechanismsMitochondrial Function and PathologyRNA Research and Splicing

Organization of mitochondrial gene expression in two distinct ribosome-containing assemblies

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Kehrein, Kirsten, Schilling, Ramon, Möller-Hergt, Braulio Vargas, Wurm, Christian A., Jakobs, Stefan, Lamkemeyer, Tobias, Langer, Thomas, Ott, Martin·2015·Cell Reports

Abstract

Mitochondria contain their own genetic system that provides subunits of the complexes driving oxidative phosphorylation. A quarter of the mitochondrial proteome participates in gene expression, but how all these factors are orchestrated and spatially organized is currently unknown. Here, we established a method to purify and analyze native and intact complexes of mitochondrial ribosomes. Quantitative mass spectrometry revealed extensive interactions of ribosomes with factors involved in all the steps of posttranscriptional gene expression. These interactions result in large expressosome-like assemblies that we termed mitochondrial organization of gene expression (MIOREX) complexes. Superresolution microscopy revealed that most MIOREX complexes are evenly distributed throughout the mitochondrial network, whereas a subset is present as nucleoid-MIOREX complexes that unite the whole spectrum of organellar gene expression. Our work therefore provides a conceptual framework for the spatial organization of mitochondrial protein synthesis that likely developed to facilitate gene expression in the organelle.

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Authors
Kehrein, K., Schilling, R., Möller-Hergt, B. V., Wurm, C. A., Jakobs, S., Lamkemeyer, T., Langer, T., & Ott, M.
Affiliations
  • Stockholm University (SE)
  • Max Planck Institute for Biophysical Chemistry (DE)
  • Universitätsmedizin Göttingen (DE)
  • University of Cologne (DE)
Corresponding author
Ott, Martin
Venue
Cell Reports
Publisher
Elsevier BV
Published
2015-02
Volume
10
Issue
6
Pages
843-853
ISSN
2211-1247 (ISSN-L)
OpenAlex
W2011474450
Cited by
112
References
52
Citations by year
2026: 4 · 2025: 6 · 2024: 11 · 2023: 12 · 2022: 8 · 2021: 14 · 2020: 15 · 2019: 14 · 2018: 9 · 2017: 7 · 2016: 7 · 2015: 5
FWCI
4.05
Type
journal article
Language
en
Open access
Locations
  • Cell Reports (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Kölner Universitäts PublikationsServer (Universität zu Köln) (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • GRO (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
Funders
  • University of Miami
  • University of Arizona
  • Knut och Alice Wallenbergs Stiftelse
  • Vetenskapsrådet
  • Stockholms Universitet
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
mitochondrial gene expression, mitochondrial ribosome, nucleoid, mitochondrial translation, oxidative phosphorylation, mitochondrial network

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