Organization of mitochondrial gene expression in two distinct ribosome-containing assemblies
Open accessAbstract
Mitochondria contain their own genetic system that provides subunits of the complexes driving oxidative phosphorylation. A quarter of the mitochondrial proteome participates in gene expression, but how all these factors are orchestrated and spatially organized is currently unknown. Here, we established a method to purify and analyze native and intact complexes of mitochondrial ribosomes. Quantitative mass spectrometry revealed extensive interactions of ribosomes with factors involved in all the steps of posttranscriptional gene expression. These interactions result in large expressosome-like assemblies that we termed mitochondrial organization of gene expression (MIOREX) complexes. Superresolution microscopy revealed that most MIOREX complexes are evenly distributed throughout the mitochondrial network, whereas a subset is present as nucleoid-MIOREX complexes that unite the whole spectrum of organellar gene expression. Our work therefore provides a conceptual framework for the spatial organization of mitochondrial protein synthesis that likely developed to facilitate gene expression in the organelle.
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CaPpr7 shows functional similarity to ScMrx1p, SpPpr10p, and Ncu08674 of N. crassa.
“Although CaPpr7 is not clearly orthologous to any known fungal PPR proteins, it shows some functional similarity to ScMrx1p (Kehrein et al. 2015), SpPpr10p (Wang et al. 2017), and Ncu08674 of N. crassa (Solotoff et al. 2015).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Kehrein et al. 2015
ScMrx1p is associated with ribosomes during translation and coupled to the mitochondrial membrane by the MIOREX complex.
“ScMrx1p is associated with ribosomes during translation, coupled to the mitochondrial membrane by the MIOREX complex (Kehrein et al. 2015).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Kehrein et al. 2015
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An Update on Mitochondrial Ribosome Biology: The Plant Mitoribosome in the Spotlight
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Regulation of mitochondrial translation of theATP8/ATP6mRNA by Smt1p
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Deficiency of mitoribosomal S10 protein affects translation and splicing in Arabidopsis mitochondria
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Activation of Yeast Mitochondrial Translation: Who Is in Charge?
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Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes
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Determining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC
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Mitochondrial protein phosphorylation in yeast revisited
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A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids
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Coordination of cytochrome bc1 complex assembly at MICOS
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The MIOREX complex - lean management of mitochondrial gene expression
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DPC29promotes post-initiation mitochondrial translation inSaccharomyces cerevisiae
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A molecular switch at the yeast mitoribosomal tunnel exit controls cytochrome b synthesis
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Self-citationHoshino, S., Kanemura, R., Kurita, D., Soutome, Y., Himeno, H., Takaine, M., Watanabe, M., & Nameki, N. (2021) · Communications Biology · doi:10.1038/s42003-021-01835-6 · Cited by 6 · see citing works
IRC3 Regulates Mitochondrial Translation in Response to Metabolic Cues in Saccharomyces cerevisiae
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Assembly of multicomponent machines in RNA metabolism: A common theme in mRNA decay pathways
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Early steps in the biogenesis of mitochondrially encoded oxidative phosphorylation subunits
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Salvatori, R., Aftab, W., Forné, I., Imhof, A., Ott, M., & Singh, A. P. (2020) · STAR Protocols · doi:10.1016/j.xpro.2020.100219 · Cited by 4 · see citing works
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Aim23p Interacts with the Yeast Mitochondrial Ribosomal Small Subunit
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Role of Rmd9p in 3′-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae
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Methods to Study the Biogenesis of Mitoribosomal Proteins in Yeast
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High resolution imaging of nascent mitochondrial protein synthesis in cultured human cellsPreprint
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Self-citationRapid Cryopurification of the Yeast Mitochondrial Ribosome
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Insights into the translational activation mechanisms of the COX1 mRNA in yeast mitochondria
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Mitochondrial DNA homeostasis: A novel therapeutic target for neurodegenerative diseases
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Assays to monitor mitochondrial translation
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Karadzas, M. T., Michel, A. H., Mosbach, A., Giannakopoulos, G., McGettigan, R., Scalliet, G., & Kornmann, B. (2025) · eLife · doi:10.7554/elife.105333.1 · Cited by 1 · see citing works
- Authors
- Kehrein, K., Schilling, R., Möller-Hergt, B. V., Wurm, C. A., Jakobs, S., Lamkemeyer, T., Langer, T., & Ott, M.
- Affiliations
- Stockholm University (SE)
- Max Planck Institute for Biophysical Chemistry (DE)
- Universitätsmedizin Göttingen (DE)
- University of Cologne (DE)
- Corresponding author
- Ott, Martin
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-02
- Volume
- 10
- Issue
- 6
- Pages
- 843-853
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2011474450
- Cited by
- 112
- References
- 52
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 11 · 2023: 12 · 2022: 8 · 2021: 14 · 2020: 15 · 2019: 14 · 2018: 9 · 2017: 7 · 2016: 7 · 2015: 5
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Kölner Universitäts PublikationsServer (Universität zu Köln) (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Funders
- University of Miami
- University of Arizona
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet
- Stockholms Universitet
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- mitochondrial gene expression, mitochondrial ribosome, nucleoid, mitochondrial translation, oxidative phosphorylation, mitochondrial network
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