The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes
Open accessAbstract
Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped potential of "coding introns" (i.e., introns between coding exons). Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest using intronically inserted "plug-and-play" cassettes (called "Trojan exons") that carry a splice acceptor site followed by the coding sequences of T2A peptide and an effector transgene. We demonstrate the efficacy of this approach in Drosophila using lines containing suitable MiMIC (Minos-mediated integration cassette) transposons and a palette of Trojan exons capable of expressing a range of commonly used transcription factors. We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system.
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Cites
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“Each Trojan exon is flanked by attB sites and a pair of uniquely compatible lox sites, which are substrates of the Cre recombinase (Livet et al., 2007).”
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A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex
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Refinement of Tools for Targeted Gene Expression in Drosophila
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
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Cited by
Liu, L., MacKenzie, K. R., Putluri, N., Maletić-Savatić, M., & Bellen, H. J. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.08.024 · Cited by 556
Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior
Nässel, D. R., & Zandawala, M. (2019) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2019.02.003 · Cited by 355
A genetic, genomic, and computational resource for exploring neural circuit function
Davis, F. P., Nern, A., Picard, S., Reiser, M. B., Rubin, G. M., Eddy, S. R., & Henry, G. L. (2020) · eLife · doi:10.7554/elife.50901 · Cited by 268
Neuronal diversity and convergence in a visual system developmental atlas
Özel, M. N., Simon, F., Jafari, S., Holguera, I., Chen, Y.-C., Benhra, N., El-Danaf, R. N., Kapuralin, K., Malin, J. A., Konstantinides, N., et al. (2021) · Nature · doi:10.1038/s41586-020-2879-3 · Cited by 237
Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing
Li, H., Horns, F., Wu, B., Xie, Q., Li, J., Li, T., Luginbuhl, D. J., Quake, S. R., & Luo, L. (2017) · Cell · doi:10.1016/j.cell.2017.10.019 · Cited by 265
Metadata
- Authors
- Diao, F., Ironfield, H., Luan, H., Diao, F., Shropshire, W. C., Ewer, J., Marr, E., Potter, C. J., Landgraf, M., & White, B. H.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 10
- Issue
- 8
- Pages
- 1410-1421
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2035505151
- Cited by
- 347
- References
- 49
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- gold
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- Diao, F., Ironfield, H., Luan, H., Diao, F., Shropshire, W. C., Ewer, J., Marr, E., Potter, C. J., Landgraf, M., & White, B. H. (2015). Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes. Cell Reports. https://doi.org/10.1016/j.celrep.2015.01.059
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