A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex
Open accessAbstract
A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized approximately 20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Transgenic rodents expressing fluorescent proteins or Cre/Flp recombinase under marker promoters allow identification and manipulation of specific interneuron groups.
“advances in molecular genetics have allowed the generation of transgenic rodents expressing fluorescent proteins or the enzymes Cre or Flp recombinase under the control of the promoters for these markers, allowing the identification and manipulation of specific groups of INs”
From GABAergic Interneurons in the Neocortex: From Cellular Properties to Circuits (2016) · cites this paper as Taniguchi et al., 2011
The Introduction and Results (Strategy and resource; page numbers unavailable) describe mouse Cre knockin lines targeted to endogenous genes, Cre/Flp-based targeting of interneuron populations, and fluorescent reporters used to identify them. The paper supports identification and manipulation, but its fluorescent reporters are at the Rosa26 locus and activated by recombination; it does not establish that those fluorescent proteins are expressed directly under the interneuron-marker promoters described in the claim. (LLM, conf=0.84)
Experimental — accuracy not yet measuredNot all CCK immunoreactive neurons are CCKBCs, as CCK can also be detected in glutamatergic principal cell subsets and additional subpopulations of dendrite targeting interneurons.
“However, not all CCK immunoreactive neurons are CCKBCs, as CCK can also be detected in glutamatergic principal cell subsets (1096, 494) and additional subpopulations of dendrite targeting interneurons (352, 1036).”
From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1096]
CCKBCs can be genetically targeted using CCK-Cre driver mice; however, additional CCK-expressing interneurons and even some glutamatergic principal cell subsets will also exhibit recombination.
“CCKBCs can be genetically targeted using CCK-Cre driver mice; however, additional CCK-expressing interneurons and even some glutamatergic principal cell subsets will also exhibit recombination (68, 1095, 1096).”
From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1096]
SST-expressing interneurons can be genetically accessed in developing and mature brains using SST-Cre driver lines.
“In general, SST-expressing interneurons can be genetically accessed in developing and mature brains using SST-Cre driver lines (702, 1096).”
From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1096]
Genetic access for manipulation of ISIs is available through use of VIP-Cre and CR-Cre driver lines of mice.
“Genetic access for manipulation of ISIs is available through use of VIP-Cre and CR-Cre driver lines of mice (1096).”
From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [1096]
Combined with cell type-restricted Cre driver lines, the R26-LSL-tAGO2 strategy allows systematic analysis of miRNA expression in different cell types in mice.
“Combined with an increasing inventory of cell type-restricted Cre driver lines (Taniguchi et al., 2011) this strategy would allow systematic analysis of miRNA expression in different cell types in mice.”
From Cell-Type-Based Analysis of MicroRNA Profiles in the Mouse Brain (2012) · cites this paper as Taniguchi et al., 2011
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 78 · Cited by 1,828
Research works printed but not linked: 1
- Cajal R La textura del sistema nerviosa del hombre y los vertebrados 1899 Moya Madrid 1st edition
- Authors
- Taniguchi, H., He, M., Wu, P., Kim, S., Paik, R., Sugino, K., Kvitsani, D., Fu, Y., Lu, J., Lin, Y., Miyoshi, G., Shima, Y., Fishell, G., Nelson, S. B., & Huang, Z. J.
- Affiliations
- Cold Spring Harbor Laboratory (US)
- Brandeis University (US)
- NYU Langone Health (US)
- Corresponding author
- Huang, Z. Josh
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2011-09
- Volume
- 71
- Issue
- 6
- Pages
- 995-1013
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC3779648
- OpenAlex
- W2040574649
- Cited by
- 1,828
- References
- 78
- Citations by year
- 2026: 77 · 2025: 105 · 2024: 146 · 2023: 156 · 2022: 152 · 2021: 156 · 2020: 177 · 2019: 157 · 2018: 129 · 2017: 124 · 2016: 111 · 2015: 112 · 2014: 104 · 2013: 72 · 2012: 42
- FWCI
- 29.16
- Type
- journal article
- Language
- en
- Update notices
- erratum (2011-12-22)
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Funders
- National Institute of Mental Health (U01MH078844)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- GABAergic interneurons, cerebral cortex, gene targeting, neural circuit assembly, cell fate specification, neuronal migration, interneuron connectivity, neural network dynamics, mouse genetic resources
- MeSH terms
- Animals, Cell Differentiation, Cell Line, Cerebral Cortex, gamma-Aminobutyric Acid, Gene Expression Regulation, Interneurons, Mice, Transgenic, Neurons, Stem Cells, Genes, Reporter, Integrases, Mice, Gene Knock-In Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
