The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons
Open accessAbstract
In the axons of cultured hippocampal neurons, actin forms various structures, including bundles, patches (involved in the preservation of neuronal polarity), and a recently reported periodic ring-like structure. Nevertheless, the overlaying organization of actin in neurons and in the axon initial segment (AIS) is still unclear, due mainly to a lack of adequate imaging methods. By harnessing live-cell stimulated emission depletion (STED) nanoscopy and the fluorescent probe SiR-Actin, we show that the periodic subcortical actin structure is in fact present in both axons and dendrites. The periodic cytoskeleton organization is also found in the peripheral nervous system, specifically at the nodes of Ranvier. The actin patches in the AIS co-localize with pre-synaptic markers. Cytosolic actin organization strongly depends on the developmental stage and subcellular localization. Altogether, the results of this study reveal unique neuronal cytoskeletal features.
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Cites
The establishment of polarity by hippocampal neurons in culture.
Dotti, C., Sullivan, C., & Banker, G. (1988) · The Journal of Neuroscience · doi:10.1523/jneurosci.08-04-01454.1988 · Cited by 1,862
The axon initial segment and the maintenance of neuronal polarity
Rasband, M. N. (2010) · Nature Reviews Neuroscience · doi:10.1038/nrn2852 · Cited by 442
Korobova, F., & Svitkina, T. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-07-0596 · Cited by 399
A selective filter for cytoplasmic transport at the axon initial segment
Song, A.-H., Wang, D., Chen, G., Li, Y., Luo, J., Duan, S., & Poo, M.-M. (2009) · Cell · doi:10.1016/j.cell.2009.01.016 · Cited by 322
Nanoscopy of living brain slices with low light levels
Testa, I., Urban, N. T., Jakobs, S., Eggeling, C., Willig, K. I., & Hell, S. W. (2012) · Neuron · doi:10.1016/j.neuron.2012.07.028 · Cited by 131
Self-citation
Cited by
Fluorescence nanoscopy in cell biology
Sahl, S. J., Hell, S. W., & Jakobs, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.71 · Cited by 990
Self-citationStimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Compartment-Specific Regulation of Autophagy in Primary Neurons
Maday, S., & Holzbaur, E. L. F. (2016) · The Journal of Neuroscience · doi:10.1523/jneurosci.4401-15.2016 · Cited by 309
Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings
Vassilopoulos, S., Gibaud, S., Jimenez, A., Caillol, G., & Leterrier, C. (2019) · Nature Communications · doi:10.1038/s41467-019-13835-6 · Cited by 143
An Integrated Cytoskeletal Model of Neurite Outgrowth
Miller, K. E., & Suter, D. M. (2018) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2018.00447 · Cited by 118
Metadata
- Authors
- D’Este, E., Kamin, D., Göttfert, F., El-Hady, A., & Hell, S. W.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 10
- Issue
- 8
- Pages
- 1246-1251
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2103705255
- Cited by
- 316
- References
- 5
- FWCI
- 17.04
- Type
- journal article
- Language
- en
- Open access
- gold
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- D’Este, E., Kamin, D., Göttfert, F., El-Hady, A., & Hell, S. W. (2015). STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons. Cell Reports. https://doi.org/10.1016/j.celrep.2015.02.007
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