Nanoscopy of living brain slices with low light levels
Open accessAbstract
Lens-based fluorescence microscopy, which has long been limited in resolution to about 200 nanometers by diffraction, is rapidly evolving into a nanoscale imaging technique. Here, we show that the superresolution fluorescence microscopy called RESOLFT enables comparatively fast and continuous imaging of sensitive, nanosized features in living brain tissue. Using low-intensity illumination to switch photochromic fluorescent proteins reversibly between a fluorescent and a nonfluorescent state, we increased the resolution more than 3-fold over that of confocal microscopy in all dimensions. Dendritic spines located 10-50 μm deep inside living organotypic hippocampal brain slices were recorded for hours without signs of degradation. Using a fast-switching protein increased the imaging speed 50-fold over reported RESOLFT schemes, which in turn enabled the recording of spontaneous and stimulated changes of dendritic actin filaments and spine morphology occurring on time scales from seconds to hours.
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RESOLFT requires orders of magnitude less light intensity and has been demonstrated on living brain slices.
“This approach requires orders of magnitude of less light intensity and has been demonstrated on living brain slices (Testa et al., 2012).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Testa et al., 2012
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Self-citation
- Authors
- Testa, I., Urban, N. T., Jakobs, S., Eggeling, C., Willig, K. I., & Hell, S. W.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- Max Planck Society (DE)
- Corresponding author
- Hell, Stefan W.
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 75
- Issue
- 6
- Pages
- 992-1000
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2054093040
- Cited by
- 131
- References
- 49
- Citations by year
- 2026: 4 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 13 · 2020: 7 · 2019: 7 · 2018: 11 · 2017: 8 · 2016: 12 · 2015: 15 · 2014: 19 · 2013: 21 · 2012: 2
- FWCI
- 8.34
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- OPUS (Augsburg University) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Physical Sciences › Materials Science › Materials Chemistry › Photochromic and Fluorescence Chemistry
- Keywords
- RESOLFT, super-resolution fluorescence microscopy, dendritic spines, organotypic hippocampal slices, low-light conditions, diffraction-limited resolution
- MeSH terms
- Animals, Animals, Newborn, Electric Stimulation, Hippocampus, Image Processing, Computer-Assisted, Light, Luminescent Proteins, Mice, Inbred C57BL, Neurons, Organ Culture Techniques, Time Factors, Transfection, Long-Term Potentiation, Patch-Clamp Techniques, Microscopy, Confocal, Nanotechnology, Dendritic Spines, Mice
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