CoChat
Sign Up
Cited by 21

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA

Banks, Kelly M., Lan, Yahui, Evans, Todd·2021·Cell Reports
DOI 10.1016/j.celrep.2020.108632PMC PMC7837371
Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.celrep.2020.108632

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1. Granulation Is Defective in tet3−/− and tet2/3DM Embryonic Neutrophils Representative images of neutrophil staining in wild-type, tet2−/−, tet3−/−, and tet2/3DM embryos at 3 dpf. (A–D) Caudal hematopoietic tissue (CHT) region following WISH to analyze mpx transcripts. (E–H) CHT region of embryos stained for Sudan black (SB). Scale bars in (A)–(H) represent 200 μm. (I–L) High-resolution images of SB-stained neutrophils. Scale bars represent 2 μm. (M and N) Quantification of total mpx+ (immature and mature neutrophils) and SB+ (mature neutrophils) cells in embryos at 2 dpf (M) and 3 dpf (N); n = 10/condition, *p < 0.05, ***p < 0.001, ****p < 0.0001 by 2-way ANOVA with Tukey post hoc test. See also Figure S1.
Figure 1. Granulation Is Defective in tet3−/− and tet2/3DM Embryonic Neutrophils Representative images of neutrophil staining in wild-type, tet2−/−, tet3−/−, and tet2/3DM embryos at 3 dpf. (A–D) Caudal hematopoietic tissue (CHT) region following WISH to analyze mpx transcripts. (E–H) CHT region of embryos stained for Sudan black (SB). Scale bars in (A)–(H) represent 200 μm. (I–L) High-resolution images of SB-stained neutrophils. Scale bars represent 2 μm. (M and N) Quantification of total mpx+ (immature and mature neutrophils) and SB+ (mature neutrophils) cells in embryos at 2 dpf (M) and 3 dpf (N); n = 10/condition, *p < 0.05, ***p < 0.001, ****p < 0.0001 by 2-way ANOVA with Tukey post hoc test. See also Figure S1.CC BY-NC-ND · PubMed Central
Figure 2. Human TET3 Overexpression Rescues the Granulation Defect in tet2/3DM Embryos (A) Quantification of SB+ cells in the CHT region for uninjected, 30 pg wild-type hTET3 and 30 pg catalytically dead mutant (HxD) hTET3-injected wild-type and tet2/3DM embryos at 3 dpf; n = 6/condition, *p < 0.05, ***p < 0.001, ****p < 0.0001 by 2-way ANOVA with Tukey post hoc test. (B–G) Representative images of the CHT of SB-stained uninjected, wild-type hTET3-injected, or mutant hTET3-injected wild-type and tet2/3DM embryos at 3 dpf. Scale bars represent 200 μm. See also Figure S2.
Figure 2. Human TET3 Overexpression Rescues the Granulation Defect in tet2/3DM Embryos (A) Quantification of SB+ cells in the CHT region for uninjected, 30 pg wild-type hTET3 and 30 pg catalytically dead mutant (HxD) hTET3-injected wild-type and tet2/3DM embryos at 3 dpf; n = 6/condition, *p < 0.05, ***p < 0.001, ****p < 0.0001 by 2-way ANOVA with Tukey post hoc test. (B–G) Representative images of the CHT of SB-stained uninjected, wild-type hTET3-injected, or mutant hTET3-injected wild-type and tet2/3DM embryos at 3 dpf. Scale bars represent 200 μm. See also Figure S2.CC BY-NC-ND · PubMed Central
Figure 3. Phagocytosis Defect Is Unique to tet2/3DM Embryonic Neutrophils (A–D) Representative images of the CHT region in pHrodo-red-injected 3-dpf wild-type, tet2−/−/, tet3−/−, and tet2/3DM mpx:GFP embryos. Scale bars represent 200 μm. (E) Quantification of phagocytosed beads by fluorescent area within GFP+ neutrophils in pixels squared. (F) Quantification of lysosome acidification by mean fluorescence intensity in arbitrary fluorescence units (A.F.U.). n = 5–7 embryos/condition, **p < 0.01, ****p < 0.0001 by 2-way ANOVA with Tukey post hoc test of each condition compared to wild type. See also Figure S3.
Figure 3. Phagocytosis Defect Is Unique to tet2/3DM Embryonic Neutrophils (A–D) Representative images of the CHT region in pHrodo-red-injected 3-dpf wild-type, tet2−/−/, tet3−/−, and tet2/3DM mpx:GFP embryos. Scale bars represent 200 μm. (E) Quantification of phagocytosed beads by fluorescent area within GFP+ neutrophils in pixels squared. (F) Quantification of lysosome acidification by mean fluorescence intensity in arbitrary fluorescence units (A.F.U.). n = 5–7 embryos/condition, **p < 0.01, ****p < 0.0001 by 2-way ANOVA with Tukey post hoc test of each condition compared to wild type. See also Figure S3.CC BY-NC-ND · PubMed Central
Figure 4. Granulation Defects Are Also Found in tet2/3MH Adult Neutrophils (A–D) Representative flow cytometry analysis of whole-kidney marrow (WKM) for 1-year-old wild-type, tet2−/−, tet3−/−, and tet2/3MH zebrafish. Myeloid cells are delineated by the black circle, hematopoietic progenitors by a dark gray circle, lymphocytes by a medium gray circle, and red blood cells (RBCs) by a light gray circle. (E–H) Representative electron microscopy images of wild-type, tet2−/−, tet3−/−, and tet2/3MH neutrophils isolated by FACS from kidneys of 1-year-old adult zebrafish. Red arrowheads indicate representative granules, blue arrowheads indicate autophagosomes, and the green arrowhead indicates nuclear envelope separation. (I) Quantification of the percentage of cells of each subtype: myeloid cells, progenitors, lymphocytes, and RBCs; n = 3/condition. (J) Histogram comparing FSC-A for the myeloid gate in each genotype for a representative sample. (K) Quantification of mean FSC-A for the myeloid gate in each genotype; n = 3/condition. (L) Quantification of granules per cell in electron microscopy images; n = 4/condition. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to wild type by one-way ANOVA with Tukey post hoc test.
Figure 4. Granulation Defects Are Also Found in tet2/3MH Adult Neutrophils (A–D) Representative flow cytometry analysis of whole-kidney marrow (WKM) for 1-year-old wild-type, tet2−/−, tet3−/−, and tet2/3MH zebrafish. Myeloid cells are delineated by the black circle, hematopoietic progenitors by a dark gray circle, lymphocytes by a medium gray circle, and red blood cells (RBCs) by a light gray circle. (E–H) Representative electron microscopy images of wild-type, tet2−/−, tet3−/−, and tet2/3MH neutrophils isolated by FACS from kidneys of 1-year-old adult zebrafish. Red arrowheads indicate representative granules, blue arrowheads indicate autophagosomes, and the green arrowhead indicates nuclear envelope separation. (I) Quantification of the percentage of cells of each subtype: myeloid cells, progenitors, lymphocytes, and RBCs; n = 3/condition. (J) Histogram comparing FSC-A for the myeloid gate in each genotype for a representative sample. (K) Quantification of mean FSC-A for the myeloid gate in each genotype; n = 3/condition. (L) Quantification of granules per cell in electron microscopy images; n = 4/condition. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to wild type by one-way ANOVA with Tukey post hoc test.CC BY-NC-ND · PubMed Central
Figure 5. Transcriptional Profiling of tet Mutant Neutrophils Reveals Reduced Cytokine Response in tet3−/− and tet2/3DM Analysis of RNA sequencing from sorted neutrophils of 35-hpf wild-type, 2-dpf wild-type, 2-dpf tet2−/−, 2-dpf tet3−/−, and 2-dpf tet2/3DM embryos. (A) Principal component analysis for all samples, with three independent biological samples representing each genotype, as indicated by colored circles. (B) Enriched GO terms related to hematopoiesis in genes downregulated in 2-dpf tet2/3DM samples compared to 2-dpf wild-type samples. (C) Volcano plot comparing 2-dpf tet3−/− and wild-type samples; the top 20 dysregulated genes are labeled. (D) Top 10 hallmark GSEA terms downregulated comparing 2-dpf tet3−/− and wild-type samples; categories involved in cytokine response are highlighted in red. (E) Volcano plot comparing 2-dpf tet2/3DM and wild-type samples; the top 20 dysregulated genes are labeled. (F) Top 10 hallmark GSEA terms downregulated comparing 2-dpf tet2/3DM and wild-type samples; categories involved in cytokine response are highlighted in red. See also Figures S4 and S6 and Table S1.
Figure 5. Transcriptional Profiling of tet Mutant Neutrophils Reveals Reduced Cytokine Response in tet3−/− and tet2/3DM Analysis of RNA sequencing from sorted neutrophils of 35-hpf wild-type, 2-dpf wild-type, 2-dpf tet2−/−, 2-dpf tet3−/−, and 2-dpf tet2/3DM embryos. (A) Principal component analysis for all samples, with three independent biological samples representing each genotype, as indicated by colored circles. (B) Enriched GO terms related to hematopoiesis in genes downregulated in 2-dpf tet2/3DM samples compared to 2-dpf wild-type samples. (C) Volcano plot comparing 2-dpf tet3−/− and wild-type samples; the top 20 dysregulated genes are labeled. (D) Top 10 hallmark GSEA terms downregulated comparing 2-dpf tet3−/− and wild-type samples; categories involved in cytokine response are highlighted in red. (E) Volcano plot comparing 2-dpf tet2/3DM and wild-type samples; the top 20 dysregulated genes are labeled. (F) Top 10 hallmark GSEA terms downregulated comparing 2-dpf tet2/3DM and wild-type samples; categories involved in cytokine response are highlighted in red. See also Figures S4 and S6 and Table S1.CC BY-NC-ND · PubMed Central
Figure 6. Socs3b mRNA Levels Are Increased in tet Mutant Neutrophils and Mutation of socs3b Rescues the Neutrophil Granulation Defect in tet2/3DM Embryos (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of socs3b gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3DM embryos. Scale bars represent 500 μm. See also Figures S4 and S5.
Figure 6. Socs3b mRNA Levels Are Increased in tet Mutant Neutrophils and Mutation of socs3b Rescues the Neutrophil Granulation Defect in tet2/3DM Embryos (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of socs3b gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3DM embryos. Scale bars represent 500 μm. See also Figures S4 and S5.CC BY-NC-ND · PubMed Central
Figure 7. Socs3b mRNA Is Hypermethylated in tet2/3DM Neutrophils (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, *p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b. DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure. See also Figure S6 and Data S1.
Figure 7. Socs3b mRNA Is Hypermethylated in tet2/3DM Neutrophils (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, *p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b. DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure. See also Figure S6 and Data S1.CC BY-NC-ND · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 5 references

Research works printed but not linked: 52

  1. Adams, 1975, Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNA, Nature, 255, 28, 10.1038/255028a0 · DOI
  2. Amort, 2017, Detection of 5-methylcytosine in specific poly(A) RNAs by bisulfite sequencing, Methods Mol. Biol., 1562, 107, 10.1007/978-1-4939-6807-7_8 · DOI
  3. Anelli, 2017, Oncogenic BRAF disrupts thyroid morphogenesis and function via twist expression, eLife, 6, e20728, 10.7554/eLife.20728 · DOI
  4. Bock, 2012, DNA methylation dynamics during in vivo differentiation of blood and skin stem cells, Mol. Cell, 47, 633, 10.1016/j.molcel.2012.06.019 · DOI
  5. Chakraborty, 1998, Stat3 and G-CSF-induced myeloid differentiation, Leuk. Lymphoma, 30, 433, 10.3109/10428199809057555 · DOI
  6. Chatterjee, 2015, Genome-wide DNA methylation map of human neutrophils reveals widespread inter-individual epigenetic variation, Sci. Rep., 5, 17328, 10.1038/srep17328 · DOI
  7. Dancey, 1976, Neutrophil kinetics in man, J. Clin. Invest., 58, 705, 10.1172/JCI108517 · DOI
  8. Deaton, 2011, Cell type-specific DNA methylation at intragenic CpG islands in the immune system, Genome Res., 21, 1074, 10.1101/gr.118703.110 · DOI
  9. de Jong, 2005, Use of the zebrafish system to study primitive and definitive hematopoiesis, Annu. Rev. Genet., 39, 481, 10.1146/annurev.genet.39.073003.095931 · DOI
  10. Delatte, 2016, RNA biochemistry. Transcriptome-wide distribution and function of RNA hydroxymethylcytosine, Science, 351, 282, 10.1126/science.aac5253 · DOI · Paper page
  11. Dobson, 2008, Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination, Blood, 112, 2969, 10.1182/blood-2008-03-145011 · DOI
  12. Döring, 2015, Neutrophils in atherosclerosis: from mice to man, Arterioscler. Thromb. Vasc. Biol., 35, 288, 10.1161/ATVBAHA.114.303564 · DOI
  13. Dubin, 1975, The methylation state of poly A-containing messenger RNA from cultured hamster cells, Nucleic Acids Res., 2, 1653, 10.1093/nar/2.10.1653 · DOI
  14. Fan, 2014, miR-142-3p acts as an essential modulator of neutrophil development in zebrafish, Blood, 124, 1320, 10.1182/blood-2013-12-545012 · DOI
  15. Fu, 2014, Tet-mediated formation of 5-hydroxymethylcytosine in RNA, J. Am. Chem. Soc., 136, 11582, 10.1021/ja505305z · DOI · Paper page
  16. Gerlach, 2011, Dissection of the adult zebrafish kidney, J. Vis. Exp., 2839
  17. Gombart, 2005, Aberrant expression of neutrophil and macrophage-related genes in a murine model for human neutrophil-specific granule deficiency, J. Leukoc. Biol., 78, 1153, 10.1189/jlb.0504286 · DOI
  18. Grassi, 2018, Dynamics of transcription regulation in human bone marrow myeloid differentiation to mature blood neutrophils, Cell Rep., 24, 2784, 10.1016/j.celrep.2018.08.018 · DOI
  19. Gruber, 2008, The Vienna RNA websuite, Nucleic Acids Res., 36, W70, 10.1093/nar/gkn188 · DOI
  20. Heinzelmann, 1999, Neutrophils and renal failure, Am. J. Kidney Dis., 34, 384, 10.1016/S0272-6386(99)70375-6 · DOI
  21. Hsu, 2004, The pu.1 promoter drives myeloid gene expression in zebrafish, Blood, 104, 1291, 10.1182/blood-2003-09-3105 · DOI
  22. Hsu, 2019, Immature low-density neutrophils exhibit metabolic flexibility that facilitates breast cancer liver metastasis, Cell Rep., 27, 3902, 10.1016/j.celrep.2019.05.091 · DOI
  23. Huber, 2015, Formation and abundance of 5-hydroxymethylcytosine in RNA, ChemBioChem, 16, 752, 10.1002/cbic.201500013 · DOI
  24. Hussain, 2013, NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs, Cell Rep., 4, 255, 10.1016/j.celrep.2013.06.029 · DOI
  25. Ichiyama, 2015, The methylcytosine dioxygenase Tet2 promotes DNA demethylation and activation of cytokine gene expression in T cells, Immunity, 42, 613, 10.1016/j.immuni.2015.03.005 · DOI · Paper page
  26. Jowett, 1999, Analysis of protein and gene expression, Methods Cell Biol., 59, 63, 10.1016/S0091-679X(08)61821-X · DOI
  27. Kaplan, 2012, Neutrophil extracellular traps: double-edged swords of innate immunity, J. Immunol., 189, 2689, 10.4049/jimmunol.1201719 · DOI
  28. Ko, 2010, Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2, Nature, 468, 839, 10.1038/nature09586 · DOI · Paper page
  29. Lan, 2019, TETs regulate proepicardial cell migration through extracellular matrix organization during zebrafish cardiogenesis, Cell Rep., 26, 720, 10.1016/j.celrep.2018.12.076 · DOI
  30. Langlois, 2014, TET2 deficiency inhibits mesoderm and hematopoietic differentiation in human embryonic stem cells, Stem Cells, 32, 2084, 10.1002/stem.1718 · DOI
  31. Langmead, 2012, Fast gapped-read alignment with Bowtie 2, Nat. Methods, 9, 357, 10.1038/nmeth.1923 · DOI · Paper page
  32. Le Guyader, 2008, Origins and unconventional behavior of neutrophils in developing zebrafish, Blood, 111, 132, 10.1182/blood-2007-06-095398 · DOI
  33. Li, 2015, Overlapping requirements for Tet2 and Tet3 in normal development and hematopoietic stem cell emergence, Cell Rep., 12, 1133, 10.1016/j.celrep.2015.07.025 · DOI
  34. Lieschke, 2002, Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning, Dev. Biol., 246, 274, 10.1006/dbio.2002.0657 · DOI · Paper page
  35. McCurley, 2008, Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment, BMC Mol. Biol., 9, 102, 10.1186/1471-2199-9-102 · DOI
  36. Montagner, 2016, TET2 regulates mast cell differentiation and proliferation through catalytic and non-catalytic activities, Cell Rep., 15, 1566, 10.1016/j.celrep.2016.04.044 · DOI · Paper page
  37. Paz, 2014, RBPmap: a web server for mapping binding sites of RNA-binding proteins, Nucleic Acids Res., 42, W361, 10.1093/nar/gku406 · DOI
  38. Rampal, 2014, DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemia, Cell Rep., 9, 1841, 10.1016/j.celrep.2014.11.004 · DOI · Paper page
  39. Rönnerblad, 2014, Analysis of the DNA methylome and transcriptome in granulopoiesis reveals timed changes and dynamic enhancer methylation, Blood, 123, e79, 10.1182/blood-2013-02-482893 · DOI
  40. Saletore, 2012, The birth of the epitranscriptome: deciphering the function of RNA modifications, Genome Biol., 13, 175, 10.1186/gb-2012-13-10-175 · DOI
  41. Schneider, 2012, NIH Image to ImageJ: 25 years of image analysis, Nat. Methods, 9, 671, 10.1038/nmeth.2089 · DOI · Paper page
  42. Shen, 2018, Tet2 promotes pathogen infection-induced myelopoiesis through mRNA oxidation, Nature, 554, 123, 10.1038/nature25434 · DOI · Paper page
  43. Skokowa, 2017, Severe congenital neutropenias, Nat. Rev. Dis. Primers, 3, 17032, 10.1038/nrdp.2017.32 · DOI
  44. Squires, 2012, Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA, Nucleic Acids Res., 40, 5023, 10.1093/nar/gks144 · DOI
  45. Stachura, 2009, Zebrafish kidney stromal cell lines support multilineage hematopoiesis, Blood, 114, 279, 10.1182/blood-2009-02-203638 · DOI
  46. Trengove, 2013, SOCS proteins in development and disease, Am. J. Clin. Exp. Immunol., 2, 1
  47. Tsagaratou, 2017, TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells, Nat. Immunol., 18, 45, 10.1038/ni.3630 · DOI · Paper page
  48. Verma, 2018, TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells, Nat. Genet., 50, 83, 10.1038/s41588-017-0002-y · DOI
  49. Yang, 2017, 5-methylcytosine promotes mRNA export—NSUN2 as the methyltransferase and ALYREF as an m5C reader, Cell Res., 27, 606, 10.1038/cr.2017.55 · DOI · Paper page
  50. Yang, 2019, RNA 5-methylcytosine facilitates the maternal-to-zygotic transition by preventing maternal mRNA decay, Mol. Cell, 75, 1188, 10.1016/j.molcel.2019.06.033 · DOI
  51. Yoo, 2011, Spatiotemporal photolabeling of neutrophil trafficking during inflammation in live zebrafish, J. Leukoc. Biol., 89, 661, 10.1189/jlb.1010567 · DOI
  52. Zhao, 2017, Post-transcriptional gene regulation by mRNA modifications, Nat. Rev. Mol. Cell Biol., 18, 31, 10.1038/nrm.2016.132 · DOI

Cited by

See all 21

Metadata

Authors
Banks, K. M., Lan, Y., & Evans, T.
Venue
Cell Reports
Publisher
Elsevier BV
Published
2021-01
Volume
34
Issue
2
Pages
108632
ISSN
2211-1247
DOI
10.1016/j.celrep.2020.108632
PMC
PMC7837371
Cited by
21
References
5
Language
en
Licence
Funders
  • New York State Department of Health
  • National Institutes of Health

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA. https://app.cochat.ai/papers/10.1016/j.celrep.2020.108632
The paper
Banks, K. M., Lan, Y., & Evans, T. (2021). Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA. Cell Reports. https://doi.org/10.1016/j.celrep.2020.108632