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Cited by 9RNA and protein synthesis mechanismsHeat shock proteins researchRNA Research and Splicing

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Heat shock induces silent ribosomes and reorganizes mRNA turnover

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Rahaman, Sayanur, Schiffelholz, Nicole, Mittal, Nitish, Fröhlich, Klemens E., Zavolan, Mihaela, Becskei, Attila·2025·Cell Reports
DOI 10.1016/j.celrep.2025.116447

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Abstract

mRNAs associate with single or multiple ribosomes; these ribosomal assemblies-monosomes and polysomes-translate the mRNAs before degradation. The impact of heat stress on this mRNA turnover remains unclear. We show that in heat-shocked yeast cells, the proportion of monosomes increases without a corresponding rise in the number of associated mRNAs. Consequently, most monosomes are devoid of mRNAs and silent, lacking translational initiation factors and proteins facilitating posttranslational folding. Such silent monosomes also appear under other stress conditions, with proportions varying according to stress type, suggesting that they represent a general feature of cellular adaptation. In parallel with the induction of silent ribosomes, elevated temperatures reduce the overall rate of mRNA-ribosome association with few exceptions. Notably, heat shock promotes the ribosomal association of transcripts encoding heat shock proteins, without extension of the half-lives of these mRNAs. These mechanisms dynamically reorganize mRNA turnover to prioritize the translation of heat shock proteins over other proteins.

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Metadata

Authors
Rahaman, S., Schiffelholz, N., Mittal, N., Fröhlich, K. E., Zavolan, M., & Becskei, A.
Affiliations
University of Basel (CH)
Corresponding author
Becskei, Attila
ORCID
Venue
Cell Reports
Publisher
Elsevier BV
Published
2025-10
Volume
44
Issue
10
Pages
116447
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2025.116447
OpenAlex
W4415305008
Cited by
9
References
1
Citations by year
2026: 6 · 2025: 3
FWCI
2.72
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Reports (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
Swiss National Science Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
heat shock, monosomes, polysomes, mRNA turnover, heat shock proteins, translational reprogramming, stress adaptation, yeast
MeSH terms
Heat-Shock Proteins, Polyribosomes, Ribosomes, RNA, Messenger, Saccharomyces cerevisiae, Protein Biosynthesis, Heat-Shock Response, RNA Stability, Saccharomyces cerevisiae Proteins

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