Cited by 231Microtubule and mitosis dynamicsCancer Treatment and PharmacologyHeterocyclic Compound Synthesis and Bioactivity

The DOI matches its Crossref record; 46 of the 47 references it cites were found in a registry or an index.

Microtubule Interactions with Chemically Diverse Stabilizing Agents: Thermodynamics of Binding to the Paclitaxel Site Predicts Cytotoxicity

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Abstract

The interactions of microtubules with most compounds described as stabilizing agents have been studied. Several of them (lonafarnib, dicumarol, lutein, and jatrophane polyesters) did not show any stabilizing effect on microtubules. Taccalonolides A and E show paclitaxel-like effects in cells, but they were not able to modulate in vitro tubulin assembly or to bind microtubules, which suggests that other factors are involved in their cellular effects. The binding constants of epothilones, eleutherobin, discodermolide, sarcodictyins, 3,17beta-diacetoxy-2-ethoxy-6-oxo-B-homo-estra-1,3,5(10)-triene, and dictyostatin to the paclitaxel site; the critical concentrations of ligand-induced assembly; and their cytotoxicity in carcinoma cells have been measured, and correlations between these parameters have been determined. The inhibition of cell proliferation correlates better with the binding enthalpy change than with the binding constants, suggesting that large, favorable enthalpic contribution to the binding is desired to design paclitaxel site drugs with higher cytotoxicity.

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Crossref · 10.1016/j.chembiol.2005.09.010

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Metadata

Authors
Buey, R. M., Barasoain, I., Jackson, E., Meyer, A., Giannakakou, P., Paterson, I., Mooberry, S., Andreu, J. M., & Díaz, J. F.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Texas Biomedical Research Institute (US)
  • University of Cambridge (GB)
  • Emory University (US)
Corresponding author
Díaz, J. Fernando
Venue
Chemistry & Biology
Publisher
Elsevier BV
Published
2005-12
Volume
12
Issue
12
Pages
1269-1279
ISSN
1074-5521 (ISSN-L)
OpenAlex
W2116826087
Cited by
231
References
47
Citations by year
2026: 7 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 15 · 2021: 8 · 2020: 11 · 2019: 7 · 2018: 3 · 2017: 13 · 2016: 8 · 2015: 13 · 2014: 15 · 2013: 18 · 2012: 7
FWCI
5.30
Type
journal article
Language
en
Open access
Locations
  • Chemistry & Biology (publishedVersion)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
  • Physical Sciences › Chemistry › Organic Chemistry › Heterocyclic Compound Synthesis and Bioactivity
Keywords
microtubule-stabilizing agents, binding enthalpy, cytotoxicity, cell proliferation inhibition, epothilones, lonafarnib, dicumarol, lutein, tubulin assembly, binding constant, drug design
MeSH terms
Antineoplastic Agents, Binding, Competitive, Cell Cycle, Humans, Ligands, Microtubules, Predictive Value of Tests, Protein Binding, Thermodynamics, Tubulin, Tumor Cells, Cultured, Drug Design, Molecular Structure, Paclitaxel, Tubulin Modulators

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