The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Expanding the Genetic Code for Biological Studies
Open accessAbstract
Summary Using an orthogonal tRNA-synthetase pair, unnatural amino acids can be genetically encoded with high efficiency and fidelity; and over forty unnatural amino acids have been site-specifically incorporated into proteins in E. coli, yeast, or mammalian cells. Novel chemical or physical properties embodied in these amino acids enable new means for tailored manipulation of proteins. This review summarizes the methodology and recent progress in expanding this technology to eukaryotic cells. Applications of genetically encoded unnatural amino acids are highlighted with reports on labeling and modifying proteins, probing protein structure and function, identifying and regulating protein activity, and generating proteins with new properties. Genetic incorporation of unnatural amino acids provides a powerful method for investigating a wide variety of biological processes both in vitro and in vivo.
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Cites
A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins
Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989) · Science · doi:10.1126/science.2649980 · Cited by 1,019 · cited 1×
“When added into cell extracts or Xenopus oocytes that support protein translation, the acylated tRNA incorporates the attached unnatural amino acid in response to a stop or extended codon (Noren et al., 1989; Bain et al., 1989; Nowak et al., 1995).”
BackgroundChemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation
McGinty, R. K., Kim, J., Chatterjee, C., Roeder, R. G., & Muir, T. W. (2008) · Nature · doi:10.1038/nature06906 · Cited by 563
Rewiring the keyboard: evolvability of the genetic code
Knight, R. D., Freeland, S. J., & Landweber, L. F. (2001) · Nature Reviews Genetics · doi:10.1038/35047500 · Cited by 435
Non-canonical amino acids in protein engineering
Link, A. J., Mock, M. L., & Tirrell, D. A. (2003) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2003.10.011 · Cited by 393
Natural expansion of the genetic code
Ambrogelly, A., Palioura, S., & Söll, D. (2007) · Nature Chemical Biology · doi:10.1038/nchembio847 · Cited by 361
Biosynthetic site-specific incorporation of a non-natural amino acid into a polypeptide
Bain, J. D., Diala, E. S., Glabe, C. G., Dix, T. A., & Chamberlin, A. R. (1989) · Journal of the American Chemical Society · doi:10.1021/ja00202a052 · Cited by 250
Cited by
Modulators of Protein–Protein Interactions
Milroy, L.-G., Grossmann, T. N., Hennig, S., Brunsveld, L., & Ottmann, C. (2014) · Chemical Reviews · doi:10.1021/cr400698c · Cited by 497
Currin, A., Swainston, N., Day, P. J., & Kell, D. B. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00351a · Cited by 388
Next-generation synthetic gene networks
Lu, T. K., Khalil, A. S., & Collins, J. J. (2009) · Nature Biotechnology · doi:10.1038/nbt.1591 · Cited by 365
Systematic Mapping of Genetic Interaction Networks
Dixon, S. J., Costanzo, M., Baryshnikova, A., Andrews, B., & Boone, C. (2009) · Annual Review of Genetics · doi:10.1146/annurev.genet.39.073003.114751 · Cited by 295
Advances in ultrahigh-throughput screening for directed enzyme evolution
Markel, U., Essani, K. D., Besirlioglu, V., Schiffels, J., Streit, W. R., & Schwaneberg, U. (2020) · Chemical Society Reviews · doi:10.1039/c8cs00981c · Cited by 258
Metadata
- Authors
- Wang, Q., Parrish, A. R., & Wang, L.
- Venue
- Chemistry & Biology
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 16
- Issue
- 3
- Pages
- 323-336
- ISSN
- 1074-5521 (ISSN-L)
- OpenAlex
- W2146913917
- Cited by
- 224
- References
- 6
- FWCI
- 7.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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