Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 383

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Coronaviruses Hijack the LC3-I-Positive EDEMosomes, ER-Derived Vesicles Exporting Short-Lived ERAD Regulators, for Replication

Open access
Reggiori, Fulvio, Monastyrska, Iryna, Verheije, Monique H., Calì, Tito, Ulasli, Mustafa, Bianchi, Siro, Bernasconi, Riccardo, de Haan, Cornelis A.M., Molinari, Maurizio·2010·Cell Host & Microbe
DOI 10.1016/j.chom.2010.05.013
Open this paper in CoChat — free account

Abstract

Coronaviruses (CoV), including SARS and mouse hepatitis virus (MHV), are enveloped RNA viruses that induce formation of double-membrane vesicles (DMVs) and target their replication and transcription complexes (RTCs) on the DMV-limiting membranes. The DMV biogenesis has been connected with the early secretory pathway. CoV-induced DMVs, however, lack conventional endoplasmic reticulum (ER) or Golgi protein markers, leaving their membrane origins in question. We show that MHV co-opts the host cell machinery for COPII-independent vesicular ER export of a short-living regulator of ER-associated degradation (ERAD), EDEM1, to derive cellular membranes for replication. MHV infection causes accumulation of EDEM1 and OS-9, another short-living ER chaperone, in the DMVs. DMVs are coated with the nonlipidated LC3/Atg8 autophagy marker. Downregulation of LC3, but not inactivation of host cell autophagy, protects cells from CoV infection. Our study identifies the host cellular pathway hijacked for supplying CoV replication membranes and describes an autophagy-independent role for nonlipidated LC3-I.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.chom.2010.05.013

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 3 references

Research works printed but not linked: 36

  1. Bernasconi, 2008, A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal, J. Biol. Chem., 283, 16446, 10.1074/jbc.M802272200 · DOI
  2. Bjørkøy, 2009, Monitoring autophagic degradation of p62/SQSTM1, Methods Enzymol., 452, 181, 10.1016/S0076-6879(08)03612-4 · DOI · Paper page
  3. Calì, 2008, Segregation and rapid turnover of EDEM1 by an autophagy-like mechanism modulates standard ERAD and folding activities, Biochem. Biophys. Res. Commun., 371, 405, 10.1016/j.bbrc.2008.04.098 · DOI
  4. Calì, 2008, The endoplasmic reticulum crossroads for newly synthesized polypeptide chains, Prog. Mol. Biol. Transl. Sci., 83, 135, 10.1016/S0079-6603(08)00604-1 · DOI
  5. Christianson, 2008, OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD, Nat. Cell Biol., 10, 272, 10.1038/ncb1689 · DOI · Paper page
  6. de Haan, 2008, Are nidoviruses hijacking the autophagy machinery?, Autophagy, 4, 276, 10.4161/auto.5241 · DOI
  7. Gosert, 2002, RNA replication of mouse hepatitis virus takes place at double-membrane vesicles, J. Virol., 76, 3697, 10.1128/JVI.76.8.3697-3708.2002 · DOI · Paper page
  8. Harcourt, 2004, Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity, J. Virol., 78, 13600, 10.1128/JVI.78.24.13600-13612.2004 · DOI · Paper page
  9. Hebert, 2007, In and out of the ER: protein folding, quality control, degradation, and related human diseases, Physiol. Rev., 87, 1377, 10.1152/physrev.00050.2006 · DOI · Paper page
  10. Kanjanahaluethai, 2007, Membrane topology of murine coronavirus replicase nonstructural protein 3, Virology, 361, 391, 10.1016/j.virol.2006.12.009 · DOI
  11. Knoops, 2008, SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum, PLoS Biol., 6, e226, 10.1371/journal.pbio.0060226 · DOI · Paper page
  12. Knoops, 2010, Integrity of the early secretory pathway promotes, but is not required for, severe acute respiratory syndrome coronavirus RNA synthesis and virus-induced remodeling of endoplasmic reticulum membranes, J. Virol., 84, 833, 10.1128/JVI.01826-09 · DOI
  13. Le Fourn, 2009, Basal autophagy is involved in the degradation of the ERAD component EDEM1, Cell. Mol. Life Sci., 66, 1434, 10.1007/s00018-009-9038-1 · DOI
  14. Miller, 2008, Modification of intracellular membrane structures for virus replication, Nat. Rev. Microbiol., 6, 363, 10.1038/nrmicro1890 · DOI · Paper page
  15. Mizushima, 2001, Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells, J. Cell Biol., 152, 657, 10.1083/jcb.152.4.657 · DOI · Paper page
  16. Mizushima, 2004, In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker, Mol. Biol. Cell, 15, 1101, 10.1091/mbc.e03-09-0704 · DOI · Paper page
  17. Molinari, 2003, Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle, Science, 299, 1397, 10.1126/science.1079474 · DOI · Paper page
  18. Nishida, 2009, Discovery of Atg5/Atg7-independent alternative macroautophagy, Nature, 461, 654, 10.1038/nature08455 · DOI · Paper page
  19. Oda, 2003, EDEM as an acceptor of terminally misfolded glycoproteins released from calnexin, Science, 299, 1394, 10.1126/science.1079181 · DOI · Paper page
  20. Oostra, 2007, Localization and membrane topology of coronavirus nonstructural protein 4: involvement of the early secretory pathway in replication, J. Virol., 81, 12323, 10.1128/JVI.01506-07 · DOI · Paper page
  21. Oostra, 2008, Topology and membrane anchoring of the coronavirus replication complex: not all hydrophobic domains of nsp3 and nsp6 are membrane spanning, J. Virol., 82, 12392, 10.1128/JVI.01219-08 · DOI · Paper page
  22. Prentice, 2004, Coronavirus replication complex formation utilizes components of cellular autophagy, J. Biol. Chem., 279, 10136, 10.1074/jbc.M306124200 · DOI · Paper page
  23. Raaben, 2007, Mouse hepatitis coronavirus replication induces host translational shutoff and mRNA decay, with concomitant formation of stress granules and processing bodies, Cell. Microbiol., 9, 2218, 10.1111/j.1462-5822.2007.00951.x · DOI
  24. Salonen, 2005, Viral RNA replication in association with cellular membranes, Curr. Top. Microbiol. Immunol., 285, 139
  25. Sato, 2007, Mechanisms of COPII vesicle formation and protein sorting, FEBS Lett., 581, 2076, 10.1016/j.febslet.2007.01.091 · DOI · Paper page
  26. Schiller, 1998, Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a, Virology, 242, 288, 10.1006/viro.1997.9010 · DOI
  27. Slot, 2007, Cryosectioning and immunolabeling, Nat. Protoc., 2, 2480, 10.1038/nprot.2007.365 · DOI · Paper page
  28. Snijder, 2006, Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex, J. Virol., 80, 5927, 10.1128/JVI.02501-05 · DOI · Paper page
  29. Soldà, 2006, Consequences of ERp57 deletion on oxidative folding of obligate and facultative clients of the calnexin cycle, J. Biol. Chem., 281, 6219, 10.1074/jbc.M513595200 · DOI
  30. Stertz, 2007, The intracellular sites of early replication and budding of SARS-coronavirus, Virology, 361, 304, 10.1016/j.virol.2006.11.027 · DOI · Paper page
  31. Ulasli, 2010, Qualitative and quantitative ultrastructural analysis of the membrane rearrangements induced by coronavirus, Cell. Microbiol., 12, 844, 10.1111/j.1462-5822.2010.01437.x · DOI · Paper page
  32. Verheije, 2008, Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activation, PLoS Pathog., 4, e1000088, 10.1371/journal.ppat.1000088 · DOI
  33. Waguri, 2009, Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice, Methods Enzymol., 453, 181, 10.1016/S0076-6879(08)04009-3 · DOI · Paper page
  34. Yoshimori, 2008, Toward unraveling membrane biogenesis in mammalian autophagy, Curr. Opin. Cell Biol., 20, 401, 10.1016/j.ceb.2008.03.010 · DOI
  35. Zhao, 2007, Coronavirus replication does not require the autophagy gene ATG5, Autophagy, 3, 581, 10.4161/auto.4782 · DOI · Paper page
  36. Zuber, 2007, EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites, Proc. Natl. Acad. Sci. USA, 104, 4407, 10.1073/pnas.0700154104 · DOI

Cited by

See all 383

Metadata

Authors
Reggiori, F., Monastyrska, I., Verheije, M. H., Calì, T., Ulasli, M., Bianchi, S., Bernasconi, R., de Haan, C. A. M., & Molinari, M.
Venue
Cell Host & Microbe
Publisher
Elsevier BV
Published
2010-06
Volume
7
Issue
6
Pages
500-508
ISSN
1931-3128 (ISSN-L)
DOI
10.1016/j.chom.2010.05.013
OpenAlex
W2043464646
Cited by
383
References
3
FWCI
14.03
Type
journal article
Language
en
Open access
bronze
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Coronaviruses Hijack the LC3-I-Positive EDEMosomes, ER-Derived Vesicles Exporting Short-Lived ERAD Regulators, for Replication. https://app.cochat.ai/papers/10.1016/j.chom.2010.05.013
The paper
Reggiori, F., Monastyrska, I., Verheije, M. H., Calì, T., Ulasli, M., Bianchi, S., Bernasconi, R., de Haan, C. A. M., & Molinari, M. (2010). Coronaviruses Hijack the LC3-I-Positive EDEMosomes, ER-Derived Vesicles Exporting Short-Lived ERAD Regulators, for Replication. Cell Host & Microbe. https://doi.org/10.1016/j.chom.2010.05.013

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.