The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Posttranscriptional m6A Editing of HIV-1 mRNAs Enhances Viral Gene Expression
Open accessAbstract
Summary Covalent addition of a methyl group to the adenosine N6 (m6A) is an evolutionarily conserved and common RNA modification that is thought to modulate several aspects of RNA metabolism. While the presence of multiple m6A editing sites on diverse viral RNAs was reported starting almost 40 years ago, how m6A editing affects virus replication has remained unclear. Here, we used photo-crosslinking-assisted m6A sequencing techniques to precisely map several m6A editing sites on the HIV-1 genome and report that they cluster in the HIV-1 3’ untranslated region (3'UTR). Viral 3'UTR m6A sites or analogous cellular m6A sites strongly enhanced mRNA expression in cis by recruiting the cellular YTHDF m6A “reader” proteins. Reducing YTHDF expression inhibited, while YTHDF overexpression enhanced, HIV-1 protein and RNA expression, and virus replication in CD4+ T cells. These data identify m6A editing, and the resultant recruitment of YTHDF proteins, as major positive regulators of HIV-1 mRNA expression.
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Cites
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“The importance ofm6A is underlined by the fact that this modification is evolutionarily conserved from fungi to plants and animals, and that global inhibition of m6A addition is embryonic lethal in plants, insects, and mammals (Meyer and Jaffrey, 2014; Yue et al., 2015).”
BackgroundInfluentialComprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons
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Cited by
Reading, writing and erasing mRNA methylation
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YTHDF3 facilitates translation and decay of N6-methyladenosine-modified RNA
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RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
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Mettl3-mediated mRNA m6A methylation promotes dendritic cell activation
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Metadata
- Authors
- Kennedy, E. M., Bogerd, H. P., Kornepati, A. V. R., Kang, D., Ghoshal, D., Marshall, J. B., Poling, B. C., Tsai, K., Gokhale, N. S., Horner, S. M., & Cullen, B. R.
- Venue
- Cell Host & Microbe
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 19
- Issue
- 5
- Pages
- 675-685
- ISSN
- 1931-3128 (ISSN-L)
- OpenAlex
- W2336857745
- Cited by
- 378
- References
- 9
- FWCI
- 16.70
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kennedy, E. M., Bogerd, H. P., Kornepati, A. V. R., Kang, D., Ghoshal, D., Marshall, J. B., Poling, B. C., Tsai, K., Gokhale, N. S., Horner, S. M., & Cullen, B. R. (2016). Posttranscriptional m6A Editing of HIV-1 mRNAs Enhances Viral Gene Expression. Cell Host & Microbe. https://doi.org/10.1016/j.chom.2016.04.002
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