Cited by 129Genetically Modified Organisms ResearchMicrofluidic and Capillary Electrophoresis ApplicationsMetabolomics and Mass Spectrometry Studies

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Comparative metabolomic study of transgenic versus conventional soybean using capillary electrophoresis-time-of-flight mass spectrometry

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García-Villalba, Rocio, León, Carlos, Dinelli, Giovanni, Segura-Carretero, Antonio, Fernández-Gutiérrez, Alberto, Garcia-Cañas, Virginia, Cifuentes, Alejandro·2008·Journal of Chromatography A

DOI 10.1016/j.chroma.2008.05.018

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Abstract

In this work, capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF-MS) is proposed to identify and quantify the main metabolites found in transgenic soybean and its corresponding non-transgenic parental line both grown under identical conditions. The procedure includes optimization of metabolites extraction, separation by CE, on-line electrospray-TOF-MS analysis and data evaluation. A large number of extraction procedures and background electrolytes are tested in order to obtain a highly reproducible and sensitive analytical methodology. Using this approach, a large number of metabolites were tentatively identified based on the high mass accuracy provided by TOF-MS analyzer, together with the isotopic pattern and expected electrophoretic mobility of these compounds. In general, the same metabolites and in similar amounts were found in the conventional and transgenic variety. However, significant differences were also observed in some specific cases when the conventional variety was compared with its corresponding transgenic line. The selection of these metabolites as possible biomarkers of transgenic soybean is discussed, although a larger number of samples need to be analyzed in order to validate this point. It is concluded that metabolomic procedures based on CE-MS can open new perspectives in the study of transgenic foods in order to corroborate (or not) the equivalence with their conventional counterparts.

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Authors: García-Villalba, R., León, C., Dinelli, G., Segura-Carretero, A., Fernández-Gutiérrez, A., Garcia-Cañas, V., & Cifuentes, A.
Affiliations: Consejo Superior de Investigaciones Científicas (ES)
University of Bologna (IT)
ORCID: León, Carlos
Dinelli, Giovanni
Cifuentes, Alejandro
Venue: Journal of Chromatography A
Publisher: Elsevier BV
Published: 2008-06
Volume: 1195
Issue: 1-2
Pages: 164-173
ISSN: 0021-9673 (ISSN-L)
DOI: 10.1016/j.chroma.2008.05.018
OpenAlex: W1966413091
Cited by: 129
References: 55
Citations by year: 2025: 2 · 2023: 7 · 2022: 4 · 2021: 5 · 2020: 8 · 2019: 8 · 2018: 5 · 2017: 7 · 2016: 7 · 2015: 8 · 2014: 12 · 2013: 15 · 2012: 9
FWCI: 12.50
Type: journal article
Language: en
Open access: green
hdl.handle.net
Licence: cc-by-nc-nd
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Journal of Chromatography A (publishedVersion)
PubMed (publishedVersion)
Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
Institutional Repository of the University of Granada (University of Granada) (submittedVersion, cc-by-nc-nd)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords: transgenic soybean, metabolomics, metabolite extraction, supporting electrolyte
MeSH terms: Glycine max, Electrophoresis, Capillary, Spectrometry, Mass, Electrospray Ionization, Plant Preparations, Plants, Genetically Modified

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Comparative metabolomic study of transgenic versus conventional soybean using capillary electrophoresis-time-of-flight mass spectrometry

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