The DOI matches its Crossref record; 205 of the 205 references it cites were found in a registry or an index.
Modern isolation and separation techniques for extracellular vesicles
Open accessAbstract
Extracellular vesicles (EVs) are heterogenous membrane-bound vesicles released from various origins. EVs play a crucial role in cellular communication and mediate several physiological and pathological processes, highlighting their potential therapeutic and diagnostic applications. Due to the rapid increase in interests and needs to elucidate EV properties and functions, numerous isolation and separation approaches for EVs have been developed to overcome limitations of conventional techniques, such as ultracentrifugation. This review focuses on recently emerging and modern EV isolation and separation techniques, including size-, charge-, and affinity-based techniques while excluding ultracentrifugation and precipitation-based techniques due to their multiple limitations. The advantages and drawbacks of each technique are discussed together with insights into their applications. Emerging approaches all share similar features in terms of being time-effective, easy-to-operate, and capable of providing EVs with suitable and desirable purity and integrity for applications of interest. Combination and hyphenation of techniques have been used for EV isolation and separation to yield EVs with the best quality. The most recent development using an automated on-line system including selective affinity-based trapping unit and asymmetrical flow field-flow fractionation allows reliable isolation and fractionation of EV subpopulations from human plasma.
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Cites
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Cited by
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Exosome Processing and Characterization Approaches for Research and Technology Development
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Recent advances in extracellular vesicles for therapeutic cargo delivery
Kim, H. I., Park, J., Zhu, Y., Wang, X., Han, Y., & Zhang, D. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01201-6 · Cited by 505
Metadata
- Authors
- Liangsupree, T., Multia, E., & Riekkola, M.-L.
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 2021-01
- Volume
- 1636
- Pages
- 461773
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W3109744624
- Cited by
- 512
- References
- 205
- FWCI
- 16.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Liangsupree, T., Multia, E., & Riekkola, M.-L. (2021). Modern isolation and separation techniques for extracellular vesicles. Journal of Chromatography A. https://doi.org/10.1016/j.chroma.2020.461773
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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