Exosome Processing and Characterization Approaches for Research and Technology Development

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells

Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834 · see citing works

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099 · see citing works

The biology <b>,</b> function <b>,</b> and biomedical applications of exosomes

Kalluri, R., & LeBleu, V. S. (2020) · Science · doi:10.1126/science.aau6977 · Cited by 10,770 · see citing works

α-Synuclein in Lewy bodies

Spillantini, M. G., Schmidt, M. L., Lee, V. M.-Y., Trojanowski, J. Q., Jakes, R., & Goedert, M. (1997) · Nature · doi:10.1038/42166 · Cited by 8,382 · see citing works

Extracellular vesicles: Exosomes, microvesicles, and friends

Raposo, G., & Stoorvogel, W. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201211138 · Cited by 8,099 · see citing works

Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles

Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186 · see citing works

Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766 · see citing works

Membrane vesicles as conveyors of immune responses

Théry, C., Ostrowski, M., & Segura, É. (2009) · Nature reviews. Immunology · doi:10.1038/nri2567 · Cited by 3,869 · see citing works

Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes

Trajković, K., Hsu, C., Chiantia, S., Rajendran, L., Wenzel, D., Wieland, F., Schwille, P., Brügger, B., & Simons, M. (2008) · Science · doi:10.1126/science.1153124 · Cited by 3,639 · see citing works

B lymphocytes secrete antigen-presenting vesicles.

Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996) · The Journal of experimental medicine · doi:10.1084/jem.183.3.1161 · Cited by 3,546 · see citing works

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426 · see citing works

Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes)

Johnstone, R. M., Adam, M., Hammond, J. R., Orr, L., & Turbide, C. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48095-7 · Cited by 2,931 · see citing works

Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response

Chen, G., Huang, A. C., Zhang, W., Zhang, G., Wu, M., Xu, W., Yu, Z., Yang, J., Wang, B., Sun, H., et al. (2018) · Nature · doi:10.1038/s41586-018-0392-8 · Cited by 2,813 · see citing works

Rab27a and Rab27b control different steps of the exosome secretion pathway

Ostrowski, M., Carmo, N., Krumeich, S., Fanget, I., Raposo, G., Savina, A., Moita, C. F., Schauer, K., Hume, A. N., Freitas, R. P., et al. (2009) · Nature Cell Biology · doi:10.1038/ncb2000 · Cited by 2,761 · see citing works

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

Kamerkar, S., LeBleu, V. S., Sugimoto, H., Yang, S., Ruivo, C. F., Melo, S. A., Lee, J. J., & Kalluri, R. (2017) · Nature · doi:10.1038/nature22341 · Cited by 2,639 · see citing works

The Low-Density Lipoprotein Pathway and its Relation to Atherosclerosis

Goldstein, L. J., & Brown, S. M. (1977) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.46.070177.004341 · Cited by 2,455 · see citing works

Current knowledge on exosome biogenesis and release

Hessvik, N. P., & Llorente, A. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-017-2595-9 · Cited by 2,362 · see citing works

Exosomes – vesicular carriers for intercellular communication

Simons, M., & Raposo, G. (2009) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2009.03.007 · Cited by 2,351 · see citing works

Exosomes: biogenesis, biologic function and clinical potential

Zhang, Y., Liu, Y., Liu, H., & Tang, W. H. (2019) · Cell &amp; Bioscience · doi:10.1186/s13578-019-0282-2 · Cited by 2,119 · see citing works

Progress in Exosome Isolation Techniques

Li, P., Kaslan, M., Lee, S. H., Yao, J. D., & Gao, Z. (2017) · Theranostics · doi:10.7150/thno.18133 · Cited by 1,970 · see citing works

Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials

Vlassov, A. V., Magdaleno, S. M., Setterquist, R. A., & Conrad, R. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2012.03.017 · Cited by 1,936 · see citing works

Critical evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates

Filipe, V., Hawe, A., & Jiskoot, W. (2010) · Pharmaceutical Research · doi:10.1007/s11095-010-0073-2 · Cited by 1,885 · see citing works

RNA delivery by extracellular vesicles in mammalian cells and its applications

O’Brien, K., Breyne, K., Ughetto, S., Laurent, L. C., & Breakefield, X. O. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0251-y · Cited by 1,811 · see citing works

Optimized exosome isolation protocol for cell culture supernatant and human plasma

Lobb, R. J., Becker, M., Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,725 · see citing works

New Technologies for Analysis of Extracellular Vesicles

Shao, H., Im, H., Castro, C. M., Breakefield, X. O., Weissleder, R., & Lee, H. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00534 · Cited by 1,672 · see citing works

Double-stranded DNA in exosomes: a novel biomarker in cancer detection

Thakur, B. K., Zhang, H., Becker, A., Matei, I. R., Huang, Y., Costa‐Silva, B., Zheng, Y., Hoshino, A., Brazier, H., Xiang, J., et al. (2014) · Cell Research · doi:10.1038/cr.2014.44 · Cited by 1,592 · see citing works

Activated Platelets Release Two Types of Membrane Vesicles: Microvesicles by Surface Shedding and Exosomes Derived From Exocytosis of Multivesicular Bodies and -Granules

Heijnen, H. F. G., Schiel, A. E., Fijnheer, R., Geuze, H. J., & Sixma, J. J. (1999) · Blood · doi:10.1182/blood.v94.11.3791 · Cited by 1,488 · see citing works

Cell-Surface Calreticulin Initiates Clearance of Viable or Apoptotic Cells through trans-Activation of LRP on the Phagocyte

Gardai, S. J., McPhillips, K. A., Frasch, S. C., Janssen, W. J., Starefeldt, A., Murphy-Ullrich, J. E., Bratton, D. L., Oldenborg, P.-A., Michalak, M., & Henson, P. M. (2005) · Cell · doi:10.1016/j.cell.2005.08.032 · Cited by 1,473 · see citing works

ExoCarta: A Web-Based Compendium of Exosomal Cargo

Keerthikumar, S., Chisanga, D., Ariyaratne, D., Saffar, H. A., Anand, S., Zhao, K., Samuel, M., Pathan, M., Jois, M., Chilamkurti, N., et al. (2015) · Journal of Molecular Biology · doi:10.1016/j.jmb.2015.09.019 · Cited by 1,447 · see citing works

Ectosomes and exosomes: shedding the confusion between extracellular vesicles

Cocucci, E., & Meldolesi, J. (2015) · Trends in Cell Biology · doi:10.1016/j.tcb.2015.01.004 · Cited by 1,435 · see citing works

Biogenesis of extracellular vesicles (EV): exosomes, microvesicles, retrovirus-like vesicles, and apoptotic bodies

Akers, J. C., Gonda, D., Kim, R., Carter, B. S., & Chen, C. C. (2013) · Journal of Neuro-Oncology · doi:10.1007/s11060-013-1084-8 · Cited by 1,423 · see citing works

Tetraspanins in Extracellular Vesicle Formation and Function

Andreu, Z., & Yáñez-Mó, M. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00442 · Cited by 1,401 · see citing works

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis

Dragovic, R., Gardiner, C., Brooks, A. S., Tannetta, D., Ferguson, D. J. P., Hole, P., Carr, B., Redman, C. W. G., Harris, A. L., Dobson, P. J., et al. (2011) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2011.04.003 · Cited by 1,368 · see citing works

Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation

Kalra, H., Simpson, R. J., Ji, H., Aikawa, E., Altevogt, P., Askenase, P., Bond, V. C., Borràs, F. E., Breakefield, X., Budnik, V., et al. (2012) · PLoS Biology · doi:10.1371/journal.pbio.1001450 · Cited by 1,367 · see citing works

Alzheimer’s disease β-amyloid peptides are released in association with exosomes

Rajendran, L., Honsho, M., Zahn, T. R., Keller, P., Geiger, K. D., Verkade, P., & Simons, K. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0603838103 · Cited by 1,343 · see citing works

Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor

Im, H., Shao, H., Park, Y. I., Peterson, V. M., Castro, C. M., Weissleder, R., & Lee, H. (2014) · Nature Biotechnology · doi:10.1038/nbt.2886 · Cited by 1,324 · see citing works

Using Raman spectroscopy to characterize biological materials

Butler, H. J., Ashton, L., Bird, B., Cinque, G., Curtis, K., Dorney, J., Esmonde-White, K., Fullwood, N. J., Gardner, B., Martin-Hirsch, P. L., et al. (2016) · Nature Protocols · doi:10.1038/nprot.2016.036 · Cited by 1,309 · see citing works

Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study

Zaborowski, M. P., Balaj, L., Breakefield, X. O., & Lai, C. P. (2015) · BioScience · doi:10.1093/biosci/biv084 · Cited by 1,272 · see citing works

Isolation of Extracellular Vesicles: General Methodologies and Latest Trends

Konoshenko, M. Y., Lekchnov, E. A., Vlassov, A. V., & Laktionov, P. P. (2018) · BioMed Research International · doi:10.1155/2018/8545347 · Cited by 1,257 · see citing works

Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans

Wang, G., Zhu, J., Zhang, J., Li, Q., Li, Y., He, J., Qin, Y., & Jing, Q. (2010) · European Heart Journal · doi:10.1093/eurheartj/ehq013 · Cited by 1,168 · see citing works

Exosomes in cancer development, metastasis, and drug resistance: a comprehensive review

Azmi, A. S., Bao, B., & Sarkar, F. H. (2013) · Cancer and Metastasis Reviews · doi:10.1007/s10555-013-9441-9 · Cited by 1,159 · see citing works

Cell-produced α-synuclein is secreted in a calcium-dependent manner by exosomes and impacts neuronal survival

Emmanouilidou, E., Melachroinou, K., Roumeliotis, T., Garbis, S. D., Ntzouni, M., Margaritis, L. H., Stefanis, L., & Vekrellis, K. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.5699-09.2010 · Cited by 1,115 · see citing works

Quantitative and stoichiometric analysis of the microRNA content of exosomes

Chevillet, J. R., Kang, Q., Ruf, I. K., Briggs, H., Vojtech, L. N., Hughes, S. M., Cheng, H. H., Arroyo, J. D., Meredith, E. K., Gallichotte, E. N., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1408301111 · Cited by 1,105 · see citing works

Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey

Gardiner, C., Vizio, D. D., Sahoo, S., Théry, C., Witwer, K. W., Wauben, M. H. M., & Hill, A. F. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.32945 · Cited by 1,075 · see citing works

Neuroinvasion of SARS-CoV-2 in human and mouse brain

Song, E., Zhang, C., Israelow, B., Lu-Culligan, A., Prado, A. V., Skriabine, S., Lu, P., Weizman, O.-E., Liu, F., Dai, Y., et al. (2020) · doi:10.1101/2020.06.25.169946 · Cited by 1,064 · see citing works

Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics

Yang, D., Zhang, W., Zhang, H., Zhang, F., Chen, L., Ma, L., Larcher, L. M., Chen, S., Liu, N., Zhao, Q., et al. (2020) · Theranostics · doi:10.7150/thno.41580 · Cited by 1,048 · see citing works

Lipids in exosomes: current knowledge and the way forward

Skotland, T., Sandvig, K., & Llorente, A. (2017) · Progress in Lipid Research · doi:10.1016/j.plipres.2017.03.001 · Cited by 1,030 · see citing works

Methodological Guidelines to Study Extracellular Vesicles

Coumans, F. A. W., Brisson, A. R., Buzás, E. I., Dignat‐George, F., Drees, E. E. E., Andaloussi, S. E., Emanueli, C., Gąsecka, A., Hendrix, A., Hill, A. F., et al. (2017) · Circulation Research · doi:10.1161/circresaha.117.309417 · Cited by 1,024 · see citing works

Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

Crescitelli, R., Lässer, C., Szabó, T. G., Kittel, Á., Eldh, M., Dianzani, I., Buzás, E. I., & Lötvall, J. O. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20677 · Cited by 996 · see citing works

Exosome-associated tau is secreted in tauopathy models and is selectively phosphorylated in cerebrospinal fluid in early Alzheimer disease

Saman, S., Kim, W., Raya, M., Visnick, Y., Miro, S., Saman, S., Jackson, B., McKee, A. C., Alvarez, V. E., Lee, N. C. Y., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.277061 · Cited by 985 · see citing works

Systemic Administration of Exosomes Released from Mesenchymal Stromal Cells Promote Functional Recovery and Neurovascular Plasticity After Stroke in Rats

Xin, H., Li, Y., Cui, Y., Yang, J. J., Zhang, Z. G., & Chopp, M. (2013) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2013.152 · Cited by 985 · see citing works

Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression

Willms, E., Cabañas, C., Mäger, I., Wood, M. J. A., & Vader, P. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00738 · Cited by 982 · see citing works

Cardiac fibroblast–derived microRNA passenger strand-enriched exosomes mediate cardiomyocyte hypertrophy

Bang, C., Bátkai, S., Dangwal, S., Gupta, S. K., Foinquinos, A., Holzmann, A., Just, A., Remke, J., Zimmer, K., Zeug, A., et al. (2014) · Journal of Clinical Investigation · doi:10.1172/jci70577 · Cited by 979 · see citing works

Isolation of exosomes from whole blood by integrating acoustics and microfluidics

Wu, M., Ouyang, Y., Wang, Z., Zhang, R., Huang, P., Chen, C., Li, H., Li, P., Quinn, D., Dao, M., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1709210114 · Cited by 958 · see citing works

Exosomes and Ectosomes in Intercellular Communication

Meldolesi, J. (2018) · Current Biology · doi:10.1016/j.cub.2018.01.059 · Cited by 944 · see citing works

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling

Deun, J. V., Mestdagh, P., Sormunen, R. T., Cocquyt, V., Vermaelen, K., Vandesompele, J., Bracke, M., Wever, O. D., & Hendrix, A. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24858 · Cited by 941 · see citing works

Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy

Lee, Y., Andaloussi, S. E., & Wood, M. J. A. (2012) · Human Molecular Genetics · doi:10.1093/hmg/dds317 · Cited by 936 · see citing works

Apoptosis and apoptotic body: disease message and therapeutic target potentials

Xu, X., Lai, Y., & Hua, Z. (2018) · Bioscience Reports · doi:10.1042/bsr20180992 · Cited by 923 · see citing works

Exosomes: A Common Pathway for a Specialized Function

van Niel, G., Porto-Carreiro, I., Simoes, S., & Raposo, G. (2006) · The Journal of Biochemistry · doi:10.1093/jb/mvj128 · Cited by 922 · see citing works

ARF6-Regulated Shedding of Tumor Cell-Derived Plasma Membrane Microvesicles

Muralidharan-Chari, V., Clancy, J., Plou, C., Romao, M., Chavrier, P., Raposo, G., & D'Souza-Schorey, C. (2009) · Current Biology · doi:10.1016/j.cub.2009.09.059 · Cited by 912 · see citing works

A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

Sidhom, K., Obi, P. O., & Saleem, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21186466 · Cited by 908 · see citing works

Exosomes as new vesicular lipid transporters involved in cell–cell communication and various pathophysiologies

Record, M., Carayon, K., Poirot, M., & Silvente‐Poirot, S. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.10.004 · Cited by 853 · see citing works

Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy

Sokolova, V., Ludwig, A., Hornung, S., Rotan, O., Horn, P. A., Epple, M., & Giebel, B. (2011) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2011.05.013 · Cited by 853 · see citing works

Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell‐free blood

Cheng, L., Sharples, R. A., Scicluna, B. J., & Hill, A. F. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.23743 · Cited by 840 · see citing works

How pure are your vesicles?

Webber, J., & Clayton, A. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.19861 · Cited by 817 · see citing works

Dendritic cell-derived exosomes as maintenance immunotherapy after first line chemotherapy in NSCLC

Besse, B., Charrier, M., Lapierre, V., Dansin, É., Lantz, O. J., Planchard, D., Chevalier, T. L., Livartoski, A., Barlési, F., Laplanche, A., et al. (2015) · OncoImmunology · doi:10.1080/2162402x.2015.1071008 · Cited by 802 · see citing works

An Apoptotic ‘Eat Me’ Signal: Phosphatidylserine Exposure

Segawa, K., & Nagata, S. (2015) · Trends in Cell Biology · doi:10.1016/j.tcb.2015.08.003 · Cited by 778 · see citing works

Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis

Fitzner, D., Schnaars, M., van Rossum, D., Krishnamoorthy, G., Dibaj, P., Bakhti, M., Regen, T., Hanisch, U.-K., & Simons, M. (2011) · Journal of Cell Science · doi:10.1242/jcs.074088 · Cited by 775 · see citing works

Molecular lipidomics of exosomes released by PC-3 prostate cancer cells

Llorente, A., Skotland, T., Sylvänne, T., Kauhanen, D., Róg, T., Orłowski, A., Vattulainen, I., Ekroos, K., & Sandvig, K. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.04.011 · Cited by 771 · see citing works

Next generation sequencing technology: Advances and applications

Buermans, H. P. J., & den Dunnen, J. T. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2014.06.015 · Cited by 761 · see citing works

Tuberculosis-associated immune reconstitution inflammatory syndrome: case definitions for use in resource-limited settings

Meintjes, G., Lawn, S. D., Scano, F., Maartens, G., French, M. A., Worodria, W. O., Elliott, J., Murdoch, D. M., Wilkinson, R. J., Seyler, C., et al. (2008) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(08)70184-1 · Cited by 746 · see citing works

Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration

Arraud, N., Linares, R., Tan, S., Gounou, C., Pasquet, J., Mornet, S., & Brisson, A. R. (2014) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12554 · Cited by 742 · see citing works

Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmission

Alvarez‐Erviti, L., Seow, Y., Schapira, A. H. V., Gardiner, C., Sargent, I. L., Wood, M. J. A., & Cooper, J. A. D. (2011) · Neurobiology of Disease · doi:10.1016/j.nbd.2011.01.029 · Cited by 741 · see citing works

Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction

Barile, L., Lionetti, V., Cervio, E., Matteucci, M., Gherghiceanu, M., Popescu, L. M., Torre, T., Siclari, F., Moccetti, T., & Vassalli, G. (2014) · Cardiovascular Research · doi:10.1093/cvr/cvu167 · Cited by 733 · see citing works

Packaging and transfer of mitochondrial DNA via exosomes regulate escape from dormancy in hormonal therapy-resistant breast cancer

Sansone, P., Savini, C., Kurelac, I., Chang, Q., Amato, L. B., Strillacci, A., Степанова, А., Iommarini, L., Mastroleo, C., Daly, L., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1704862114 · Cited by 732 · see citing works

Exosomal lipid composition and the role of ether lipids and phosphoinositides in exosome biology

Skotland, T., Hessvik, N. P., Sandvig, K., & Llorente, A. (2019) · Journal of Lipid Research · doi:10.1194/jlr.r084343 · Cited by 730 · see citing works

A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods

Greening, D. W., Xu, R., Ji, H. L., Tauro, B. J., & Simpson, R. J. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2550-6_15 · Cited by 723 · see citing works

STED super-resolved microscopy

Vicidomini, G., Bianchini, P., & Diaspro, A. (2018) · Nature Methods · doi:10.1038/nmeth.4593 · Cited by 720 · see citing works

ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles

Rider, M. A., Hurwitz, S. N., & Meckes, D. G. (2016) · Scientific Reports · doi:10.1038/srep23978 · Cited by 699 · see citing works

Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol

Livshits, M. A., Khomyakova, E. B., Evtushenko, E. G., Лазарев, В. Н., Kulemin, N. A., Semina, S. E., Generozov, E. V., & Govorun, V. M. (2015) · Scientific Reports · doi:10.1038/srep17319 · Cited by 692 · see citing works

Characterization of magnetic nanoparticle by dynamic light scattering

Lim, J., Yeap, S. P., Che, H. X., & Low, S. C. (2013) · Nanoscale Research Letters · doi:10.1186/1556-276x-8-381 · Cited by 689 · see citing works

Application of exosomes as liquid biopsy in clinical diagnosis

Zhou, B., Xu, K., Zheng, X., Chen, T., Wang, J., Song, Y., Shao, Y., & Zheng, S. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00258-9 · Cited by 685 · see citing works

Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

Baranyai, T., Herczeg, K., Onódi, Z., Voszka, I., Módos, K., Marton, N., Nagy, G., Mäger, I., Wood, M. J. A., Andaloussi, S. E., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0145686 · Cited by 677 · see citing works

Plasma exosomal α-synuclein is likely CNS-derived and increased in Parkinson’s disease

Shi, M., Liu, C., Cook, T. J., Bullock, K. M., Zhao, Y., Ginghina, C., Li, Y., Aro, P., Dator, R., He, C., et al. (2014) · Acta Neuropathologica · doi:10.1007/s00401-014-1314-y · Cited by 676 · see citing works

ESCRTs are everywhere

Hurley, J. H. (2015) · The EMBO Journal · doi:10.15252/embj.201592484 · Cited by 668 · see citing works

Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes

Kanwar, S. S., Dunlay, C. J., Simeone, D. M., & Nagrath, S. (2014) · Lab on a Chip · doi:10.1039/c4lc00136b · Cited by 663 · see citing works

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

Contreras-Naranjo, J. C., Wu, H., & Ugaz, V. M. (2017) · Lab on a Chip · doi:10.1039/c7lc00592j · Cited by 650 · see citing works

Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications

Patel, G. K., Khan, M. A., Zubair, H., Srivastava, S. K., Khushman, M. M., Singh, S., & Singh, A. P. (2019) · Scientific Reports · doi:10.1038/s41598-019-41800-2 · Cited by 645 · see citing works

Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry

Tian, Y., Gong, M., Hu, Y., Liu, H., Zhang, W., Zhang, M., Hu, X., Aubert, D., Zhu, S., Wu, L., et al. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1697028 · Cited by 643 · see citing works

Cellular stress conditions are reflected in the protein and RNA content of endothelial cell‐derived exosomes

de Jong, O. G., Verhaar, M. C., Chen, Y., Vader, P., Gremmels, H., Posthuma, G., Schiffelers, R. M., Guček, M., & van Balkom, B. W. M. (2012) · Journal of Extracellular Vesicles · doi:10.3402/jev.v1i0.18396 · Cited by 634 · see citing works

Quantitation of targets for PCR by use of limiting dilution.

Sykes, P. J., Neoh, S. H., Brisco, M. J., Hughes, E., Condon, J. C., & Morley, A. A. (1992) · PubMed · Cited by 621 · see citing works

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Donoso‐Quezada, J., Ayala‐Mar, S., & González‐Valdez, J. (2021) · Traffic · doi:10.1111/tra.12803 · Cited by 605 · see citing works

Exosome Mediated Delivery of miR-124 Promotes Neurogenesis after Ischemia

Yang, J., Zhang, X., Chen, X., Wang, L., & Yang, G. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.04.010 · Cited by 592 · see citing works

Biology and biogenesis of shed microvesicles

Tricarico, C. J., Clancy, J. W., & D’Souza‐Schorey, C. (2016) · Small GTPases · doi:10.1080/21541248.2016.1215283 · Cited by 588 · see citing works

Selective Release of MicroRNA Species from Normal and Malignant Mammary Epithelial Cells

Pigati, L., Yaddanapudi, S. C., Iyengar, R., Kim, D.-J., Hearn, S., Danforth, D. N., Hastings, M. L., & Duelli, D. M. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013515 · Cited by 583 · see citing works

Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers

Alvarez, M. L., Khosroheidari, M., Ravi, R. K., & DiStefano, J. K. (2012) · Kidney International · doi:10.1038/ki.2012.256 · Cited by 578 · see citing works

Human Tumor-Derived Exosomes Down-Modulate NKG2D Expression

Clayton, A., Mitchell, J. P., Court, J., Linnane, S. J., Mason, M. D., & Tabi, Z. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.180.11.7249 · Cited by 574 · see citing works

The Methods of Choice for Extracellular Vesicles (EVs) Characterization

Szatanek, R., Baj‐Krzyworzeka, M., Zimoch, J., Lekka, M., Siedlar, M., & Baran, J. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18061153 · Cited by 571 · see citing works

Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane

Booth, A. M., Fang, Y., Fallon, J. K., Yang, J.-M., Hildreth, J. E. K., & Gould, S. J. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200508014 · Cited by 562 · see citing works

Chemical Basis of the Sulfo-phospho-vanillin Reaction for Estimating Total Serum Lipids

Knight, J. A., Anderson, S., & Rawle, J. M. (1972) · Clinical Chemistry · doi:10.1093/clinchem/18.3.199 · Cited by 556 · see citing works

Altered microRNA profiles in cerebrospinal fluid exosome in Parkinson disease and Alzheimer disease

Gui, Y., Liu, H., Zhang, L., Lv, W., & Hu, X. (2015) · Oncotarget · doi:10.18632/oncotarget.6158 · Cited by 552 · see citing works

TI-VAMP/VAMP7 and VAMP3/cellubrevin: two v-SNARE proteins involved in specific steps of the autophagy/multivesicular body pathways

Fader, C. M., Sánchez, D. G., Mestre, M. B., & Colombo, M. I. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2009.09.011 · Cited by 544 · see citing works

HIV Nef is Secreted in Exosomes and Triggers Apoptosis in Bystander CD4+ T Cells

Lenassi, M., Cagney, G., Liao, M., Vaupotič, T., Bartholomeeusen, K., Cheng, Y., Krogan, N. J., Plemenitaš, A., & Peterlin, B. M. (2009) · Traffic · doi:10.1111/j.1600-0854.2009.01006.x · Cited by 542 · see citing works

Exosomes as intercellular signalosomes and pharmacological effectors

Record, M., Subra, C., Silvente‐Poirot, S., & Poirot, M. (2011) · Biochemical Pharmacology · doi:10.1016/j.bcp.2011.02.011 · Cited by 539 · see citing works

Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Busatto, S., Vilanilam, G. K., Ticer, T. D., Lin, W., Dickson, D. W., Shapiro, S. A., Bergese, P., & Wolfram, J. (2018) · Cells · doi:10.3390/cells7120273 · Cited by 538 · see citing works

Elucidation of Exosome Migration Across the Blood–Brain Barrier Model In Vitro

Chen, C. C., Liu, L., Ma, F., Wong, C. W., Guo, X. E., Chacko, J. V., Farhoodi, H. P., Zhang, S. X., Zimak, J., Ségaliny, A., et al. (2016) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-016-0458-3 · Cited by 537 · see citing works

Isolation and characterization of exosomes for cancer research

Zhu, L., Sun, H., Wang, S., Huang, S., Zheng, Y., Wang, C., Hu, B., Qin, W., Zou, T., Fu, Y., et al. (2020) · Journal of Hematology & Oncology · doi:10.1186/s13045-020-00987-y · Cited by 529 · see citing works

Modern isolation and separation techniques for extracellular vesicles

Liangsupree, T., Multia, E., & Riekkola, M. (2020) · Journal of Chromatography A · doi:10.1016/j.chroma.2020.461773 · Cited by 520 · see citing works

Technical challenges of working with extracellular vesicles

Ramirez, M. I., de Amorim, M. G., Gadelha, C., Milic, I., Welsh, J. A., Freitas, V. M., Nawaz, M., Akbar, N., Couch, Y., Makin, L., et al. (2017) · Nanoscale · doi:10.1039/c7nr08360b · Cited by 518 · see citing works

Isolation and Characterization of RNA-Containing Exosomes

Lässer, C., Eldh, M., & Lötvall, J. (2012) · Journal of Visualized Experiments · doi:10.3791/3037 · Cited by 509 · see citing works

Early-Stage Lung Cancer Diagnosis by Deep Learning-Based Spectroscopic Analysis of Circulating Exosomes

Shin, H., Oh, S., Hong, S., Kang, M.-S., Kang, D., Ji, Y.-G., Choi, B. H., Kang, K., Jeong, H., Park, Y., et al. (2020) · ACS Nano · doi:10.1021/acsnano.9b09119 · Cited by 508 · see citing works

Inhibition of miR-15 Protects Against Cardiac Ischemic Injury

Hullinger, T. G., Montgomery, R. L., Seto, A. G., Dickinson, B., Semus, H. M., Lynch, J. M., Dalby, C. M., Robinson, K., Stack, C., Latimer, P. A., et al. (2011) · Circulation Research · doi:10.1161/circresaha.111.244442 · Cited by 502 · see citing works

Technologies and Standardization in Research on Extracellular Vesicles

Gandham, S. K., Su, X., Wood, J., Nocera, A. L., Alli, S. C., Milane, L. S., Zimmerman, A. J., Amiji, M. M., & Ivanov, A. R. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.05.012 · Cited by 494 · see citing works

Identification and proteomic profiling of exosomes in human cerebrospinal fluid

Street, J. M., Barran, P. E., Mackay, C. L., Weidt, S. K., Balmforth, C., Walsh, T. S., Chalmers, R. T. A., Webb, D. J., & Dear, J. W. (2012) · Journal of Translational Medicine · doi:10.1186/1479-5876-10-5 · Cited by 491 · see citing works

Apoptotic Bodies: Particular Extracellular Vesicles Involved in Intercellular Communication

Battistelli, M., & Falcieri, E. (2020) · Biology · doi:10.3390/biology9010021 · Cited by 479 · see citing works

Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows

Liu, C., Guo, J., Tian, F., Yang, N., Yan, F., Ding, Y., Wei, J., Hu, G., Nie, G., & Sun, J. (2017) · ACS Nano · doi:10.1021/acsnano.7b02277 · Cited by 477 · see citing works

Integrated Magneto–Electrochemical Sensor for Exosome Analysis

Jeong, S., Park, J., Pathania, D., Castro, C. M., Weissleder, R., & Lee, H. (2016) · ACS Nano · doi:10.1021/acsnano.5b07584 · Cited by 468 · see citing works

Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator

Cheruvanky, A., Zhou, H., Pisitkun, T., et al. (2007) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00434.2006 · Cited by 461 · see citing works

Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum

Tang, Y., Huang, Y., Zheng, L., Qin, S., Xu, X.-P., An, T., Xu, Y., Wu, Y.-S., Hu, X., Ping, B., et al. (2017) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2017.3080 · Cited by 455 · see citing works

Extracellular vesicle isolation methods: rising impact of size-exclusion chromatography

Tortajada, M., Gálvez‐Montón, C., Bayés‐Genís, A., Roura, S., & Borràs, F. E. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03071-y · Cited by 443 · see citing works

Challenges in Exosome Isolation and Analysis in Health and Disease

Ludwig, N., Whiteside, T. L., & Reichert, T. E. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20194684 · Cited by 430 · see citing works

Induction of α-synuclein aggregate formation by CSF exosomes from patients with Parkinson’s disease and dementia with Lewy bodies

Stuendl, A., Kunadt, M., Kruse, N., Bartels, C., Moebius, W., Danzer, K. M., Mollenhauer, B., & Schneider, A. (2016) · Brain · doi:10.1093/brain/awv346 · Cited by 428 · see citing works

Exosomes in cancer development and clinical applications

Tai, Y., Chen, K., Hsieh, J., & Shen, T. (2018) · Cancer Science · doi:10.1111/cas.13697 · Cited by 426 · see citing works

Raft ceramide in molecular medicine

Gulbins, E., & Kolesnick, R. (2003) · Oncogene · doi:10.1038/sj.onc.1207146 · Cited by 425 · see citing works

Single vs. swarm detection of microparticles and exosomes by flow cytometry

van der Pol, E., van Gemert, M. J. C., Sturk, A., Nieuwland, R., & van Leeuwen, T. G. J. M. (2012) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2012.04683.x · Cited by 423 · see citing works

Exosome removal as a therapeutic adjuvant in cancer

Marleau, A. M., Chen, C.-S., Joyce, J. A., & Tullis, R. H. (2012) · Journal of Translational Medicine · doi:10.1186/1479-5876-10-134 · Cited by 412 · see citing works

Cardiomyocytes mediate anti-angiogenesis in type 2 diabetic rats through the exosomal transfer of miR-320 into endothelial cells

Wang, X., Huang, W. C., Liu, G., Cai, W., Millard, R. W., Wang, Y., Chang, J., Peng, T., & Fan, G. (2014) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2014.05.001 · Cited by 406 · see citing works

Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer

Pospíchalová, V., Svoboda, J., Dave, Z., Kotrbova, A. V., Kaiser, K., Klemová, D., Ilkovics, L., Hampl, A., Crha, I., Jandáková, E., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.25530 · Cited by 388 · see citing works

Regulation of exosome production and cargo sorting

Wei, H., Chen, Q., Lin, L., Sha, C., Li, T., Liu, Y., Yin, X., Xu, Y., Chen, L., Gao, W., et al. (2021) · International Journal of Biological Sciences · doi:10.7150/ijbs.53671 · Cited by 385 · see citing works

Exosome isolation: a microfluidic road-map

Liga, A., Vliegenthart, A. D. B., Oosthuyzen, W., Dear, J. W., & Kersaudy-Kerhoas, M. (2015) · Lab on a Chip · doi:10.1039/c5lc00240k · Cited by 384 · see citing works

Strategies for Isolation of ExosomesPreprint

Zeringer, E., Barta, T. J., Li, M., & Vlassov, A. V. (2015) · Cold Spring Harbor Protocols · doi:10.1101/pdb.top074476 · Cited by 383 · see citing works

Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers

Li, M., Zeringer, E., Barta, T. J., Schageman, J. J., Cheng, A., & Vlassov, A. V. (2014) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2013.0502 · Cited by 382 · see citing works

The fractionation of protein mixtures by linear polymers of high molecular weight

Polson, A., Potgieter, G. M., Largier, J. F., Mears, G. E. F., & Joubert, F. J. (1964) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(64)90438-6 · Cited by 381 · see citing works

Comparative analysis of discrete exosome fractions obtained by differential centrifugation

Jeppesen, D. K., Hvam, M. L., Primdal-Bengtson, B., Boysen, A. T., Whitehead, B. J., Dyrskjøt, L., Orntoft, T. F., Howard, K. A., & Ostenfeld, M. S. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.25011 · Cited by 369 · see citing works

Exosome isolation from distinct biofluids using precipitation and column-based approaches

Martins, T. S., Catita, J. A. M., Rosa, I. M., Silva, O. A. B. D. C. E., & Henriques, A. G. (2018) · PLoS ONE · doi:10.1371/journal.pone.0198820 · Cited by 369 · see citing works

Exosomes: improved methods to characterize their morphology, RNA content, and surface protein biomarkers

Wu, Y., Deng, W., & Klinke, D. J. (2015) · The Analyst · doi:10.1039/c5an00688k · Cited by 368 · see citing works

Exosome-SIRPα, a CD47 blockade increases cancer cell phagocytosis

Koh, E., Lee, E. J., Nam, G., Hong, Y., Cho, E., Yang, Y., & Kim, I. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.01.004 · Cited by 365 · see citing works

Exosomes in Cancer Diagnostics

Soung, Y. H., Ford, S. K., Zhang, V., & Chung, J. (2017) · Cancers · doi:10.3390/cancers9010008 · Cited by 365 · see citing works

Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

Wang, Z., Wu, H., Fine, D., Schmulen, J., Hu, Y., Godin, B., Zhang, J. X. J., & Liu, X. (2013) · Lab on a Chip · doi:10.1039/c3lc41343h · Cited by 358 · see citing works

On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets

Beer, N. R., Hindson, B. J., Wheeler, E. K., Hall, S. B., Rose, K. A., Kennedy, I. M., & Colston, B. W. (2007) · Analytical Chemistry · doi:10.1021/ac701809w · Cited by 358 · see citing works

PKH26 labeling of extracellular vesicles: Characterization and cellular internalization of contaminating PKH26 nanoparticles

Dominkuš, P. P., Stenovec, M., Sitar, S., Lasič, E., Zorec, R., Plemenitaš, A., Žagar, E., Kreft, M., & Lenassi, M. (2018) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2018.03.013 · Cited by 358 · see citing works

Bovine milk exosomes contain microRNA and mRNA and are taken up by human macrophages

Izumi, H., Tsuda, M., Sato, Y., Kosaka, N., Ochiya, T., Iwamoto, H., Namba, K., & Takeda, Y. (2015) · Journal of Dairy Science · doi:10.3168/jds.2014-9076 · Cited by 357 · see citing works

Serum Levels of microRNAs in Patients with Heart Failure

Goren, Y., Kushnir, M., Zafrir, B., et al. (2011) · European Journal of Heart Failure · doi:10.1093/eurjhf/hfr155 · Cited by 354 · see citing works

What Are We Looking At? Extracellular Vesicles, Lipoproteins, or Both?

Simonsen, J. B. (2017) · Circulation Research · doi:10.1161/circresaha.117.311767 · Cited by 353 · see citing works

Mast Cell-Dependent B and T Lymphocyte Activation Is Mediated by the Secretion of Immunologically Active Exosomes

Skokos, D., Panse, S. L., Villa, I., Rousselle, J.-C., Péronet, R., David, B., Namane, A., & Mécheri, S. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.2.868 · Cited by 333 · see citing works

Fundamentals of cDNA microarray data analysis

Leung, Y. F., & Cavalieri, D. (2003) · Trends in Genetics · doi:10.1016/j.tig.2003.09.015 · Cited by 333 · see citing works

Recognition and phagocytosis of cells undergoing apoptosis

Savill, J. (1997) · British Medical Bulletin · doi:10.1093/oxfordjournals.bmb.a011626 · Cited by 329 · see citing works

Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

Benedikter, B. J., Bouwman, F. G., Vajen, T., Heinzmann, A. C. A., Grauls, G., Mariman, E. C. M., Wouters, E. F. �., Savelkoul, P. H. M., López‐Iglesias, C., Koenen, R. R., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-15717-7 · Cited by 329 · see citing works

High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets

Kiss, M. M., Ortoleva-Donnelly, L., Beer, N. R., Warner, J. B., Bailey, C. G., Colston, B. W., Rothberg, J. M., Link, D. R., & Leamon, J. H. (2008) · Analytical Chemistry · doi:10.1021/ac801276c · Cited by 327 · see citing works

Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content

Smith, Z. J., Lee, C., Rojalin, T., Carney, R. P., Hazari, S., Knudson, A., Lam, K. S., Saari, H., Lázaro‐Ibáñez, E., Viitala, T., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.28533 · Cited by 322 · see citing works

The Roles of Tumor-Derived Exosomes in Cancer Pathogenesis

Yang, C., & Robbins, P. D. (2011) · Clinical and Developmental Immunology · doi:10.1155/2011/842849 · Cited by 318 · see citing works

A rigorous method to enrich for exosomes from brain tissue

Vella, L. J., Scicluna, B. J., Cheng, L., Bawden, E., Masters, C. L., Ang, C., Williamson, N. A., McLean, C., Barnham, K. J., & Hill, A. F. (2017) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2017.1348885 · Cited by 312 · see citing works

A novel multiplex bead‐based platform highlights the diversity of extracellular vesicles

Koliha, N., Wiencek, Y., Heider, U., Jüngst, C., Kladt, N., Krauthäuser, S., Johnston, I., Bosio, A., Schauss, A. C., & Wild, S. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.29975 · Cited by 307 · see citing works

DNA fragmentation in apoptosis

ZHANG, J. H., & Xu, M. (2000) · Cell Research · doi:10.1038/sj.cr.7290049 · Cited by 301 · see citing works

MiRNA-Sequence Indicates That Mesenchymal Stem Cells and Exosomes Have Similar Mechanism to Enhance Cardiac Repair

Shao, L., Zhang, Y., Lan, B.-B., Wang, J., Zhang, Z., Zhang, L., Xiao, P., Meng, Q., Geng, Y., Yu, X.-Y., et al. (2017) · BioMed Research International · doi:10.1155/2017/4150705 · Cited by 300 · see citing works

High-throughput screening identified selective inhibitors of exosome biogenesis and secretion: A drug repurposing strategy for advanced cancer

Datta, A., Kim, H., McGee, L. E., Johnson, A. E., Talwar, S., Marugán, J., Southall, N. T., Hu, X., Lal, M. A., Mondal, D., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-26411-7 · Cited by 297 · see citing works

Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting

van Balkom, B. W. M., Eisele, A. S., Pegtel, D. M., Bervoets, S., & Verhaar, M. C. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.26760 · Cited by 294 · see citing works

Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales

Ludwig, A., de Miroschedji, K., Doeppner, T. R., Börger, V., Ruesing, J., Rebmann, V., Durst, S., Jansen, S., Bremer, M., Behrmann, E., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1528109 · Cited by 294 · see citing works

Quantitative Sizing of Nano/Microparticles with a Tunable Elastomeric Pore Sensor

Vogel, R., Willmott, G. R., Kozak, D., Roberts, G. S., Anderson, W., Groenewegen, L. G., Glossop, B., Barnett, A., Turner, A., & Trau, M. (2011) · Analytical Chemistry · doi:10.1021/ac200195n · Cited by 287 · see citing works

Exosomes in the Pathology of Neurodegenerative Diseases

Howitt, J. A., & Hill, A. F. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r116.757955 · Cited by 287 · see citing works

Ticket to a bubble ride: Cargo sorting into exosomes and extracellular vesicles

Anand, S., Samuel, M., Kumar, S., & Mathivanan, S. (2019) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2019.02.005 · Cited by 284 · see citing works

Exosomes Isolated From Human Umbilical Cord Mesenchymal Stem Cells Alleviate Neuroinflammation and Reduce Amyloid-Beta Deposition by Modulating Microglial Activation in Alzheimer’s Disease

Ding, M., Shen, Y., Wang, P., Xie, Z., Xu, S., Zhu, Z., Wang, Y., Lyu, Y., Dewei, W., Xu, L., et al. (2018) · Neurochemical Research · doi:10.1007/s11064-018-2641-5 · Cited by 279 · see citing works

Paracrine induction of endothelium by tumor exosomes

Hood, J. L., Pan, H., Lanza, G. M., & Wickline, S. A. (2009) · Laboratory Investigation · doi:10.1038/labinvest.2009.94 · Cited by 273 · see citing works

Emerging Prospects of Exosomes for Cancer Treatment: From Conventional Therapy to Immunotherapy

Nam, G., Choi, Y., Kim, G. B., Kim, S., Kim, S. A., & Kim, I. (2020) · Advanced Materials · doi:10.1002/adma.202002440 · Cited by 266 · see citing works

Extracellular vesicles as a platform for membrane‐associated therapeutic protein delivery

Yang, Y., Hong, Y., Cho, E., Kim, G. B., & Kim, I. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1440131 · Cited by 259 · see citing works

Detecting Exosomes Specifically: A Multiplexed Device Based on Alternating Current Electrohydrodynamic Induced Nanoshearing

Vaidyanathan, R., Naghibosadat, M., Rauf, S., Korbie, D. J., Carrascosa, L. G., Shiddiky, M. J. A., & Trau, M. (2014) · Analytical Chemistry · doi:10.1021/ac502082b · Cited by 258 · see citing works

Mesenchymal stem cell-derived exosomes promote neurogenesis and cognitive function recovery in a mouse model of Alzheimer’s disease

Reza-Zaldivar, E. E., Hernández-Sapiéns, M. A., Gutiérrez-Mercado, Y. K., Sandoval-Ávila, S., Gomez-Pinedo, U., Márquez-Aguirre, A. L., Vázquez-Méndez, E., Padilla-Camberos, E., & Canales-Aguirre, A. A. (2019) · Neural Regeneration Research · doi:10.4103/1673-5374.255978 · Cited by 256 · see citing works

L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma

Norman, M., Ter‐Ovanesyan, D., Trieu, W., Lazarovits, R., Kowal, E. J. K., Lee, J.-H., Chen‐Plotkin, A. S., Regev, A., Church, G. M., & Walt, D. R. (2021) · Nature Methods · doi:10.1038/s41592-021-01174-8 · Cited by 255 · see citing works

Mass-Spectrometry-Based Molecular Characterization of Extracellular Vesicles: Lipidomics and Proteomics

Kreimer, S. D., Belov, A. M., Ghiran, I. C., Murthy, S. K., Frank, D. A., & Ivanov, A. R. (2015) · Journal of Proteome Research · doi:10.1021/pr501279t · Cited by 250 · see citing works

Combining Machine Learning and Nanofluidic Technology To Diagnose Pancreatic Cancer Using Exosomes

Ko, J., Bhagwat, N., Yee, S. S., Ortiz, N., Sahmoud, A., Black, T., Aiello, N. M., McKenzie, L., O’Hara, M., Redlinger, C., et al. (2017) · ACS Nano · doi:10.1021/acsnano.7b05503 · Cited by 249 · see citing works

Immune Cell-Derived Extracellular Vesicles – Functions and Therapeutic Applications

Veerman, R. E., Akpinar, G. G., Eldh, M., & Gabrielsson, S. (2019) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2019.02.003 · Cited by 249 · see citing works

An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker

Goto, T., Fujiya, M., Konishi, H., Sasajima, J., Fujibayashi, S., Hayashi, A., Utsumi, T., Sato, H., Iwama, T., Ijiri, M., et al. (2018) · BMC Cancer · doi:10.1186/s12885-018-4006-5 · Cited by 248 · see citing works

A Comparison of Traditional and Novel Methods for the Separation of Exosomes from Human Samples

Yu, L., Zhu, J., Liu, J., Jiang, F., Ni, W., Qu, L., Ni, R., Lu, C., & Xiao, M. (2018) · BioMed Research International · doi:10.1155/2018/3634563 · Cited by 246 · see citing works

Plasma neuronal exosomes serve as biomarkers of cognitive impairment in HIV infection and Alzheimer’s disease

Pulliam, L., Sun, B., Mustapić, M., Chawla, S., & Kapogiannis, D. (2019) · Journal of NeuroVirology · doi:10.1007/s13365-018-0695-4 · Cited by 246 · see citing works

Extracellular vesicles as circulating cancer biomarkers: opportunities and challenges

Lane, R. E., Korbie, D. J., Hill, M. M. C., & Trau, M. (2018) · Clinical and Translational Medicine · doi:10.1186/s40169-018-0192-7 · Cited by 245 · see citing works

Towards traceable size determination of extracellular vesicles

Varga, Z., Yuana, Y., Grootemaat, A. E., van der Pol, E., Gollwitzer, C., Krumrey, M. K., & Nieuwland, R. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.23298 · Cited by 243 · see citing works

CNS-Derived Blood Exosomes as a Promising Source of Biomarkers: Opportunities and Challenges

Hornung, S., Dutta, S., & Bitan, G. (2020) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2020.00038 · Cited by 239 · see citing works

Biochemical and Biologic Characterization of Exosomes and Microvesicles as Facilitators of HIV-1 Infection in Macrophages

Kadiu, I., Narayanasamy, P., Dash, P. K., Zhang, W., & Gendelman, H. E. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1102244 · Cited by 230 · see citing works

Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy

Moon, P., Lee, J., You, S., Kim, T., Cho, J., Kim, I., Kwon, T., Kim, C., Park, S.-H., Hwang, D., et al. (2011) · PROTEOMICS · doi:10.1002/pmic.201000443 · Cited by 230 · see citing works

Microfluidic approaches for isolation, detection, and characterization of extracellular vesicles: Current status and future directions

Gholizadeh, S., Draz, M. S., Zarghooni, M., Sanati‐Nezhad, A., Ghavami, S., Shafiee, H., & Akbari, M. (2016) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2016.12.062 · Cited by 229 · see citing works

The role of extracellular vesicles in COVID-19 virus infection

Hassanpour, M., Rezaie, J., Nouri, M., & Panahi, Y. (2020) · Infection Genetics and Evolution · doi:10.1016/j.meegid.2020.104422 · Cited by 229 · see citing works

Exosome separation using microfluidic systems: size‐based, immunoaffinity‐based and dynamic methodologies

Yang, F., Liao, X., Tian, Y. Y., & Li, G. (2017) · Biotechnology Journal · doi:10.1002/biot.201600699 · Cited by 226 · see citing works

Characterization of RNA in exosomes secreted by human breast cancer cell lines using next-generation sequencing

Jenjaroenpun, P., Kremenska, Y., Nair, V. M., Kremenskoy, M., Joseph, B., & Kurochkin, I. V. (2013) · PeerJ · doi:10.7717/peerj.201 · Cited by 224 · see citing works

Exosome-Based Cancer Therapy: Implication for Targeting Cancer Stem Cells

Wang, J., Zheng, Y., & Zhao, M. L. (2017) · Frontiers in Pharmacology · doi:10.3389/fphar.2016.00533 · Cited by 224 · see citing works

Methods and Technologies for Exosome Isolation and Characterization

Zhang, M., Jin, K., Gao, L., Zhang, Z., Li, F., Zhou, F., & Zhang, L. (2018) · Small Methods · doi:10.1002/smtd.201800021 · Cited by 223 · see citing works

Tau in neurodegenerative disease

Gao, Y.-L., Wang, N., Sun, F.-R., Cao, X.-P., Zhang, W., & Yu, J.-T. (2018) · Annals of Translational Medicine · doi:10.21037/atm.2018.04.23 · Cited by 222 · see citing works

Characterizing Extracellular Vesicles and Their Diverse RNA Contents

Veziroglu, E. M., & Mias, G. I. (2020) · Frontiers in Genetics · doi:10.3389/fgene.2020.00700 · Cited by 222 · see citing works

Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

Zhang, S., Garcia-D'Angeli, A., Brennan, J. P., & Huo, Q. (2013) · The Analyst · doi:10.1039/c3an01835k · Cited by 219 · see citing works

Exosomes in inflammation and role as biomarkers

Console, L., Scalise, M., & Indiveri, C. (2018) · Clinica Chimica Acta · doi:10.1016/j.cca.2018.11.009 · Cited by 216 · see citing works

Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties

Osteikoetxea, X., Balogh, A., Szabó-Taylor, K., Németh, A., Szabó, T. G., Pálóczi, K., Sódar, B. W., Kittel, Á., György, B., Pállinger, É., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0121184 · Cited by 212 · see citing works

Methods for the physical characterization and quantification of extracellular vesicles in biological samples

Rupert, D. L. M., Claudio, V., Lässer, C., & Bally, M. (2016) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2016.07.028 · Cited by 212 · see citing works

Cardiac myocyte exosomes: stability, HSP60, and proteomics

Malik, Z. A., Kott, K. S., Poe, A. J., Kuo, T., Chen, L., Ferrara, K. W., & Knowlton, A. A. (2013) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00835.2012 · Cited by 209 · see citing works

Lipid Raft Endocytosis and Exosomal Transport Facilitate Extracellular Trafficking of Annexin A2

Valapala, M., & Vishwanatha, J. K. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.271155 · Cited by 209 · see citing works

CNS tau efflux via exosomes is likely increased in Parkinson's disease but not in Alzheimer's disease

Shi, M., Kovac, A., Korff, A., Cook, T. J., Ginghina, C., Bullock, K. M., Yang, L., Stewart, T., Zheng, D., Aro, P., et al. (2016) · Alzheimer's &amp; Dementia · doi:10.1016/j.jalz.2016.04.003 · Cited by 206 · see citing works

Proteomics characterization of exosome cargo

Schey, K. L., Luther, J. M., & Rose, K. L. (2015) · Methods · doi:10.1016/j.ymeth.2015.03.018 · Cited by 201 · see citing works

miR-212 and miR-132 Are Downregulated in Neurally Derived Plasma Exosomes of Alzheimer’s Patients

Cha, D. J., Mengel, D., Mustapić, M., Liu, W., Selkoe, D. J., Kapogiannis, D., Galasko, D. R., Rissman, R. A., Bennett, D. A., & Walsh, D. M. (2019) · Frontiers in Neuroscience · doi:10.3389/fnins.2019.01208 · Cited by 200 · see citing works

Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration

Choi, J. S., Yoon, H. I., Lee, K. S., Choi, Y. C., Yang, S. H., Kim, I.-S., & Cho, Y. W. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.12.018 · Cited by 197 · see citing works

Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes

Li, K., Wong, D., Hong, K. Y., & Raffaı̈, R. L. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-7652-2_7 · Cited by 191 · see citing works

FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for Oleaginous Fungi

Forfang, K., Zimmermann, B., Kósa, G., Köhler, A., & Shapaval, V. (2017) · PLoS ONE · doi:10.1371/journal.pone.0170611 · Cited by 190 · see citing works

Exosomes in atherosclerosis: performers, bystanders, biomarkers, and therapeutic targets

Wang, C., Li, Z., Liu, Y., & Yuan, L. (2021) · Theranostics · doi:10.7150/thno.56035 · Cited by 190 · see citing works

Exosome for mRNA delivery: Strategies and therapeutic applications

Iqbal, Z., Rehman, K., Mahmood, A., Shabbir, M., Liang, Y., Duan, L., & Zeng, H. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02634-x · Cited by 144 · see citing works

Applications of Plant-Derived Extracellular Vesicles in Medicine

Zhu, Y., Zhao, J., Ding, H., Qiu, M., Xue, L., Ge, D., Wen, G., Ren, H., Li, P., & Wang, J. (2024) · MedComm · doi:10.1002/mco2.741 · Cited by 75 · see citing works

Exosomes to exosome-functionalized scaffolds: a novel approach to stimulate bone regeneration

Deng, L., Liu, Y., Wu, Q., Lai, S., Yang, Q., Mu, Y., & Dong, M. (2024) · Stem Cell Research &amp; Therapy · doi:10.1186/s13287-024-04024-4 · Cited by 32 · see citing works

Isolator-based point-of-care manufacturing: a practical solution for GMP-compliant cell and extracellular vesicles therapy production

Chiu, Y.-S., Wang, K.-L., & Wu, K.-L. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1644318 · Cited by 17 · see citing works

Therapeutic Strategies and Enhanced Production of Stem Cell-Derived Exosomes for Tissue Regeneration

Kim, H., Kim, D., Kim, W., Lee, S., Gwon, Y., Park, S., & Kim, J. (2023) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2022.0118 · Cited by 17 · see citing works

Glioblastoma exosomes reprogramming the tumor microenvironment and evading therapeutic challenges

Tatode, A., Premchandani, T., Chaudhary, A. A., Ali, M. A. M., Qutub, M., Taksande, J., Khan, R., Hussain, U. M., & Umekar, M. (2026) · Molecular Biology Reports · doi:10.1007/s11033-026-11526-2 · Cited by 3 · see citing works

The launch of Journal of Extracellular Vesicles (JEV), the official journal of the International Society for Extracellular Vesicles – about microvesicles, exosomes, ectosomes and other extracellular vesicles

Lötvall, J. O., Rajendran, L., Gho, Y. S., Théry, C., Wauben, M. H. M., Raposo, G., Sjöstrand, M., Taylor, D. D., Telemo, E., & Breakefield, X. O. (2012) · Journal of Extracellular Vesicles · doi:10.3402/jev.v1i0.18514 · Cited by 22 · see citing works

Exosomes/EVs: MSC-NTF DERIVED SMALL EXTRACELLULAR VESICLES DISPLAY SUPERIOUR MACROPHAGE IMMUNOMODULATION COMPARED WITH VESICLES DERIVED FROM NAIVE MSC

Dekel, C., Kaspi, H., Kern, R., et al. (2022) · Cytotherapy · doi:10.1016/s1465-3249(22)00264-x

Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics

Yang, D., Zhang, W., Zhang, H., Zhang, F., Chen, L., Ma, L., Larcher, L. M., Chen, S., Liu, N., Zhao, Q., et al. (2020) · Theranostics · doi:10.7150/thno.41580 · Cited by 1,048 · see citing works

Role of Exosomes in Tuberculosis: Looking towards a Future Road Map

Barik, S. K., & Turuk, J. (2023) · Physiology · doi:10.5772/intechopen.111544 · Cited by 1 · see citing works

Unraveling the physiological functions of exosome secretion by tumors

Bobrie, A., & Théry, C. (2013) · OncoImmunology · doi:10.4161/onci.22565 · Cited by 45 · see citing works