Resveratrol suppresses calcium-mediated microglial activation and rescues hippocampal neurons of adult rats following acute bacterial meningitis
Abstract
Acute bacterial meningitis (ABM) is a serious disease with severe neurological sequelae. The intense calcium-mediated microglial activation and subsequently pro-inflammatory cytokine release plays an important role in eliciting ABM-related oxidative damage. Considering resveratrol possesses significant anti-inflammatory and anti-oxidative properties, the present study aims to determine whether resveratrol would exert beneficial effects on hippocampal neurons following ABM. ABM was induced by inoculating Klebsiella pneumoniae into adult rats intraventricularly. The time-of-flight secondary ion mass spectrometry (TOF-SIMS), Griffonia simplicifolia isolectin-B4 (GSA-IB4) and ionized calcium binding adaptor molecule 1 (Iba1) immunohistochemistry, enzyme-linked immunosorbent assay as well as malondialdehyde (MDA) measurement were used to examine the calcium expression, microglial activation, pro-inflammatory cytokine level, and extent of oxidative stress, respectively. In ABM rats, strong calcium signaling associated with enhanced microglial activation was observed in hippocampus. Increased microglial expression was coincided with intense production of pro-inflammatory cytokines and oxidative damage. However, in rats receiving resveratrol after ABM, the calcium intensity, microglial activation, pro-inflammatory cytokine and MDA levels were all significantly decreased. Quantitative data showed that much more hippocampal neurons were survived in resveratrol-treated rats following ABM. As resveratrol successfully rescues hippocampal neurons from ABM by suppressing the calcium-mediated microglial activation, therapeutic use of resveratrol may act as a promising strategy to counteract the ABM-induced neurological damage.
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Bacterial components trigger microglia and phagocytic cells to release pro-inflammatory mediators such as TNF, IL-6 and IL-1B.
“Bacterial components trigger microglia and phagocytic cells to release pro-inflammatory mediators 25 such as TNF, IL-6 and IL-1B.”
From Community-acquired acute bacterial meningitis in adults: a clinical update (2019) · cites this paper as [25]
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- Authors
- Sheu, J.-N., Liao, W.-C., Wu, U.-I., Shyu, L.-Y., Mai, F.-D., Chen, L.-Y., Chen, M.-J., Youn, S.-C., & Chang, H.-M.
- Affiliations
- Chung Shan Medical University Hospital (TW)
- National Taiwan University Hospital (TW)
- Chung Shan Medical University (TW)
- Taipei Medical University (TW)
- Ming Chuan University (TW)
- Corresponding author
- Chang, Hung-Ming
- Venue
- Comparative Immunology Microbiology and Infectious Diseases
- Publisher
- Elsevier BV
- Published
- 2013-03
- Volume
- 36
- Issue
- 2
- Pages
- 137-148
- ISSN
- 0147-9571 (ISSN-L)
- OpenAlex
- W2087873578
- Cited by
- 20
- References
- 52
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 2 · 2018: 3 · 2016: 4 · 2015: 4
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Comparative Immunology Microbiology and Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Keywords
- resveratrol, acute bacterial meningitis, microglial activation, hippocampal neurons, calcium signaling, oxidative stress, inflammatory cytokines, Klebsiella pneumoniae, neuroprotection, neuroinflammation
- MeSH terms
- Resveratrol, Animals, Calcium, Cerebrospinal Fluid, Hippocampus, Male, Neurons, Stilbenes, Cytokines, Meningitis, Bacterial, Microglia, Neuroprotective Agents, Inflammation Mediators, Rats
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