Cited by 20Neuroinflammation and Neurodegeneration MechanismsBacterial Infections and VaccinesSirtuins and Resveratrol in Medicine

Resveratrol suppresses calcium-mediated microglial activation and rescues hippocampal neurons of adult rats following acute bacterial meningitis

Sheu, Ji-Nan, Liao, Wen-Chieh, Wu, Un-In, Shyu, Ling-Yuh, Mai, Fu-Der, Chen, Li-You, Chen, Mei-Jung, Youn, Su-Chung +1 more·2013·Comparative Immunology Microbiology and Infectious Diseases

Abstract

Acute bacterial meningitis (ABM) is a serious disease with severe neurological sequelae. The intense calcium-mediated microglial activation and subsequently pro-inflammatory cytokine release plays an important role in eliciting ABM-related oxidative damage. Considering resveratrol possesses significant anti-inflammatory and anti-oxidative properties, the present study aims to determine whether resveratrol would exert beneficial effects on hippocampal neurons following ABM. ABM was induced by inoculating Klebsiella pneumoniae into adult rats intraventricularly. The time-of-flight secondary ion mass spectrometry (TOF-SIMS), Griffonia simplicifolia isolectin-B4 (GSA-IB4) and ionized calcium binding adaptor molecule 1 (Iba1) immunohistochemistry, enzyme-linked immunosorbent assay as well as malondialdehyde (MDA) measurement were used to examine the calcium expression, microglial activation, pro-inflammatory cytokine level, and extent of oxidative stress, respectively. In ABM rats, strong calcium signaling associated with enhanced microglial activation was observed in hippocampus. Increased microglial expression was coincided with intense production of pro-inflammatory cytokines and oxidative damage. However, in rats receiving resveratrol after ABM, the calcium intensity, microglial activation, pro-inflammatory cytokine and MDA levels were all significantly decreased. Quantitative data showed that much more hippocampal neurons were survived in resveratrol-treated rats following ABM. As resveratrol successfully rescues hippocampal neurons from ABM by suppressing the calcium-mediated microglial activation, therapeutic use of resveratrol may act as a promising strategy to counteract the ABM-induced neurological damage.

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Crossref · 10.1016/j.cimid.2012.11.002

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  • Bacterial components trigger microglia and phagocytic cells to release pro-inflammatory mediators such as TNF, IL-6 and IL-1B.

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Authors
Sheu, J.-N., Liao, W.-C., Wu, U.-I., Shyu, L.-Y., Mai, F.-D., Chen, L.-Y., Chen, M.-J., Youn, S.-C., & Chang, H.-M.
Affiliations
  • Chung Shan Medical University Hospital (TW)
  • National Taiwan University Hospital (TW)
  • Chung Shan Medical University (TW)
  • Taipei Medical University (TW)
  • Ming Chuan University (TW)
Corresponding author
Chang, Hung-Ming
Venue
Comparative Immunology Microbiology and Infectious Diseases
Publisher
Elsevier BV
Published
2013-03
Volume
36
Issue
2
Pages
137-148
ISSN
0147-9571 (ISSN-L)
OpenAlex
W2087873578
Cited by
20
References
52
Citations by year
2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 2 · 2018: 3 · 2016: 4 · 2015: 4
FWCI
0.64
Type
journal article
Language
en
Open access
closed
Locations
  • Comparative Immunology Microbiology and Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
Keywords
resveratrol, acute bacterial meningitis, microglial activation, hippocampal neurons, calcium signaling, oxidative stress, inflammatory cytokines, Klebsiella pneumoniae, neuroprotection, neuroinflammation
MeSH terms
Resveratrol, Animals, Calcium, Cerebrospinal Fluid, Hippocampus, Male, Neurons, Stilbenes, Cytokines, Meningitis, Bacterial, Microglia, Neuroprotective Agents, Inflammation Mediators, Rats

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